AJPS  Vol.4 No.5 , May 2013
Maternal Environment Effects on Phenolic Defenses in Abutilon theophrasti Seeds
Abstract: A class of phenolic compounds, ortho-dihydroxyphenols (hereafter “o-DHP”), has been implicated with seed survival. Based on expectations of the growth-differentiation balance hypothesis, we predicted that seed o-DHP concentration exhibits a curvilinear response to increasing resource availability in the maternal environment, with maximum o-DHP occurring at moderate resource levels. To test this hypothesis, Abutilon theophrasti seeds were produced under field conditions at two locations. Each location included twelve maternal environments established through factorial combinations of soil compost (+/-), species assemblage (A. theophrasti with and without maize), and soil nitrogen fertilizer (0, 0.5× or 1× local recommendations for maize). Resource availability with respect to A. theophrasti growth was summarized by above-ground biomass at seed harvest (maternal biomass). Results indicated that seed o-DHP concentrations increased then decreased in response to increasing maternal biomass. This relationship was modeled with a unimodal function specific to location (Location 1, y = 1.18 + 0.03xe-0.02x, pseudo-R2 = 0.59, p = 0.003; Location 2, y = 1.40 + 0.006xe-0.005x; pseudo-R2 = 0.34, p = 0.05). Seed protein concentrations remained constant across maternal biomass levels. Because inherent vulnerability to predation and decay is considered a consequence of chemical protection relative to nutritional offering, our results suggest that A. theophrasti seed susceptibility to lethal attack is influenced by resource levels in the maternal environment. More broadly, our results suggest that the growth-differentiation balance hypothesis can be extended to maternal effects on seed phenolics.
Cite this paper: B. Schutte, S. Wortman, J. Lindquist and A. Davis, "Maternal Environment Effects on Phenolic Defenses in Abutilon theophrasti Seeds," American Journal of Plant Sciences, Vol. 4 No. 5, 2013, pp. 1127-1133. doi: 10.4236/ajps.2013.45139.

[1]   M. Fenner and K. Thompson, “The Ecology of Seeds,” Cambridge University Press, Cambridge, 2005. doi:10.1017/CBO9780511614101

[2]   Y. Mohamed-Yasseen, S. A. Barringer, W. E. Splittstoesser and S. Costanza, “The Role of Seed Coats in Seed Viability,” The Botanical Review, Vol. 60, No. 4, 1994, pp. 427-439. doi:10.1007/BF02857926

[3]   R. T. Wilkens, J. M. Spoerke and N. E. Stamp, “Differential Responses of Growth and Two Soluble Phenolics of Tomato to Resource Availability,” Ecology, Vol. 77, No. 1, 1996, pp. 247-258. doi:10.2307/2265674

[4]   C. Glynn, D. A. Herms, C. M. Orians, R. C. Hansen and S. Larsson, “Testing the Growth-Differentiation Balance Hypothesis: Dynamic Responses of Willows to Nutrient Availability,” New Phytologist, Vol. 176, No. 3, 2007, pp. 623-634. doi:10.1111/j.1469-8137.2007.02203.x

[5]   J. Le Bot, C. Benard, C. Robin, F. Bourgaud and S. Adamowicz, “The ‘Trade-Off’ between Synthesis of Primary and Secondary Compounds in Young Tomato Leaves Is Altered by Nitrate Nutrition: Experimental Evidence and Model Consistency,” Journal of Experimental Botany, Vol 60, No. 15, 2009, pp. 4301-4314. doi:10.1093/jxb/erp271

[6]   W. F. Loomis, “Growth-Differentiation Balance vs Carbohydrate-Nitrogen Ratio,” Proceedings of the American Society for Horticultural Science, Vol. 29, 1932, pp. 240- 245.

[7]   D. A. Herms and W. J. Mattson, “The Dilemma of Plants: To Grow or Defend,” Quarterly Review of Biology, Vol. 67, No. 3, 1992, pp. 283-335. doi:10.1086/417659

[8]   G. F. Hendry, K. Thompson, C. J. Moss, E. Edwards and P. C. Thorpe, “Seed Persistence: A Correlation between Seed Longevity in the Soil and Ortho-Dihydroxyphenol Concentration,” Functional Ecology, Vol. 8, No. 5, 1994, pp. 658-664. doi:10.2307/2389929

[9]   R. L. Nicholson and R. Hammerschmidt, “Phenolic Compounds and Their Role in Disease Resistance,” Annual Review of Phytopathology, Vol. 30, 1992, pp. 369-389. doi:10.1146/

[10]   D. L. Clark and M. V. Wilson, “Post-Dispersal Seed Fates of Four Prairie Species,” American Journal of Botany, Vol. 90, No. 5, 2003, pp. 730-735. doi:10.3732/ajb.90.5.730

[11]   S. I. Warwick and L. D. Black, “The Biology of Canadian Weeds: Abutilon theophrasti,” Canadian Journal of Plant Science, Vol. 68, No. 4, 1988, pp. 1069-1085. doi:10.4141/cjps88-127

[12]   R. E. Nurse and A. Ditommaso, “Corn Competition Alters the Germinability of Velvetleaf (Abutilon theophrasti) Seeds,” Weed Science, Vol. 53, No. 4, 2005, pp. 479-488. doi:10.1614/WS-04-185R1

