JBNB  Vol.4 No.2 , April 2013
Study of Human Serum Albumin Adsorption and Conformational Change on DLC and Silicon Doped DLC Using XPS and FTIR Spectroscopy
ABSTRACT

Diamond-like carbon (DLC) coatings are extremely useful for creating biocompatible surfaces on medical implants. DLC and silicon doped DLC synthesised on silicon wafer substrate by using plasma enhanced chemical vapour deposition (PECVD). The effects of surface morphology on the interaction of HSA with doped and undoped DLC films have been investigated. The chemical composition of the surface before and after adsorption was analysed using X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR). Results showed that silicon incorporation DLC tends to increase of sp3/sp2 hybridization ratio by decreasing sp2 hybridized carbon bonding configurations. Following exposure to solutions containing (0.250 μg/ml) HSA, the results indicated that significant changes in the C, N and O levels on the surfaces with reducing of the Si2p band at 100 eV. From FTIR spectrum, the peaks occur the following functional groups were assigned as amide I and II groups at 1650 cm-1 and 1580 cm-1. Both XPS and FTIR spectroscopy confirm that HSA was bound onto the surfaces of the DLC and Si-DLC films via interaction of ionized carboxyl groups and the amino group did not play a significant role in the adsorption of protein. These results from peak intensity show that an adsorbed layer of HSA is higher at high level (19%) silicon doping. Therefore doping of DLC may provide an approach to controlling the protein adsorption.


Cite this paper
M. Ahmed, J. Byrne, J. McLaughlin and W. Ahmed, "Study of Human Serum Albumin Adsorption and Conformational Change on DLC and Silicon Doped DLC Using XPS and FTIR Spectroscopy," Journal of Biomaterials and Nanobiotechnology, Vol. 4 No. 2, 2013, pp. 194-203. doi: 10.4236/jbnb.2013.42024.
References
[1]   C. M. Alves, R. L. Reis and J. A. Hunt, “The Competitive Adsorption of Human Proteins onto Natural-Based Biomaterials,” Journal of the Royal Society Interface, Vol. 7, No. 50, 2010, pp. 1367-1377. doi:10.1098/rsif.2010.0022

[2]   T. Hasebe, A. Hotta, H. Kodama, A. Kamijo, K. Takahashi and T. Suzuki, “Recent Advances in Diamond-Like Carbon Films in the Medical and Food Packing Fields,” New Diamond and Fornier Carbon Technology, Vol. 17, No. 6, 2007, pp. 263-279.

[3]   H. W. Choi, R. H. Dauskardt, S. C. Lee, K. R. Lee and K. H. Oh, “Characteristic of Silver Doped DLC Films on Surface Properties and Protein Adsorption,” Diamond & Related Materials, Vol. 17, No. 3, 2008, pp. 252-257. doi:10.1016/j.diamond.2007.12.034

[4]   D. Grimanelis, S. Yang, O. Bohme, E. Roman, A. Alberdi, D. G. Teer and J. M. Albella, “Carbon Based Coating for High Temperature Cutting Tools,” Applications, Diamond and Related Materials, Vol. 11, No. 2, 2002, pp. 176-184. doi:10.1016/S0925-9635(01)00566-0

[5]   J. I. Onate, M. Comin, I. Braceras, A. Garcia, J. L. Viviente, M. Brizuela, N. Garagorri, J. L. Perisb and J. I. Alava, “Wear Reduction Effect on Ultra-High-MolecularWeight Polyethylene by Application of Hard Coatings and Ion Implantation on Cobalt Chromium Alloy, as Measured in a Knee Wear Simulation Machine,” Surface Coatings Technology, Vol. 142-144, 2001, pp. 1056-1062. doi:10.1016/S0257-8972(01)01074-X

[6]   A. Alanazi, C. Nojiri, T. Kido, T. Noguchi, Y. Ohgoe, T. Matsuda, K. Hirakuri, A. Funakubo, K. Sakai and Y. Fukui, “Engineering Analysis of Diamond-Like Carbon Coated Polymeric Materials for Biomedical Applications,” Artificial Organs, Vol. 24, No. 8, 2000, pp. 624-627. doi:10.1046/j.1525-1594.2000.06576.x

[7]   R. K. Roy, H. W. Choi, J. W. Yi, M. W. Moon, K. R. Lee, D. K. Han, J. H. Shin, A. Kamijo and T. Hasebe, “Hemocompatibility of Surface-Modified, Silicon-Incorporated, Diamond-Like Carbon Films,” Acta Biomaterialia, Vol. 5, No. 1, 2009, Article ID: 249256. doi:10.1016/j.actbio.2008.07.031

[8]   S. C. H. Kwok, W. Zhang, G. J. Wan, D. R. McKenzie, M. M. M. Bilek and P. K. Chu, “Hemocompatibility and Anti-Bacterial Properties of Silver Doped Diamond-Like Carbon Prepared by Pulsed Filtered Cathodic Vacuum Arc Deposition,” Diamond & Related Materials, Vol. 16, No. 4-7, 2007, pp. 1353-1360. doi:10.1016/j.diamond.2006.11.001

