JASMI  Vol.3 No.2 , June 2013
Assessment of Commercial Specific IgE Assay for Detection of Allergens in Allergic Patients
Abstract: Allergy is a serious health problem throughout the world, affecting people of all ages. Allergic diseases such as asthma, rhinitis and atopic dermatitis are becoming an epidemic in our country, the cost of investigating these diseases is increasing and becoming expensively high. There are many ways to explore allergenic antibodies to assess the presence the amount of specific IgE as: Skin test (Prick), Specific IgE (ELISA), RAST Sp.IgE and Elimination Challenge method. Skin test produces pain, local and anaphylactic reaction and patient discomfort, other procedure with highly cost to patient. So, we have built up an evidence to use it in our allergy and immunology clinic has suitable cost for all patients based about for the other tests but it was very simple, accurate, cheap and does not produce any problems to patients. Our test depends on ELISA technique, it measures the quantitative amount of the following different allergens: As regard food allergens: Milk, Eggs, Banana, Maize, Fish, Chocolate, Wheat, Nuts, Strawberry, Shrimps, Spices, with drug as Aspirin. As regard inhalants as: House dust, Mite, Mixed Pollens, Mixed Moulds, Hay dust, Wool, Latex and Cat Hair. 150 allergic patient results of our test compared with specific IgE national kits (ELISA), Sp.IgE (RAST), Skin test and elimination challenge test. The statistical evaluation results as regard senistivity respectively: 88.9%, 89.6%, 91.2%, 71.4%, 93.1%. As regard specificity the results: 93.1%, 94.7%, 95.3%, 65.5%, 91.6%, the latter results showed that our test was in line with all tests. Our test was probably in food, drug and inhalant allergens, it can be also noted that the cheapest and most commercial technique was used, so as to be available immediately in standard laboratory and its reagents, plate and other requirements were prepared locally in our laboratory. Now it was applying successfully in allergy and immunology unit Ain Shams university hospitals in Egypt.
Cite this paper: M. Attia and H. Mohamed, "Assessment of Commercial Specific IgE Assay for Detection of Allergens in Allergic Patients," Journal of Analytical Sciences, Methods and Instrumentation, Vol. 3 No. 2, 2013, pp. 110-114. doi: 10.4236/jasmi.2013.32013.

[1]   R. Asero, G. Mistrello, R. Ariano, G. Colombo, M. E. Conte, M. Crivellaro, M. De Carli, F. D. Torre, F. Emiliani, F. L. Rizzini, R. Longo, D. Macchia, P. Minale, F. Murzilli, F. Nebiolo, O. Quercia, G. E. Senna and D. Villalta, “Shrimp Allergy in Italian Adults: A Multicenter Study Showing a High Prevalence of Sensitivity to Novel High Molecular Weight Allergens,” International Archives of Allergy and Immunology, Vol. 157, 2012, pp. 3-10. doi:10.1159/000324470

[2]   B. Manuel, I. Victor, M. Maria, H. Pinto, F. Carballada, C. Clara and C. Jeronimo, “Seafood Hypersensitivity in Mite Sensitized Individuals: Is Tropomyosin the Only Responsible Allergen?” Annals of Allergy, Vol. 106, No. 3, 2011, pp. 223-229.

[3]   A. J. Douglass and E. R. O’Hehir, “Diagnosis, Treatment and Prevention of Allergic Disease: The Basics,” Medical Journal Australia, Vol. 185, No. 4, 2006, pp. 228-233.

[4]   D. J. Hill, R. G. Heine and C. S. Hosking, “The Diagnostic Value of Skin Prick Testing in Children with Food Allergy,” Pediatric Allergy and Immunology, Vol. 15, No. 5, 2004, pp. 435-441. doi:10.1111/j.1399-3038.2004.00188.x

[5]   C. A. Yang, et al., “Measurement of IgE Antibodies to Shrimp Tropomyosin Is Superior to Skin Prick Testing with Commercial Extract and Measurement of IgE to Shrimp for Predicting Clinically Relevant Allergic Reactions after Shrimp Ingestion,” The Journal of Allergy and Clinical Immunology, Vol. 125, No. 4, 2010, pp. 872-878.

[6]   A. L. Lopata, R. E. O’Hehir and S. B. Lehrer, “Shellfish Allergy,” Clinical & Experimental Allergy, Vol. 40, No. 6, 2010, pp. 850-858. doi:10.1111/j.1365-2222.2010.03513.x

[7]   R. G. Slavin and R. E. Reisman, “Expert Guide to Allergy and Immunology,” Fulcrum Data Services, Philadelphia, 2001.

[8]   K. R. Krishnan, E. L. Peterson and G. Wegienka, “Pattern of Allergen-Specific IgE Sensitization Relative to Total Serum Ig Concentration in Young Adults,” Annals of Allergy, Asthma & Immunology, Vol. 105, No. 5, 2010, pp. 401-403. doi:10.1016/j.anai.2010.09.014

[9]   R. Hamilton, “Proficiency Survey-Based Evaluation of Clinical Total and Allergen-Specific IgE Assay Performance,” Archives of Pathology & Laboratory Medicine, Vol. 134, No. 7, 2010, pp. 975-982.

[10]   El-Shami and Aloba, “Liquid Phase in Vitro Allergen IgE Assay with Situimmobilization,” Advanced Biological Sciences, Vol. 74, 1989, pp. 191-201.

[11]   C. Garcia-Ara, J. Boyano-Wang, J. H. Godbold and H. A. Sampson, “Correlation of Serum Allergy (IgE) Tests Performed by Different Assay Systems,” The Journal of Allergy and Clinical Immunology, Vol. 121, No. 5, 2008, pp. 1219-1224. doi:10.1016/j.jaci.2007.12.1150

[12]   S. Celik-Bilgili, A. Mehl, A. Verstege, U. Staden, M. Nocon, K. Beyer, et al., “The Predictive Value of Specific Immunoglobulin E Levels in Serum for the Outcome of Oral Food Challenges,” Clinical & Experimental Allergy, Vol. 35, No. 3, 2005, pp. 268-273. doi:10.1111/j.1365-2222.2005.02150.x

[13]   J. M. Diaz-Pena, F. Martin-Munoz, M. Reche-Frutos and M. Martin-Esteban, “Specific IgE Levels in the Diagnosis of Immediate Hypersensitivity to Cows’ Milk Protein in the Infant,” The Journal of Allergy and Clinical Immunology, Vol. 107, No. 1, 2001, pp. 185-190. doi:10.1067/mai.2001.111592

[14]   R. G. Hamilton and A. N. Franklin, “In Vitro Assays for the Diagnosis of IgE-Mediated Disorders,” The Journal of Allergy and Clinical Immunology, Vol. 114, No. 2, 2004, pp. 213-225. doi:10.1016/j.jaci.2004.06.046