Chang Dong, Meng Zhang, Zhiying Yu, Junpeng Ren, Yang Qin, Bailin Wang, Lizhen Xiao, Zhen Zhang, Jianmin Tao

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College of Horticulture, Nanjing Agricultural University, Nanjing, China.
Department of Horticulture, Heilongjiang Academy of Agricultural Science, Harbin, China.
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Abstract: The C-repeat Binding Factor/Dehydration Responsive Element Binding factor (CBF/DREB) responsive pathway is involved in plant response to abiotic stress. In this study, we report the isolation of VaCBF4 (a complete cDNA) and its promoter from Vitis amurensis through rapid amplification of cDNA ends and genome walking techniques, respectively. The CBF4 transcript accumulation of V. amurensis increased under cold, salinity, and abscisic acid (ABA) and salicylic acid (SA) treatments, whereas that of Vitis vinifera showed a different change. The transcript levels of VaCBF4 inthe roots, stems, leaves, and petioles under cold, salinity, and ABA and SA treatments were up-regulated in a timedependent manner. The presence of the cis-elements MBC, ABRE, and as-2-box in the VaCBF4 promoter further confirmed that this promoter is a component of the CBF transduction pathway, which is involved in plant response to multistress.

Keywords: Vitis amurensis; CBF4; Stress; Transcript Accumulation

Cite this paper: Dong, C. , Zhang, M. , Yu, Z. , Ren, J. , Qin, Y. , Wang, B. , Xiao, L. , Zhang, Z. and Tao, J. (2013) Isolation and expression analysis of CBF4 from Vitis amurensis associated with stress. Agricultural Sciences, 4, 224-229. doi: 10.4236/as.2013.45032.

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