AJPS  Vol.4 No.4 , April 2013
Flash microbiocide: A Rapid and Economic Method for Determination of MBC and MFC
ABSTRACT

Although nowadays there are methods for determining the Minimal Bactericidal Concentration and Minimal Fungicidal Concentration, it is indispensable that the establishment of innovative methodologies could be more practical and cheaper. The new methodology Flash microbiocide is an assay in which one aliquot from 96 well plate of Minimal Inhibitory Concentration test is transferred to another plate containing different culture medium. The correspondence with the reference methods described in the National Committee for Clinical Laboratory Standards (NCCLS-CLSI) document M26-A was achieved, denoting the efficiency of this fast and simple method.


Cite this paper
C. Hernandes, J. Coppede, B. Bertoni, S. França and A. Pereira, "Flash microbiocide: A Rapid and Economic Method for Determination of MBC and MFC," American Journal of Plant Sciences, Vol. 4 No. 4, 2013, pp. 850-852. doi: 10.4236/ajps.2013.44104.
References
[1]   W. M. Scheld and M. A. Sande, “Bactericidal versus Bacteriostatic Antibiotic Therapy of Experimental Pneumococcal Meningitis in Rabbits,” Journal of Clinical Investigation, Vol. 71, No. 3, 1983, pp. 411-419. doi:10.1172/JCI110785

[2]   J. P. Sculier and J. Klastersky, “Significance of Serum Bactericidal Activity in Gram-Negative Bacillary Bacteremia in Patients with and without Granulocytopenia,” American Journal of Medicine, Vol. 76, No. 3, 1984, pp. 429-435. doi:10.1016/0002-9343(84)90662-4

[3]   Clinical and Laboratory Institute, “Methods for Determining Bactericidal Activity of Antimicrobial Agents,” Approved Guideline, CLSI Document M26-A, Wayne, 1999.

[4]   S. Handwerger and A. Tomaz, “Antibiotic Tolerance among Clinical Isolates of Bacteria,” Reviews of Infectious Diseases, Vol. 7, No. 3, 1985, pp. 368-386. doi:10.1093/clinids/7.3.368

[5]   G. A. Pankey and L. D. Sabath, “Clinical Relevance of Bacteriostatic versus Bactericidal Mechanisms of Action in the Treatment of Gram Positive Bacterial Infections,” Clinical Infectious Diseases, Vol. 38, No. 6, 2004, pp. 864-870. doi:10.1086/381972

[6]   Clinical and Laboratory Institute, “Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically,” 6th Edition, CLSI Document M7-A6, Wayne, 2003.

[7]   Clinical and Laboratory Institute, “Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts,” 3rd Edition, CLSI Document M27-A2, Wayne, 2002.

[8]   Clinical and Laboratory Institute, “Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi,” 2nd Edition, CLSI Document M38-A, Wayne, 2008.

[9]   A. L. Barry and R. A. Lasner, “In Vitro Methods for Determining Minimum Lethal Concentrations of Antibacterial Agents,” American Journal of Clinical Pathology, Vol. 71, No. 1, 1979, pp. 88-92.

[10]   J. Dankert, Y. Holloway, W. Joldersma and J. Hess, “Important of Minimizing Carry over Effect at Subculture in the Detection of Penicillin Tolerant Viridans Group Streptococci,” Antimicrobial Agents and Chemotherapy, Vol. 23, No. 4, 1983, pp. 232-235. doi:10.1128/AAC.23.4.614

 
 
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