[13]   J. A. D. Parrish and F. A. Bazzaz, “Nutrient content of Abutilon theophrasti seeds and the competitive ability of the resulting plants,” Oecologia, Vol. 65, No. 2, 1985, pp. 247-251. doi:10.1007/BF00379224

[14]   B. J. Schutte, A. S. Davis, K. A. Renner and J. Cardina, “Maternal and Burial Environment Effects on Seed Mortality of Velvetleaf (Abutilon theophrasti) and Giant Foxtail (Setaria faberi),” Weed Science, Vol. 56, No. 6, 2008, pp. 834-840. doi:10.1614/WS-08-031.1

[15]   F. R. Earle and Q. Jones, “Analyses of Seed Samples from 113 Plant Families,” Economic Botany, Vol. 16, No. 4, 1962, pp. 221-250. doi:10.1007/BF02860181

[16]   G. M. Dugan and M. R. Gumbmann, “Toxicological and Nutritional Evaluation of Velvetleaf Seed: Subchronic 90-Day Feeding Study and Protein Efficiency Ratio Assay,” Food and Chemical Toxicology, Vol. 28, No. 2, 1990, pp. 95-99. doi:10.1016/0278-6915(90)90016-G

[17]   G. F. Hendry, “Defense Chemistry (Phenols),” In: G. F. Hendry and J.P. Grime, Eds., Methods in Comparative Plant Ecology: A Laboratory Manual, Chapman & Hall, London, 1993, pp. 180-181. doi:10.1007/978-94-011-1494-3

[18]   R. L. Mulvaney, S. A. Khan and T. R. Ellsworth, “Need for a Soil-Based Approach in Managing Nitrogen Fertilizers for Profitable Corn Production,” Soil Science Society of America Journal, Vol. 70, No. 1, 2006, pp. 172-182. doi:10.2136/sssaj2005.0034

[19]   A. S. Davis, B. J. Schutte, J. Iannuzzi and K. A. Renner, “Chemical and Physical Defense of Weed Seeds in Relation to Soil Seedbank Persistence,” Weed Science, Vol. 56, No. 5, 2008, pp. 676-684. doi:10.1614/WS-07-196.1

[20]   G. F. Hendry and P. C. Thorpe, “Organic Reserves,” In: G. F. Hendry and J. P. Grime, Eds., Methods in Comparative Plant Ecology: A Laboratory Manual, Chapman & Hall, London, 1993, pp. 196-199. doi:10.1007/978-94-011-1494-3

[21]   R. J. Carroll, D. Ruppert, L. A. Stefanski and C. M. Crainiceanu, “Measurement Error in Nonlinear Models: A Modern Perspective,” 2nd Edition, Chapman & Hall/CRC, New York, 2006. doi:10.1201/9781420010138

[22]   O. Schabenberger and F. J. Pierce, “Contemporary Statistical Models for the Plant and Soil Sciences,” CRC Press, New York, 2002.

[23]   J. H. Zar, “Biostatistical Analysis,” 4th Edition, Prentice Hall, Upper Saddle River, 1999.

[24]   Y. Gutterman, “Maternal Effects on Seeds during Development,” In: M. Fenner, Ed., Seeds: The Ecology of Regeneration in Plant Communities, 2nd Edition, CAB International, New York, 2000, pp. 59-84. doi:10.1079/9780851994321.0059

[25]   A. S. Steinbrenner, N. Agerbirk, C. M. Orians and F. S. Chew, “Transient Abiotic Stresses Lead to Latent Defense and Reproductive Responses over the Brassica Rapa Life Cycle,” Chemoecology, Vol. 22, No. 4, 2012, pp. 239- 250 doi:10.1007/s00049-012-0113-y

[26]   D. Kestring, L. R. Menezes, C. A. Tomaz, G. P. Lima and M. N. Rossi, “Relationship among Phenolic Contents, Seed Predation, and Physical Seed Traits in Mimosa bimucronata Plants,” Journal of Plant Biology, Vol. 52, No. 6, 2009, pp. 569-576. doi:10.1007/s12374-009-9073-3

[27]   W. Otten and C. A. Gilligan, “Soil Structure and Soil- Borne Diseases: Using Epidemiological Concepts to Scale from Fungal Spread to Plant Epidemics,” European Journal of Soil Science, Vol. 57, No. 1, 2006, pp. 26-37. doi:10.1111/j.1365-2389.2006.00766.x

[28]   K. Donohue and J. Schmitt, “Maternal Environmental Effects in Plants: Adaptive Plasticity?” In: T. A. Mousseau and C. W. Fox, Eds., Maternal Effects as Adaptations, Oxford University Press, New York, 1998, pp. 137-158.

[29]   R. J. Kremer, L. B. Hughes and R. J. Aldrich, “Examination of Microorganisms and Deterioration Resistance Mechanisms Associated with Velvetleaf Seed,” Agronomy Journal, Vol. 76, No. 5, 1984, pp. 745-749. doi:10.2134/agronj1984.00021962007600050009x

[30]   J. W. Dalling, A. S. Davis, B. J. Schutte and A. E. Arnold, “Seed Survival in Soil: Interacting Effects of Predation, Dormancy and the Soil Microbial Community,” Journal of Ecology, Vol. 99, No. 1, 2001, pp. 89-95. doi:10.1111/j.1365-2745.2010.01739.x