[9]   M. H. Ahmed and J. A. Byrne, “Effect of Surface Structure and Wettability of DLC and N-DLC Thin Films on Adsorption of Glycine,” Applied Surface Science, Vol. 258, No. 12, 2012, pp. 5166-5174. doi:10.1016/j.apsusc.2012.01.162

[10]   M. H. Ahmed, J. A. Byrne and J. McLaughlin, “Evaluation of Glycine Adsorption on Diamond Like Carbon (DLC) and Fluorinated DLC Deposited by Plasma-Enhanced Chemical Vapour Deposition (PECVD),” Surface & Coatings Technology, Vol. 209, 2012, pp. 8-14. doi:10.1016/j.surfcoat.2012.07.011

[11]   M. Ahmed, A. J. Byrne, J. McLaughlin, A. Elhissi, D. A. Phoenix and W. Ahmed, “Vibrational and AFM Studies of Adsorption of Glycine on DLC and Silicon-Doped DLC,” Journal of Materials Science, Vol. 47, No. 4, 2012, pp. 1729-1736. doi:10.1007/s10853-011-5952-3

[12]   C. Liu, G. Q. Li, W. X. Chen, Z. X. Mu, C. W. Zhang and L. Wang, “The Study of Doped DLC Films by Ti Ion Implantation,” Thin Solid Films, Vol. 475, No. 1-2, 2005, pp. 279-282. doi:10.1016/j.tsf.2004.08.051

[13]   G. H. Hsiue, S. D. Lee, P. C. T. Chang and C. Y. Kao, “Surface Characterization and Biological Properties Study of Silicone Rubber Membrane Grafted with Phospholipid as Biomaterial via Plasma Induced Graft Copolymerization,” Journal of Biomedical Materials Research, Vol. 42, No. 1, 1998, pp. 134-147. doi:10.1002/(SICI)1097-4636(199810)42:1<134::AID-JBM17>3.0.CO;2-L

[14]   J. C. Damasceno, S. S. Camargo Jr., F. L. Freire Jr. and R. Carius, “Deposition of Si-DLC Films with High Hardness, Low Stress and High Deposition Rates,” Surface and Coatings Technology, Vol. 133-134, 2000, pp. 247-252. doi:10.1016/S0257-8972(00)00932-4

[15]   N. Moolsradoo, S. Abe and S. Watanabe, “Thermal Stbility and Tribological Performance of DLC-SiO Films,” Advances in Materials Science and Engineering, Vol. 2011, 2011, Article ID: 483437.

[16]   S. Kelly, E. M. Regan, J. B. Uney, A. D. Dick, J. P. McGeehan, E. J. Mayer and F. Claeyssens, “Patterned Growth of Neuronal Cells on Modified Diamond-Like Carbon Substrates,” Biomaterials, Vol. 29, No. 24-25, 2008, pp. 2573-2580. doi:10.1016/j.biomaterials.2008.03.001

[17]   M. Ahmed, J. Anthony Byrne and J. A. D. McLaughlin, “Glycine Adsorption onto DLC and N-DLC Thin Films Studied by XPS and AFM,” e-Journal of Surface Science and Nanotechnology, Vol. 7, 2009, pp. 217-224. doi:10.1380/ejssnt.2009.217

[18]   A. Bendavid, P. J. Martin, C. Comte, E. W. Preston, A. J. Haq, F. S. M. Ismail and R. K. Singh, “The Mechanical And Biocompatibility Properties of DLC-Si Films Prepared by Pulsed DC Plasma Activated Chemical Vapor Deposition,” Diamond & Related Materials, Vol. 16, No. 8, 2007, pp. 1616-1622. doi:10.1016/j.diamond.2007.02.006

[19]   Q. Zhao, Y. Liu, C. Wang and S. Wang, “Bacterial Adhesion on Silicon-Doped Diamond-Like Carbon Films,” Diamond and Related Materials, Vol. 16, No. 8, 2007, pp. 1682-1687. doi:10.1016/j.diamond.2007.03.002

[20]   R. K. Roy, S. J. Park, H. W. Choi1, K. R. Lee, J. H. Kim, D. K. Han, J. H. Shin, H. G. Kim, S. H. Ahn, J. G. Kim, S. J. Park and K. R. Lee, “Hemocompatibility of Surface Modified Si Incorporated Diamond-Like Carbon Films,” Thin Solid Films, Vol. 482, 2005, pp. 299-304.

