Development of Dermal cell line has great scope in the field of skin related diseases and regenerative medicine. Alopecia leads to a skin disorder causing balding and its mechanism is not yet understood. In the present study, we have developed and characterized a heterogeneous population of human dermal mesenchymal-like stem cell line from scalp biopsy of androgenetic alopecia patient with a view to isolate cells from the bulge region of the hair follicle. Our study showed that the dermal cells isolated from dermis of skin showed epithelial-like cells expressing CD34 and Keratin 18, which are characteristic of bulge hair follicle cells. These cells also expressed mesenchymal phenotypes and pluripotency markers such as Oct4, Nanog and SOX2. These cells were designated as “Human Dermal Mesenchymal-like Stem Cells (hDMSCs)”. To confirm their epithelial phenotypes, we have grown these cells at low serum concentration and it was observed that 3% serum concentration provided optimum conditions for their growth and maintenance of characteristics. The hDMSCs cells are presently at passage 10. This study reports the establishment of human dermal mesenchymal-like cell line from the dermis of Alopecia patient, which may be used as an in vitro model system to study the mechanism of Alopecia and other related skin disorders.
 Potdar, P.D. and Sutar, J.P. (2010) Establishment and molecular characterization of mesenchymal stem cells derived from human visceral and subcutaneous adipose tissue. Journal of Stem cells and Regenerative Medicine, 6, 26-45.
 Potdar, P.D. and Chaugule, S. (2011) Establishment and molecular characterization of breast cancer mesenchymal stem cell line derived from human non-metastasis breast cancer tumor. Stem Cell Discovery, 1, 21-28. doi:10.4236/scd.2011.12003
 Lau, K., Paus, R., Tiede, S., Day, P. and Bayat, A. (2011). Exploring the role of stem cells in cutaneous wound healing. Experimental Dermatology, 18, 921-933. doi:10.1111/j.1600-0625.2009.00942.x
 Shin, S.H., Park, S.Y., Kim, M.K., Kim, J.C. and Sung, Y.K. (2011) Establishment and characterization of an immortalized human dermal papilla cell line. Biochemistry and Molecular Biology Reports, 44, 512-516.
 Trempus, C.S., Morris, R.J., Bortner, C.D., Cotsarelis, G., Faircloth, R.S., Reece, J.M. and Tennant, R. (2002). Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34. Journal of Investigative Dermatology, 120, 501-511. doi:10.1046/j.1523-1747.2003.12088.x
 Garza, L.A., Yang, C., Zhao, T., Blatt, H.B., Lee, M., He, H., Stanton, D.C., Carrasco, L., Spiegel, J.H., Tobias, J.W. and Cotsarelis, G. (2011) Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells. The Journal of Clinical Investigation, 121, 613-622. doi:10.1172/JCI44478
 Vaculik, C., Schuster, C., Bauer, W., Iram, N., Pfisterer, K., Kramer, G., Reinisch, A., Strunk, D. and Elbe-Bürger, A. (2011) Human dermis harbors distinct mesenchymal stromal cell subsets. Journal of Investigative Dermatology, 132, 563-574. doi:10.1038/jid.2011.355
 Ilio, K.Y., Sensibar, J.A. and Lee, C. (1995) TGF-beta1, TGF-alpha and EGF on cell proliferation and cell death in rat ventral prostatic epithelial cells in culture. Journal of Andrology, 16, 482-490.
 Brink, H.E., Stalling, S.S. and Nicoll S.B. (2005). Influence of serum on adult and fetal sermal fibroblast migration, adhesion, and collagen expression. In Vitro Cellular & Developmental Biology—Animal, 41, 252-257. doi:10.1290/0503023R.1
 Potdar, P.D. and Subedi, R.P. (2011) Defining molecular phenotypes of mesenchymal and hematopoietic stem cells derived from peripheral blood of acute lymphocytic leukemia patients for regenerative stem cell therapy. Journal of Stem cells and Regenerative Medicine, 7, 29-40.
 Kirnbauer, R., Kock, A., Schwarz, T., Urbanski, A., Krutmann, J., Borth, W., Damm, D., Shipley, G., Ansel, J.C. and Luger, T.A. (1989). IFN-beta 2, B cell differentiation factor 2, or hybridoma growth factor (IL-6) is expressed and released by human epidermal cells and epidermoid carcinoma cell lines. The Journal of Immunology, 142, 1922-1928.