OJVM  Vol.3 No.2 , June 2013
Some Characters of Cl. perfringens Isolated from Fresh and Marketed Processed Meat
Abstract: This study was carried out a fresh meat immediately after slaughter and a marketed processed cattlemeat (sausage and minced meat). A total of 530 meat samples were examined for the presence of Cl. perfringens, 423 were from fresh meat obtained immediately after slaughtering (108 cattle meat, 101 sheep meat, 100 camel meat and 114 buffaloe meat) and 107 processed meat (57 from sausage and 50 from minced meat). Cl. perfringens was isolated from 204 (48.2%) of fresh meat samples, 61 (56.5%) from cattle, 53 (52.5%) from sheep meat, 45 (45%) from camel meat and 45 (39.5%) from buffaloe meat. The isolation rate of Cl. perfringens was higher in processed meat, it was isolated from 68 (63.6%) of which 45 (78.9%) from sausage and 23 (46%) in minced meat. The processed meat was found to harbour higher viable count ranging between 4 × 102 - 7 × 106 Cl. perfringens cells/gm meat than that Fresh meat in which the number ranged from 102:5 × 106 cells/gm meat. Typing of isolated strains revealed that the majority of it was of Cl. perfringens type A, 2 of type B, 3 of type C and one type D. Sixty strains of Cl. perfringens type A were randomized and tested for heat resistance at 100℃ and the results were recorded. Production of enterotoxin by 10 strains of Cl. perfringens was performed by ligated ileal loop test in rabbits. It was done by injection of whole culture in skimmed milk, cell extracts and concentrated culture filtrates of the organism in the ileal ligated loop of rabbits and the results were recorded.
Cite this paper: K. Alkheraije, "Some Characters of Cl. perfringens Isolated from Fresh and Marketed Processed Meat," Open Journal of Veterinary Medicine, Vol. 3 No. 2, 2013, pp. 187-191. doi: 10.4236/ojvm.2013.32029.

[1]   J. G. Songer, “Clostridial Enteric Diseases of Domestic Animals,” Clinical Microbiology Reviews, Vol. 9, No. 2, 1996, pp. 216-234.

[2]   B. A. McClane, “Clostridial Enterotoxin,” In: P. Durre, Ed., Handbook on Clostridia, CRC Press, Boca Raton, 2005, pp. 385-406. doi:10.1201/9780203489819.ch18

[3]   M. M. Effat, Y. A. Abdallah, M. F. Soheir and R. M. M. Rady, “Characterization of Cl. perfringens Field Isolates, Implicated, in Necrotic Enteritis Outbreaks on Private Broiler Farms in Cairo, by Multiplex PCR,” African Journal of Microbiology, 2007, pp. 29-32.

[4]   L. D. S. Smith, “The Pathogenic Anaerobic Bacteria,” 2nd Edition, Charles C. Thomas Publisher, 1975.

[5]   J. I. Rood, “Virulence Genes of Cl. perfringens,” Annual Reviews, Vol. 52, 1998, pp. 333-360. doi:10.1146/annurev.micro.52.1.333

[6]   P. Udompijitkul, D. Paredes-Sabja and M. R. Sarker, “Inhibitory Effects of Nisin against Cl. perfringens Food Poisoning and Non-Food-Borne Isolates,” Journal of Food Science, Vol. 1, 2012, pp. 51-56.

[7]   A. T. Willis and G. Hobbs, “Some New Media for the Isolation and Identification of Clostridia,” The Journal of Pathology and Bacteriology, Vol. 77, No. 2, 1959, pp. 511-521. doi:10.1002/path.1700770223

[8]   M. Sterne and I. Batty, “Pathogenic Clostridia,” 1st Edition, Butterworth, London, Boston, 1975.

[9]   E. Y. M. EL-Naenaeey, “Occurrence of Enterotoxin Strains of Cl. perfringens Type A,” Ph.D. Dissertation (Microbiology), Faculty of Veterinary Medicine, Zagazig University, Sharkeya, 1989.

[10]   C. L. Duncan and D. H. Strong, “Improved Medium for Sporulation of Cl. perfringens,” Journal of Applied Microbiology, Vol. 16, No. 1, 1968, pp. 82-89.

[11]   K. H. Harry, R. Zhou, L. Kross and S. B. Melville, “Sporulation and Enterotoxin (CPE) Synthesis Are Controlled by the Sporulation-Specific Sigma Factor SigE and SigK in Cl. perfringens,” Bacteriology, Vol. 191, No. 8, 2009, pp. 2728-2742. doi:10.1128/JB.01839-08

[12]   A. Z. Hussein, “The Physical Conditions of Cattles before Slaughtering and Its Relationship to Probable Isolation of Cl. perfringens from the Carcasses,” KandedatnayK, Varonish Agriculture Institute, USSR, 1977.

