Possible early diagnostic application of optical methods (dielectrophoresis, spectral and imaging ellipsometry, Fourier-transform infrared spectroscopy, Raman spectroscopy) in studies of red blood cells and serum of patients with diffuse liver disease with varying degrees of fibrosis has been evaluated. Application of combined optical methods was confirmed to significantly improve the performance of sensitivity, specificity, and accuracy index as well as to achieve the reliable results in diagnosis of both severe fibrosis and slight ulterior liver fibrosis. Identified diagnostic potential of optical methods can be effectively utilized in noninvasive screening evaluation of stages of diffuse liver disease of various geneses.
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M. Kruchinina, M. Voevoda, S. Peltek, S. Kurilovich, A. Gromov, V. Kruchinin, S. Rykhlitsky, V. Volodin and V. Generalov, "Application of Optical Methods in Blood Studies upon Evaluation of Severity Rate of Diffuse Liver Pathology," Journal of Analytical Sciences, Methods and Instrumentation, Vol. 3 No. 2, 2013, pp. 115-123. doi: 10.4236/jasmi.2013.32014.
 V. T. Ivashkin and T. L. Lapina, “Gastroenterologiya. Natsionalnoyerukovodstvo (Gastroenterology. National Guide),” GEOTAR-Media, Moscow, 2008.
 S. D. Podymova, “Liver Diseases,” Meditsina, Moscow, 1993.
 M. I. Voevoda, S. E. Peltek, M. V. Kruchinina, S. A. Kurilovich, V. N. Kruchinin, K. P. Mogilnikov and S. V. Rykhlitskii, “Studying Thin Films Obtained through Centrifugation of the Human Blood Serum by Methods of Spectral Ellipsometry and Infrared Spectroscopy,” Optoelectronics, Instrumentation and Data Processing, Vol. 46, No. 4, 2010, pp. 382-393.
 M. V. Kruchinina, S. A. Kurilovich, M. V. Parulikova, A. A. Gromov, T. S. Bakirov, V. M. Generalov and A. V. Pak, “Viscoelastic and Electrical Properties of Red Blood Cells in Patients with Various Degrees of Liver Fibrosis,” Vestnik NGU, Vol. 4, 2005, pp. 43-52.
 V. M. Generalov, M. V. Kruchinina, A. G. Durymanov, A. A. Medvedev, A. S. Safatov, A. N. Sergeev, G. A. Buryk, S. A. Kurilovich and A. A. Gromov, “Dielectrophoresis in Diagnosis of Infectious and Noninfectious Diseases,” ZERIS, Novosibirsk, 2011.
 Y. Dimitriev, Y. Ivanova and R. Iordanova, “History of Sol-Gel Science and Technology (Review),” Journal of the University of Chemical technology and Metallurgy, Vol. 43, No. 2, 2008, pp. 181-192.
 K. Vorotilov, V. Petrovsky and V. Vasiljev, “Spin Coating Process of Sol-Gel Silicate Films Deposition: Affect of Spin Speed and Processing Temperature,” Journal of Sol-Gel Science and Technology, Vol. 5, No. 3, 1995, pp. 173-183. doi:10.1007/BF00487014
 S. V. Ryhlitsky, E. V. Spesivtsev, V. A. Shvets and V. Yu. Prokopiev, “Spectral Ellipsometric Complex Ellipse, 1771,” Pribory I Tekhnika Eksperimenta, Vol. 2, 2007, pp. 160-161.
 G. I. Kozinets and V. A. Makarov, “Issledovaniyesistemykrovi v Klinicheskoypraktike (Studies of Blood System in Clinical Practice),” Triada-X, Moscow, 1997.
 R. Manoharan, Y. Wang and M. S. Feld, “Histochemical Analysis of Biological Tissues Using Raman Spectroscopy,” Spectrochimica Acta Part A, Vol. 52, No. 2, 1996, pp. 215-249. doi:10.1016/0584-8539(95)01573-6
 T. C. B. Schut, G. J. Puppels, Y. M. Kraan, J. Greve, L. van der Maas and C. G. Figdor, “Intracellular Carotenoid Levels Measured by Raman Microspectroscopy: Comparison of Lymphocytes from Lung Cancer Patients and Healthy Individuals,” International Journal of Cancer, Predictive Oncology, Vol. 74, No. 1, 1997, pp. 20-25.
 E. A. Chernitsky and A. V. Vorobey, “Structure and Function of Erythrocyte Membranes,” Nauka I Takhnika, Minsk, 1981.
 M. V. Kruchinina, “Structural and Functional Properties of Red Blood Cells in Somatic Pathology of Alcoholic Genesis,” Avtoref. dis. kand. Med. Nauk, Novosibirsk, 1994.
 V. V. Novitsky, N. V. Ryazantseva and E. A. Stepovaya, “Physiology and Pathophysiology of the Erythrocyte,” Izdatelstvo TGU, Tomsk, 2004.
 T. J. Leonova, “On the Issue of Erythrocytic Mechanism of Control of Cholesteremia in Cases of Experimental Hypercholesteremia and Ischemic Heart Disease,” Avtoref.diss.kand. Med. Nauk, Novosibirsk, 1982.
 F. J. Schiffman, “Pathophysiology of Blood,” Edition Group “BINOM”, Moscow and “Nevsky Dialogue”, Saint-Petersburg, 2000.
 L. Strayer, “Biochemistry,” Mir, Moscow, 1984.
 A. I. Miroshnikov, V. M. Fomchenkov and A. Yu. Ivanov, “Electrophysical Analysis and Separation of Cells,” Nauka, Moscow, 1986.
 A. L. Chizhevsky, “Biophysical Mechanisms of Erythrocyte Sedimentation Rate,” Nauka, Novosibirsk, 1980.
 V. A. Levtov, R. S. A. Egirer and N. H. Shadrina, “Blood Rheology,” Meditsina, Moscow, 1982.
 T. Grinhald, “Evidence-Based Medicine,” GEO-TARG- Media, Moscow, 2004.