IJOHNS  Vol.2 No.4 , July 2013
The Expression of IL-27, Th17 Cells and Treg Cells in Peripheral Blood of Patients with Allergic Rhinitis
Objective: To explore the expression of IL-27, Th17 cells and CD4+CD25+ regulatory T cells (Treg) as well as its associated cytokines in peripheral blood of patients with allergic rhinitis (AR). Method: From March 2012 to May, the peripheral blood of 24 cases of AR patients (AR group) and 16 cases of healthy volunteers (control group) was collected, and the percentage of Th17 cells and Treg cells in the peripheral blood was detected by flow cytometry (FCM); the levels of IL-27, IL-17 and IL-10 in serum was detected by ELISA. Result: The percentage of Th17 cells in AR group and the control group was 1.76% ± 0.60% and 0.59% ± 0.17%, respectively. It was higher in AR group than in control group, and the difference between two groups was statistically significant (P < 0.01); Treg cell percentage in AR group and control group was 1.65% ± 0.79% and 5.03% ± 1.92%, respectively. AR group was significantly lower than the control group, and the difference between two groups was statistically significant (P < 0.01). Serum IL-17 expression level (668.68 ± 62.59) pg/ml in AR group was higher than that of the control group (587.30 ± 28.00) pg/ml, and the difference was statistically significant (P < 0.01); the levels of IL-27 in AR group and the control group were (23.15 ± 10.12) pg/ml and (52.97 ± 10.08) pg/ml, and the difference was statistically significant (P < 0.01); IL-10 expression level (14.29 ± 6.16) pg/ml in AR group serum was lower than that in the control group (31.32 ± 21.20) pg/ml, and the difference between two groups was statistically significant (P < 0.01). In the peripheral blood of AR patients, there was a negative correlation between Th17 cell percentage and Treg cell percentage, IL-10 (r = -0.794, -0.483, P < 0.01), and a negative correlation between IL-27 and Th17 cell percentage, IL-17 (r was -0.758 and -0.519 respectively, P < 0.01). IL-27 was positively correlated with Treg cell percentage and IL-10 (r = 0.722, 0.646, P < 0.01), and percentage of Treg cells and IL-10 was positively correlated (r = 0.622, P < 0.01). There was no correlation between IL-17 and Th17 cell percentage, Treg cell percentage, IL-10 (r = 0.225, -0.183, -0.176, P > 0.05). Conclusion: In the peripheral blood of AR patients there was a reduction of IL-27 level and imbalance of Th17/Treg cell function. IL-27 on Th17/Treg cells adjustment may play an important role on the pathogenesis of the AR.

Cite this paper
X. Huang, P. Li, Q. Yang, Y. Chen and G. Zhang, "The Expression of IL-27, Th17 Cells and Treg Cells in Peripheral Blood of Patients with Allergic Rhinitis," International Journal of Otolaryngology and Head & Neck Surgery, Vol. 2 No. 4, 2013, pp. 138-142. doi: 10.4236/ijohns.2013.24030.
[1]   J. Bousquet, N. Khaltaev, A. A. Cruz, et al., “Allergic Rhinitis and Its Impact on Asthma (ARIA) 2008 Update (in Collaboration with the World Health Organization, GA2LEN and AllerGen),” Allergy, Vol. 63, Suppl. 86, 2008, pp. 8-160. doi:10.1111/j.1398-9995.2007.01620.x

[2]   H. Yoshida, M. Nakaya and Y. Miyazaki, “Interleukin 27: A Double-Edged Sword for Offense and Defense,” Journal of Leukocyte Biology, Vol. 86, No. 6, 2009, pp. 1295-1303. doi:10.1189/jlb.0609445

[3]   L. E. Harrington, R. D. Hatton, P. R. Mangan, et al., “Interleukin 17-Producing CD4+ Effector T Cells Develop via a Lineage Distinct from the T Helper Type 1 and 2 Lineages,” Nature Immunology, Vol. 6, 2005, pp. 1123-1132. doi:10.1038/ni1254

[4]   G. Ciprandi, M. De Amici, G. Murdaca, et al., “Serum Interleukin-17 Levels Are Related to Clinical Severity in Allergic Rhinitis,” Allergy, Vol. 64, No. 9, 2009, pp. 1375-1378. doi:10.1111/j.1398-9995.2009.02010.x

[5]   C. Ozdemir, M. Akdis and C. A. Akdis, “T Regulatory Cells and Their Counterparts: Masters of Immune Regulation,” Clinical & Experimental Allergy, Vol. 39, No. 5, 2009, pp. 626-639. doi:10.1111/j.1365-2222.2009.03242.x

[6]   S. Pflanz, L. Hibbert, J. Mattson, et al., “WSX-1 and Glycoprotein 130 Constitute a Signal-Transducing Receptor for IL-27,” Journal of Immunology, Vol. 172, No. 4, 2004, pp. 2225-2231.

