Collagen types in relation to expression of estradiol and progesterone receptors in equine endometrial fibrosis
Author(s)
Diego Lunelli,
Silvana Maris Cirio,
Selene C. Leite,
Carlos Eduardo Camargo,
Luiz Ernandes Kozicki*
Affiliation(s)
Pontifical Catholic University of Paraná, Paraná, Brazil. Veterinary Medicine College, University Centrum of Evangelica, Curitiba, Brazil. Federal University of Paraná, Paraná, Brazil. Veterinary Medicine College, Pontifical Catholic University of Paraná, Paraná, Brazil.
Pontifical Catholic University of Paraná, Paraná, Brazil. Veterinary Medicine College, University Centrum of Evangelica, Curitiba, Brazil. Federal University of Paraná, Paraná, Brazil. Veterinary Medicine College, Pontifical Catholic University of Paraná, Paraná, Brazil.
ABSTRACT
The aim of this study was to determine the influence of collagen I and III on the expression of estrogen and progesterone receptors in equine endometrial fibrosis. A total of 25 crossbred mares were studies. Two endometrial samples were collected from each mare,1 inthe estrus and1 inthe diestrus phase. The samples were classified according to histological changes. Collagen was typed and quantified using the picrosirius red histochemical technique, and steroid receptors were identified by immunohistochemistry. The results showed a predominance of collagen type III in all the endometrial samples. The expression of estrogen (RE2) and progesterone (RP4) receptors varied according to the estrous cycle. RE2 and RP4 expression varied in the estrus and diestrus phases; there was no influence of collagen I or II on receptor expression.
Cite this paper
Lunelli, D. , Cirio, S. , Leite, S. , Camargo, C. and Kozicki, L. (2013) Collagen types in relation to expression of estradiol and progesterone receptors in equine endometrial fibrosis. Advances in Bioscience and Biotechnology, 4, 599-605. doi: 10.4236/abb.2013.44078.
Lunelli, D. , Cirio, S. , Leite, S. , Camargo, C. and Kozicki, L. (2013) Collagen types in relation to expression of estradiol and progesterone receptors in equine endometrial fibrosis. Advances in Bioscience and Biotechnology, 4, 599-605. doi: 10.4236/abb.2013.44078.
References
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(2009) Caracterização imunoistoquímica dos miofibroblastos endometriais e da expressão de MMP-2 nas endometrites crônicas das éguas. Dissertação (mestrado)—Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu. [23] Walter, I., Handler, J., Reifinger, M. and Aurich, C. (2001) Association of endometrosis in horses with differentiation of periglandular myofibroblasts and changes of extracellular matrix proteins. Reproduction, 121, 581-586. doi:10.1530/rep.0.1210581 [24] Watson, E.D., Skolnik, S.B. and Zanecosky, H.G. (1992) Progesterone and estrogen receptor distribution in the endometrium of the mare. Theriogenology, 38, 575-580. doi:10.1016/0093-691X(92)90020-R [25] Clark, J.H., Scrader, W.T. and O’Malley, B.W. (1985) Mechanisms of steroid hormone action. In: Witson, J.D. and Fortec, D.W., Eds., Textbook of Endocrinology, W.B. Saunders, Philadelphia, 33-75. [26] Tomanelli, R.N., Sertich, P.L. and Watson, E.D. (1991) Concentrations of oestrogen and progesterone receptor in the endometrium of the mare. Journal of Reproduction & Fertility, 44, 267-273. [27] Ricketts, S.W. and Alonso, S. (1991) The effect of age and parity on the development of equine chronic endometrial disease. Equine Veterinary Journal, 23, 189-192. doi:10.1111/j.2042-3306.1991.tb02752.x
[1] Kenney, R.M. (1978) Cyclic and pathologic changes of the mare endometrium as detected by biopsy, with a note on embryonic death. Journal of the American Veterinary Medical Association, 172, 241-262. [2] Gray, C.A., Bartol, F.F., Tarleton, B.J., Wiley, A.A., Johnson, G.A., Bazer, F.W. and Spencer, T.E. (2001) Developmental biology of uterine glands. Biological Reproduction, 65, 1311-1323. doi:10.1095/biolreprod65.5.1311 [3] Masseno, A.P.B. (2012) Avaliação da fibrose endometrial e dos miofibroblastos na endometroses ativa e inativa das éguas. Tese (doutorado)—Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, 2012. [4] Guyot, C., Lepreux, S., Combe, C., Doudnikoff, E., Bioulac-Sage, P., Balabaud, C. and Desmouliére, A. (2006) Hepatic fibrosis and cirrhosis: The (myo)fibroblastic cell subpopulations involved. International Journal of Biochemistry and Cell Biology, 38, 135-151. [5] Martinez-Hernandez, A. (1999) Repair, regeneration and fibrosis. In: Rubin, E. and Faber, J.L., Eds., Pathology, 3rd Edition, Lippincot-Raven, Philadelphia. [6] Bochsler, P.N. and Slauson, D.O. (2002) Inflammation and repair of tissue. In: Slauson, D.O. and Cooper, B.J., Eds., Mechanisms of