[13]   C. L. Duncan, H. Sugiyama and D. H. Strong, “Rabbitileal Loop Response to Strains of Cl. perfringens,” Journal of Bacteriology, Vol. 95, No. 5, 1968, pp. 1560-1566.

[14]   C. L. Duncan and D. H. Strong, “Experimental Production of Diarrhea in Rabbits with Cl. perfringens,” Canadian Journal of Microbiology, Vol. 15, No. 7, 1969, pp. 765-770. doi:10.1139/m69-134

[15]   C. L. Duncan and D. H. Strong, “Ileal Loop Fluid Accumulation and Production of Diarrhea in Rabbits by Cell-Free Products of Cl. perfringens,” Journal of Bacteriology, Vol. 100, No. 1, 1969, pp. 86-94.

[16]   C. L. Duncan, D. H. Strong and M. Sebald, “Sporulation and Enterotoxin Production by Mutants of Cl. perfringens,” Journal of Bacteriology, Vol. 110, No. 1, 1972, pp. 378-391.

[17]   G. W. Sendercor, “Statistical Methods,” 5th Edition, Iowa University, Iowa, 1961.

[18]   J. L. Smart, T. A. Roberts, M. F. Stringer and N. Shah, “The Incidence and Serotypes of Cl. perfringens on Beef, Pork and Lamb Carcasses,” Journal of Applied Microbiology, Vol. 46, No. 2, 1979, pp. 377-383. doi:10.1111/j.1365-2672.1979.tb00834.x

[19]   M. T. Shouman, M. M. EL. Bardisy and A. Z. Hussein, “Enumeration and Incidence of Lecithinase Positive Cl. perfringens in Local Fresh and Imported Frozen Meat,” The Journal of the Egyptian Medical Association, Vol. 45, No. 1, 1985, pp. 217-224.

[20]   J. F. Foster, J. L. Flower and W. C. Ladiges, “Bacteriological Survey of Raw Gound Beef,” Journal of Food Protection, Vol. 40, No. 11, 1977, pp. 790-794.

[21]   H. Youssef, “Incidence of Cl. perfringens in Meat Products in Assiut City,” Assuit Veterinary Medical Journal, Vol. 12, No. 23, 1984, pp. 145-147.

[22]   D. H. Strong, J. C. Canada and B. B. Griffiths, “Incidence of Cl. perfringens in American Foods,” Journal of Applied Microbiology, Vol. 11, 1963, pp. 42-44.

[23]   A. Z. Hussein and I. Farrag, “Incidence of Cl. perfringens in Beef,” Personal Communication, 1980.

[24]   M. M. M. EL-Bardisy, “Bacteriological Studies on Cl. perfringens in Meat,” M. V. Sc. Thesis (Microbiology), Faculty of Veterinary Medicine, Cairo University, Cairo, 1984.

[25]   S. M. Harmon, D. A. Kautter and J. T. Peeler, “Improved Medium for Enumeration of Cl. perfringens,” Journal of Applied Microbiology, Vol. 22, No. 4, 1971, pp. 688-692.

[26]   A. Deguchi, K. Miyamoto, T. Kuwahara, Y. Miki, I. Kaneko, J. Li, B, A. McClane and S. Akimoto, “Genetic Characterization of Type A Enterotoxigenic Cl. perfringens Strains,” Plos One, Vol. 4, No. 5, 2009, p. 5598. doi:10.1371/journal.pone.0005598

[27]   H. S. Yoo, S. U. Lee, K. Y. Park and Y. H. Park, “Molecular Typing and Epidemiological Survey of Prevalence of Cl. perfringens Type by Multiplex PCR,” Journal of Clinical Microbiology, Vol. 35, No. 1, 1997, pp. 228-232.

[28]   E. A. Wijewanta, “Isolation of Heat-Resistant Cl. perfringens from Healthy Cattle,” Cornell Veterinarian, Vol. 62, No. 1, 1972, pp. 26-31.

[29]   A. H. W. Hauschild, “Clostridium perfringens Enterotoxin,” Journal of Milk and Food Technology, Vol. 34, No. 12, 1971, pp. 596-599.

[30]   R. Skjelkvale, M. F. Stringer and J. L. Smart, “Enterotoxin Production by Lecithinase-Positive and Lecithinase-Negative Cl. perfringens Isolated from Food Poisoning Outbreaks and Other Source,” Journal of Applied Microbiology, Vol. 47, No. 2, 1979, pp. 329-339. doi:10.1111/j.1365-2672.1979.tb01763.x

[31]   L. Niilo, “Enterotoxigenic Cl. perfringens Type A Isolated from Intestinal Contents of Cattle, Sheep and Chickens,” Canadian Journal of Comparative Medicine and Veterinary Science, Vol. 42, 1978, pp. 357-363.