A testing method for N-nitrosamines and N-nitrosatable substances in rubber teats and soothers was modified. N-Nitrosamines are generally analyzed using either a nitrogen chemiluminescence detector (NCD) or a thermal energy analyzer (TEA). However, because few testing laboratories are equipped with these devices, it is difficult to conduct these tests. Therefore, an analysis method for N-nitrosamines using the more widespread gas chromatography-mass spectrometry (GC-MS) method was improved. In addition, EN 12868 was used to prepare the test solutions because of its worldwide use and compliance with EU regulations. Using GC-MS, EN 12868 method targeting ten kinds of N-nitrosamines was modified. The determination limits of the method were 1.0 -1.5 μg/kg for N-nitrosamines and 4 -6 μg/kg for N-nitrosatable substances. Quantification was possible at 1/5 or less and 1/15 or less, respectively, of the regulation values listed in EU Directive 93/11/EEC. In terms of application, there were no problems with the selectivity of the detector. The recoveries were 58% -109% for N-nitrosamines and 59% -102% for N-nitrosatable substances. Screening and verification were possible by measuring the amount of secondary amines in the boiled solution and migration solution.
 B. L. Pool, et al., “Detection of Mutations in Bacteria and of DNA Damage and Amplified DNA Sequences in Mammalian Cells as a Systematic Test Strategy for Elucidating Biological Activities of Chemical Carcinogens,” Food Chemical Toxicology, Vol. 24, No. 6-7, 1986, pp. 685-691. doi:10.1016/0278-6915(86)90158-4
 B. G. Boyes, et al., “Evaluation of Genotoxicity of N-Nitrosodibenzylamine in Chinese Hamster V79 Cells and Salmonella,” Mutation Research, Vol. 241, No. 4, 1990, pp. 379-385. doi:10.1016/0165-1218(90)90068-D
 J. Jiao, et al., “Analysis of Tissue-Specific lacZ Mutations Induced by N-Nitrosodibenzylamine in Transgenic Mice,” Carcinogenesis, Vol. 18, No. 11, 1997, pp. 2239- 2245. doi:10.1093/carcin/18.11.2239
 The Commission of the European Communities, “Commission Directive 93/11/EEC Concerning the Release of the N-Nitrosamines and N-Nitrosatable Substances from Elastomer or Rubber Teats and Soothers,” 1993.
 European Committee for Standardization, “EN 12868 Child Use and Care Articles-Methods for Determining the Release of N-Nitrosamines and N-Nitrosatable Substances from Elastomer or Rubber Teats and Soothers,” 1999.
 M. Takeuchi, et al., “Determination of Volatile N-Nitrosamines in Rubber Nipples by Gas Chromatography Using Thermal Energy Analyzer,” Analytical Sciences, Vol. 2, No. 6, 1986, pp. 577-580. doi:10.2116/analsci.2.577
 N. P. Sen, et al., “Improved Method for Determination of Volatile Nitrosamines in Baby Bottle Rubber Nipples and Pacifiers,” Journal—Association of Official Analytical Chemists, Vol. 70, No. 3, 1987, pp. 434-438.
 K. Mizuishi, et al., “Migration of Secondary Amines from Rubber Nipples and Pacifiers, and Formation of Nitrosamines,” Annual Report of Tokyo Metropolitan Institute of Public Health, Vol. 35, 1984, pp. 127-132.
 K. Mizuishi, et al., “Hygienic Chemical Survey on Rubber Nursing Products Migrating and Material Test for Nipples and Pacifiers,” Annual Report of Tokyo Metropolitan Institute of Public Health, Vol. 37, 1986, pp. 145- 148.
 K. Mizuishi, et al., “Hygienic Chemical Survey on Rubber Products Content of N-Nitrosamines in Nipples and Pacifiers,” Annual Report of Tokyo Metropolitan Institute of Public Health, Vol. 39, 1988, pp. 97-100.
 T. Tatsuno, et al., “Hygienic Studies on Rubber Nipples (I). Secondary Amines in Commercial Rubber Nipples and Formation of Nitrosamines,” Bulletin of National Institute of Hygienic Sciences, Vol. 100, 1982, pp. 62-71.
 K. Bouma, et al., “Migration of N-Nitrosamines, N-Nitrosatable Substances and 2-Mercaptobenzthiazol from Baby Bottle Teats and Soothers: A Dutch Retail Survey,” Food Additives and Contaminants, Vol. 20, No. 9, 2003, pp. 853-858. doi:10.1080/0265203031000156105
 B. J. Sanchez, et al., “Comparison of the Sensitivities of Seven N-Nitrosamines in Prescreened Waters Using an Automated Preconcentration System and Gas Chromatography with Different Detectors,” Journal of Chromatography A, Vol. 1154, No. 1-2, 2007, pp. 66-73.
 J. H. Sung, et al., “Liquid Chromatography-Tandem Mass Spectrometry Determination of N-Nitrosamines Released from Rubber or Elastomer Teats and Soothers,” Food Additives and Contaminants, Vol. 27, No. 12, 2010, pp. 1745-1754. doi:10.1080/19440049.2010.508184
 D. Feng, et al., “Evaluation of Simulant Migration of Volatile Nitrosamines from Latex Gloves and Balloons by HS-SPME-GC-MS,” Journal of Chromatographic Science, Vol. 50, No. 8, 2012, pp. 733-738. doi:10.1093/chromsci/bms057