Back
 AJAC  Vol.4 No.5 , May 2013
Determination of Ketoprofen in Human Plasma by RP-HPLC
Abstract: For in vivo pharmacokinetic studies, it is pre-requisite to quantify drug concentrations in plasma. In the present study a RP-HPLC procedure was developed and validated for the assessment of ketoprofen in human plasma. For this purpose mobile phase consisting of methaol:water (70:30) adjusted to pH 3.3 with phosphoric acid was used, and chromatography was carried out on Discovery HS C18 column, 5 μm (25 cm × 4.6 mm). The flow rate was 1 mL·min-1 and quantitative assessment was performed at 260 nm. The retention time was found to be <10 min. A method was found to be accurate and illustrated linearity from 0.2441 to 125 μg·mL-1 with the determination coefficient (r2) of 0.9999, also accuracy and precision were found to be <2 (%RSD). The intraday accuracy for concentrations 62.5 μg·mL-1, 15.625 μg·mL-1, 7.812 μg·mL-1 and 1.953 μg·mL-1 were found to be 99.747%, 99.475%, 98.457% and 99.824% respectively where as for interday accuracy consecutive values for days 1, 2 and 3 were 99.104%, 99.091%, 98.96% and 99.385% in plasma. All validation parameters were assessed and were found to be within the limits. The proposed method was accurate, specific, quick (retention time < 10 min), selective (showed no interference with excipients), cost effective and a good resolution which gave this method an advantage over the different other reported methods for the estimation of ketoprofen in human plasma.
Cite this paper: F. Zafar, M. Shoaib, A. Naz, R. Yousuf and H. Ali, "Determination of Ketoprofen in Human Plasma by RP-HPLC," American Journal of Analytical Chemistry, Vol. 4 No. 5, 2013, pp. 252-257. doi: 10.4236/ajac.2013.45031.
References

[1]   S. C. Dollery, “Therapeutic Drugs,” Churchill Livingstone, Philadelphia, 1991, pp. 25-26.

[2]   J. Fossgreen, “Ketoprofen: A Survey of Current Publications,” Scandinavian Journal of Rheumatology, Vol. 5, Suppl. 14, 1976, pp. 7-32.

[3]   J. R. Caldwell, B. F. Germain and S. H. Lourie, “Ketoprofen versus Indomethacin in Patients with Rheumatoid Arthritis: A Multicenter Double Blind Comparative Study,” Journal of Rheumatology, Vol. 15, No. 10, 1988, pp. 1476-1479.

[4]   N. Sultana, M. S. Arayne and S. Naveed, “Simultaneous Quantitation of Captopril and NSAID’s in API, Dosage Formulations and Human Serum by RP-HPLC,” Journal of the Chinese Chemical Society, Vol. 57, No. 1, 2010, pp. 62-67.

[5]   H. Y. Aboul-Enein, A. G. Dal and M. Tuncel, “A Validated Method Development for Ketoprofen by a Flow-Injection Analysis with UV-Detection and Its Application to Pharmaceutical Formulations,” Farmaco, Vol. 58, No. 6, 2003, pp. 419-422. doi:10.1016/S0014-827X(03)00061-2

[6]   K. E. Pickla, C. Magnesa, M. Bodenlenza, T. R. Piebera and F. M. Sinner, “Rapid Online-SPE-MS/MS Method for Ketoprofen Determination in Dermal Interstitial Fluid Samples from Rats Obtained by Microdialysis or Open-Flow Microperfusion,” Journal of Chromatography B, Vol. 850, No. 1-2, 2007, pp. 432-439. doi:10.1016/j.jchromb.2006.12.026

[7]   F. Zafar, M. H. Shoaib and R. I. Yousuf, “Development and Evaluation of Fast Dispersible Ketoprofen 100 mg Tablets,” Asian Journal of Pharmaceutical Research, Vol. 2, No. 1, 2012, pp. 1-9.

[8]   “International Conference on the Harmonization of Technical Requirements for the Registration of Pharmaceuticals for Human Use (ICH) Q2B,” International Journal of Pharmaceutical and Life Sciences, Vol. 1, No. 3, 1996, pp. 127-132.

[9]   A. S. Lister, “Validation of HPLC Methods in Pharmaceutical Analysis,” Elsevier Inc., UK, 2005, pp. 197-211.

[10]   Y. Hassan and A. Enein, “Rapid Liquid Chromatographic Analysis of Metoclopramide in Pharmaceutical Preparations,” Toxicological & Environmental Chemistry, Vol. 26, No. 1-4, 1990, pp. 197-201. doi:10.1080/02772249009357546

[11]   N. Sultana, M. S. Arayne, R. Siddiqui and S. Naveed, “RP-HPLC Method for the Simultaneous Determination of Lisinopril and NSAIDs in API, Pharmaceutical Formulations and Human Serum,” American Journal of Analytical Chemistry, Vol. 3, No. 2, 2012, pp. 147-152. doi:10.4236/ajac.2012.32021

 
 
Top