[7]   A. E. Troy, C. Zaph, Y. Du, et al., “IL-27 Regulates Homeostasis of the Intestinal CD4+ Effector T Cell Pool and Limits Intestinal Inflammation in a Murine Model of Colitis,” Journal of Immunology, Vol. 183, No. 3, 2009, pp. 2037-2044. doi:10.4049/jimmunol.0802918

[8]   D. C. Fitzgerald and A. Rostami, “Therapeutic Potential of IL-27 in Multiple Sclerosis?” Expert Opinion on Biological Therapy, Vol. 9, No. 2, 2009, pp. 149-160. doi:10.1517/14712590802646936

[9]   E. Dokmeci, L. Xu, E. Robinson, et al., “EBI3 Deficiency Leads to Diminished T Helper Type 1 and Increased T Helper Type 2 Mediated Airway Inflammation,” Immunology, Vol. 132, No. 4, 2011, pp. 559-566. doi:10.1111/j.1365-2567.2010.03401.x

[10]   H. Fujita, A. Teng, R. Nozawa, et al., “Production of Both IL-27 and IFN-Gamma after the Treatment with a Ligand for Invariant NK T Cells Is Responsible for the Suppression of Th2 Response and Allergic Inflammation in Amouse Experimental Asthma Model,” Journal of Immunology, Vol. 183, No. 1, 2009, pp. 254-260. doi:10.4049/jimmunol.0800520

[11]   S. Romagnani, “Regulation of the T Cell Response,” Clinical & Experimental Allergy, Vol. 36, No. 11, 2006, pp. 1357-1366. doi:10.1111/j.1365-2222.2006.02606.x

[12]   P. W. Hellings, A. Kasran, Z. Liu, et al., “Interleukin-17 Orchestrates the Granulocyte Influx into Airways after Allergen Inhalation in a Mouse Model of Allergic Asthma,” American Journal of Respiratory Cell and Molecular Biology, Vol. 28, No. 1, 2003, pp. 42-50.

[13]   S. Sergejeva, S. Ivanov, J. Lötvall and A. Lindén, “Interleukin-17 as a Recruitment and Survival Factor for AIR-WAY Macrophages in Allergic Airway Inflammation,” American Journal of Respiratory Cell and Molecular Biology, Vol. 33, No. 3, 2005, pp. 248-253.

[14]   C. M. Hawrylowicz and A. O’Garra, “Potential Role of Interleukin-10-Secreting Regulatory T Cells in Allergy and Asthma,” Nature Reviews Immunology, Vol. 5, No. 4, 2005, pp. 271-283. doi:10.1038/nri1589

[15]   G. Ciprandi, G. Filaci, F. Battaglia, et al., “Peripheral Th17 Cells in Allergic Rhinitis: New Evidence,” International Immunopharmacology, Vol. 10, No. 2, 2010, pp. 226-229. doi:10.1016/j.intimp.2009.11.004

[16]   G. Xu, Z. Mou, H. Jiang, et al., “A Possible Role of CD4+CD25+ T Cells as Well as Transcription Factor Foxp3 in the Dysregulation of Allergic Rhinitis,” Laryngoscope, Vol. 117, No. 5, 2007, pp. 876-880. doi:10.1097/MLG.0b013e318033f99a

[17]   M. Batten, J. Li, S. Yi, et al., “Interleukin 27 Limits Autoim-Mune Encephalomyelitis by Suppressing the Development of Interleukin 17-Producing T Cells,” Nature Immunology, Vol. 7, No. 9, 2006, pp. 929-936. doi:10.1038/ni1375

[18]   A. Awasthi, Y. Carrier, J. P. Peron, et al., “A Dominant Fonction for Interleukin 27 in Generating Interleukin 10-Producing Anti-Inflammatory T Cells,” Nature Immunology, Vol. 8, No. 12, 2007, pp. 1380-1389. doi:10.1038/ni1541

[19]   A. V. Villarino, J. Larkin III, C. J. Saris, et al., “Positive and Negative Regulation of the IL-27 Receptor during Lymphoid Cell Activation,” Journal of Immunology, Vol. 174, No. 12, 2005, pp. 7684-7691.

[20]   R. X. Wang, C. R. Yu, R. M. Mahdi, et al., “Novel IL27p28/IL12p40 Cytokine Suppressed Experimental Autoimmune Uveitis by Inhibiting Autoreactive Th1/Th17 Cells and Promoting Expansion of Regulatory T Cells,” Journal of Biological Chemistry, Vol. 287, No. 43, 2012, pp. 36012-36021. doi:10.1074/jbc.M112.390625