[21]   R. Paul, S. N. Das, S. Dalui, R. N. Gayen, R. K. Roy, R. Bhar and A. K. Pal, “Synthesis of DLC Films with Different sp2/sp3 Ratios and Their Hydrophobic Behaviour,” Journal of Physics D: Applied Physics, Vol. 41, No. 5, 2008, Article ID: 055309. doi:10.1088/0022-3727/41/5/055309

[22]   W. J. Hsieh, P. S. Shih, C. C. Lin, C. Y. Wang and H. C. Shi, “Structure and Optical Property of the a-C:N Films Synthesized by Filtered Cathode Vacuum Arc,” Surface and Coatings Technology, Vol. 200, No. 10, 2006, pp. 3175-3178. doi:10.1016/j.surfcoat.2005.07.038

[23]   M. N. Huda, Y. Yan and M. M. Al-Jassim, “On the Existence of Si-C Double Bonded Graphene-Like Layers,” Chemical Physics Letters, Vol. 479, No. 4-6, 2009, pp. 255-258. doi:10.1016/j.cplett.2009.08.028

[24]   N. Fainer, Y. Rumyantsev, M. Kosinova, E. Maximovski, V. Kesler, V. Kirienko and F. Kuznetsov, “Low-k Dielectrics on Base of Silicon Carbon Nitride Films,” Surface and Coatings Technology, Vol. 201, No. 22, 2007, pp. 9269-9274. doi:10.1016/j.surfcoat.2007.04.046

[25]   H. Yoneyama and T. Torimoto, “Titanium Dioxide/Adsorbent Hybrid Photocatalysts for Photodestruction of Organic Substances of Dilute Concentrations,” Catalysis Today, Vol. 58, No. 2-3, 2000, pp. 133-140.

[26]   T. Kesvatera, B. Jonsson, A. Telling, V. Tougu, H. V. E. Thulin and S. Linse, “A Protein Optimized for Calcium Binding at Neutral pH,” Biochemistry, Vol. 40, No. 50, 2001, pp. 15334-15340.

[27]   H. J. Kim, I.-S. Bae, S.-J. Cho, J.-H. Boo, B.-C. Lee, J. Heo, I. Chung and B. Hong, “Synthesis and Characteristics of NH2-Functionalized Polymer Films to Align and Immobilize DNA Molecules N-Containing Moieties Could Be Useful,” Nanoscale Research Letters, Vol. 7, 2012, pp. 30-37. doi:10.1186/1556-276X-7-30

[28]   M. Bouchard, J. Zurdo, E. J. Nettleton, C. M. Dobson, and C. V. Robinson, “Formation of Insulin Amyloid Fibrils Followed by FTIR Simultaneously with CD and Electron Microscopy,” Protein Science, Vol. 9, No. 10, 2000, pp. 1960-1967. doi:10.1110/ps.9.10.1960

[29]   M. Jackson and H. H. Mantsch, “The Use and Misuse of FTIR Spectroscopy in the Determination of Protein Structure,” Critical Reviews in Biochemistry and Molecular Biology, Vol. 30, No. 2, 1995, pp. 95-120. doi:10.3109/10409239509085140

[30]   K. L. Munro, K. R. Bambery, E. A. Carter, L. Puskar, M. J. Tobin, B. R. Wood and C. T. Dillon, “Synchrotron Radiation Infrared Microspectroscopy of Arsenic-Induced Changes to Intracellular Biomolecules in Live Leukaemia Cells,” Vibrational Spectroscopy, Vol. 53, No. 1, 2010, pp. 39-44. doi:10.1016/j.vibspec.2010.02.004

[31]   C. Chadefaux, A.-S. L. Ho, L. Bellot-Gurlet and I. Reiche, “Curve-Fitting Micro-ATR-FTIR Studies of the Amide I and II Bands of Type I Collagen Archaeological Bone Materials,” e-Preservation Science, Vol. 6, 2009, pp. 129-137.

[32]   A. Bouhekka and T. Burgi, “In Situ ATR-IR Spectroscopy Study of Adsorbed Protein: Visible Light Denaturation of Bovine Serum Albumin on TiO2,” Applied Surface Science, Vol. 261, 2012, pp. 369-374. doi:10.1016/j.apsusc.2012.08.017

[33]   M. W. Khan, Z. Rasheed, W. A. Khan and R. Ali, “Biochemical, Biophysical, and Thermodynamic Analysis of in Vitro Glycated Human Serum Albumin,” Biochemistry (Moscow), Vol. 72, No. 2, 2007, pp. 146-152. doi:10.1134/S0006297907020034

[34]   M. L. Gulrajani, K. P. Brahma, P. S. Kumar and R. Purwar, “Application of Silk Sericin to Polyester Fabric,” Journal of Applied Polymer Science, Vol. 109, No. 1, 2008, pp. 314-321. doi:10.1002/app.28061

[35]   M. Dumitrascu, V. Meltzer, E. Sima, M. Virgolici, M. G. Albu, A. Ficai, V. Moise, R. Minea, C. Vancea, A. Scarisoreanu and F. Scarlat, “Characterization of Electron Beam Irradiated Collagen-Polyprolidone (PVP) and CollagenDexran (DEX) Blends,” Digest Journal of Nanomaterials and Biostructures, Vol. 6, 2011, pp. 1793-1803.

[36]   P. Juszczyk, A. S. Kolodziejczyk and Z. Grzonka, “FTIR Spectroscopic Studies on Aggregation Process of the βAmyloid 11-28 Fragment and Its Variants,” Journal of Peptide Science, Vol. 15, No. 1, 2009, pp. 23-29. doi:10.1002/psc.1085

 
 
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