The C-repeat Binding
Factor/Dehydration Responsive Element Binding factor (CBF/DREB) responsive pathway is involved in plant response to abiotic stress. In this study, we report the isolation of VaCBF4 (a
complete cDNA) and its promoter from Vitis
amurensis through rapid amplification of cDNA ends and genome walking techniques, respectively. The CBF4 transcript
accumulation of V. amurensis increased
under cold, salinity, and abscisic acid (ABA) and salicylic acid (SA)
treatments, whereas that of Vitis vinifera showed a different change. The transcript levels of VaCBF4 inthe roots, stems, leaves, and petioles
under cold, salinity, and ABA and SA treatments were up-regulated in a timedependent
manner. The presence of the cis-elements
MBC, ABRE, and as-2-box in the VaCBF4 promoter further confirmed that this
promoter is a component of the CBF transduction pathway, which is involved in
plant response to multistress.
Cite this paper
Dong, C. , Zhang, M. , Yu, Z. , Ren, J. , Qin, Y. , Wang, B. , Xiao, L. , Zhang, Z. and Tao, J. (2013) Isolation and expression analysis of CBF4 from Vitis amurensis associated with stress. Agricultural Sciences, 4, 224-229. doi: 10.4236/as.2013.45032.
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