AJAC  Vol.4 No.5 , May 2013
Simultaneous Quantification of Carbohydrates, Alcohols, and Toxic Components in a Bio-Based Medium Using Dual-Detection HPLC Analysis
Abstract: Bio-based media, derived from cellulosic biomass depolymerisation or bioconversion processes, are composed of several chemicals and biochemicals. In this study, the main components of a hemicellulosic-based medium were analyzed using a dual detection HPLC method to separate and determine concentrations of the major monosaccharides (glucose, xylose, and arabinose), alcoholic and acidic components (ethanol, xylitol, and acetic acid) and furanic compounds (furfural and hydroxymethylfurfural (HMF)). The analyses, which employed a single stationary phase (Aminex HPX-87H) and two detection methods, were carried out under isocratic conditions and involved mobile phases consisting of 5 mM sulfuric acid and acetonitrile in different mix ratios from 0 to 0.061 mole fraction of acetonitrile. Based on the analysis run time and the chromatogram quality, the optimum condition was determined for the simultaneous quantification of the components.
Cite this paper: M. Soleimani and L. Tabil, "Simultaneous Quantification of Carbohydrates, Alcohols, and Toxic Components in a Bio-Based Medium Using Dual-Detection HPLC Analysis," American Journal of Analytical Chemistry, Vol. 4 No. 5, 2013, pp. 265-272. doi: 10.4236/ajac.2013.45033.

[1]   A. Corma, S. Iborra and A. Velty, “Chemical Routes for the Transformation of Biomass into Chemicals,” Chemical Reviews, Vol. 107, No. 6, 2007, pp. 2411-2502. doi:10.1021/cr050989d

[2]   E. Palmqvist and B. Hagerdal, “Fermentation of Lignocellulosic Hydrolysates. II: INHIBITORS and Mechanisms of Inhibition,” Bioresource Technology, Vol. 74, No. 1, 2000, pp. 25-33. doi:10.1016/S0960-8524(99)00161-3

[3]   X. Chen, Z. H. Jiang, S. Chen and W. Qin, “Microbial and Bioconversion Production of Xylitol and Its Detection and Application,” International Journal of Biological Sciences, Vol. 6, No. 7, 2010, pp. 834-844. doi:10.7150/ijbs.6.834

[4]   X. Liu, N. Ai, H. Zhang, M. Lu, D. Ji, F. Yu and J. Ji, “Quantification of Glucose, Xylose, Arabinose, Furfural and HMF in Corncob Hydrolysate by HPLC-PDA-ELSD,” Carbohydrate Research, Vol. 353, 2012, pp. 111-114. doi:10.1016/j.carres.2012.03.029

[5]   A. Martinez, M. E. Rodriguez, S. W. York, J. F. Preston and L. O. Ingram, “Use of UV Absorbance to Monitor Furans in Dilute Acid Hydrolysates of Biomass,” Biotechnology Progress, Vol. 16, No. 4, 2000, pp. 637-641. doi:10.1021/bp0000508

[6]   S. M. Rocha, M. A. Coimbra and I. Delgadillo, “Occurrence of Furfuraldehydes during the Processing of Quercus suber L. cork. Simultaneous Determination of Furfural, 5-Hydroxymethylfurfural and 5-Methylfurfural and Their Relation with Cork Polysaccharides,” Carbohydrate Polymers, Vol. 56, No. 3, 2004, pp. 287-293. doi:10.1016/j.carbpol.2004.03.002

[7]   C. J. Scarlata and D. A. Hyman, “Development and Validation of a Fast High Pressure Liquid Chromatography for the Analysis of Lignocellulosic Biomass Hydrolysis and Fermentation Products,” Journal of Chromatograpghy A, Vol. 1217, No. 14, 2010, pp. 2082-2087. doi:10.1016/j.chroma.2010.01.061

[8]   H. Xu, A. C. Templeton and R. A. Reed, “Quantification of 5-HMF and Dextrose in Commercial Aqueous Dextrose Solutions,” Journal of Pharmaceutical and Biomedical Analysis, Vol. 32, No. 3, 2003, pp. 451-459. doi:10.1016/S0731-7085(03)00163-8

[9]   A. S. Dias, S. Lima, M. Pillinger and A. A. Valente, “Acidic Cesium Salts of 12-Tungstophosphoric Acid as Catalysts for the Dehydration of Xylose into Furfural,” Carbohydrate Research, Vol. 341, No. 18, 2006, pp. 2946-2953. doi:10.1016/j.carres.2006.10.013

[10]   K. Hickey and W. E. Waghorne, “Viscosities and Volumes of Dilute Solutions of Formamide in Water + Acetonitrile and for Formamide and N,N-Dimethylformamide in Methanol + Acetonitrile Mixed Solvents:" Viscosity BCoefficients, Activation Free Energies for Viscous Flow, and Partial Molar Volumes,” Journal of Chemical and Engineering Data, Vol. 46, No. 4, 2001, pp. 851-857. doi:10.1021/je0003647

[11]   T. Aburjai, M. Alzweiri and Y. M. Al-Hiari, “Temperature and Pressure Behaviours of Methanol, Acetonitrile/ Water Mixtures on Chromatographic Systems,” American Journal of Analytical Chemistry, Vol. 2, No. 8, 2011, pp. 934-937. doi:10.4236/ajac.2011.28108

[12]   J. P. Yuan and F. Chen, “Simultaneous Separation and Determination of Sugars, Ascorbic Acid and Furanic Compounds by HPLC-Dual Detection,” Food Chemistry, Vol. 64, No. 3, 1999, pp. 423-427. doi:10.1016/S0308-8146(98)00091-0

[13]   H. A. Eyeghe-Bickong, E. O. Alexandersson, L. M. Gouws, P. R. Young and M. A. Vivier, “Optimisation of an HPLC Method for the Simultaneous Quantification of the Major Sugars and Organic Acids in Grapevine Berries,” Journal of Chromatography B, Vol. 885-886, 2012, pp. 43-49. doi:10.1016/j.jchromb.2011.12.011