(Bombyx mori) is a species of
agricultural importance, as well as a model organism for Lepidoptera insects.
Proteomic method has been widely used in silkworm research, and a robust mass
spectrometry-compatible protein extraction method is urgently needed. In
this study, we adapted phenol extraction method to extract silkworm midgut protein,
and coupled this method with pH 5 - 8 gel strip for two dimensional electrophoresis.
The phenol extraction method significantly increased the resolution, as well
as greatly reduced the background of two dimensional electrophoresis gels. In
addition, this method was well compatible with mass spectrometry analysis. This
is the first report that phenol extraction method is used for silkworm midgut
protein extraction, and may be applied in other researches.
Cite this paper
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