Disease: A Textbook of Comparative General Pathology, 3rd Edition, Mosby, St. Louis, 140-245. [7] Junqueira, L.C.U., Cossermelli, W. and Brentani, R.R. (1978) Diferencial staining of collagens type I, II and III by Sirius Red and polarization microscopy. Archives of Histology of Japan, 41, 267-274. doi:10.1679/aohc1950.41.267 [8] Montes, G.S. and Junqueira, L.C.U. (1991) The use of the picrosirius-polarization method for the study of the biopathology of collagen. Memórias do Instituto Oswaldo Cruz, 86, 111. doi:10.1590/S0074-02761991000700002 [9] Borges, L.F., Gutierrez, P.S., Marana, H.R.C. and Taboga, S.R. (2007) Picrosirius-polarization staining method as an efficient histopatho-logical tool for collagenolysis detection in vesical prolapsed lesions. Micron, 38, 580-583. doi:10.1016/j.micron.2006.10.005 [10] Schlafer, D.H. (2007) Equine endometrial biopsy: Enhancement of clinical value by more extensive histopathology and application of new diagnostic techniques? Theriogenology, 68, 413-422. doi:10.1016/j.theriogenology.2007.04.040 [11] Nunes, L.C. (2003) Avaliação histopatológica, histoquímica, imunohistoquímica e morfométrica das endometrites crônicas em éguas. Disserta#231;ão (mestrado)—Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu. [12] Hoffmann, C., Ellenberger, C., Mattos, R.C., Aupperle, H., Dhein, S., Stief, B. and Schoon, H.A. (2009) The equine endometrosis: New insights into the pathogenesis. Animal Reproduction Science, 111, 261-278. doi:10.1016/j.anireprosci.2008.03.019 [13] Aupperle, H., Ozgen Schoon, H.A., Schoon, D., Hoppen, H.O., Sieme, H. and Tannapfel, A. (2000) Cyclical endometrial steroid hormone receptor expression and proliferation intensity in the mare. Equine Veterinary Journal, 32, 228-232. doi:10.2746/042516400776563554 [14] Kenney, R.M. and Doig, P.A. (1986) Equine endometrial biopsy. In: Morrow, D.A., Ed., Current Therapy in Theriogenology, W.B. Saunders, Philadelphia, 723-729. [15] Porto, C.D. (2009) Expressão de TGF-β1, metaloproteinases e avaliação dos índices de proliferação celular e apoptótico nas endometrites crônicas das éguas. Tese (doutorado)—Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu. [16] Kenney, R.M. (1975) Prognostic value of endometrial biopsy of the mare. Journal of Reproduction and Fertility, 23, 347-348. [17] Bergman, R.V. and Kenney, R.M. (1975) Representativeness of a uterine biopsy in the mare. Proceedings AAEP, 355-361. [18] Waelchli, R.O. and Winder, N.C. (1989) Distribution of histological lesions in the equine endometrium. Veterinary Record, 124, 274-276. doi:10.1136/vr.124.11.271 [19] Keller, A., Neves, A.P., Aupperle, H., Steiger, K., Garbade, P., Schoon, H.A., Klug, E. and Mattos, R.C. (2006) Repetitive experimental bacterial infections do not affect the degree of uterine degeneration in the mare. Animal Reproduction Science, 94, 276-279. doi:10.1016/j.anireprosci.2006.04.012 [20] Fiala, S., Esmeraldino, A.M.T., Jobim, M.I.M., Garbade, P., Wolf, C.A., Richter, G., Gregory, R.M. and Mattos, R.C. (2010) Endometrial fibrotic changes. Is one biopsy enough to diagnose degenerative changes? Animal Reproduction Science, 121, 89-90. doi:10.1016/j.anireprosci.2010.04.133 [21] Porto, C.D. (2006) Caracteriza#231;ão histoquímica do colágeno e expressão de MMP-2, MMP-9 e TIMP-1 nas endometrites crônicas das éguas. Disserta#231;ão (mestrado)— Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu. [22] Masseno, A.P.B. (2009) Caracterização imunoistoquímica dos miofibroblastos endometriais e da expressão de MMP-2 nas endometrites crônicas das éguas. Dissertação (mestrado)—Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu. [23] Walter, I., Handler, J., Reifinger, M. and Aurich, C. (2001) Association of endometrosis in horses with differentiation of periglandular myofibroblasts and changes of extracellular matrix proteins. Reproduction, 121, 581-586. doi:10.1530/rep.0.1210581 [24] Watson, E.D., Skolnik, S.B. and Zanecosky, H.G. (1992) Progesterone and estrogen receptor distribution in the endometrium of the mare. Theriogenology, 38, 575-580. doi:10.1016/0093-691X(92)90020-R [25] Clark, J.H., Scrader, W.T. and O’Malley, B.W. (1985) Mechanisms of steroid hormone action. In: Witson, J.D. and Fortec, D.W., Eds., Textbook of Endocrinology, W.B. Saunders, Philadelphia, 33-75. [26] Tomanelli, R.N., Sertich, P.L. and Watson, E.D. (1991) Concentrations of oestrogen and progesterone receptor in the endometrium of the mare. Journal of Reproduction & Fertility, 44, 267-273. [27] Ricketts, S.W. and Alonso, S. (1991) The effect of age and parity on the development of equine chronic endometrial disease. Equine Veterinary Journal, 23, 189-192. doi:10.1111/j.2042-3306.1991.tb02752.x