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 AJPS  Vol.4 No.4 , April 2013
Effect of Jasmonic Acid on Photosynthetic Pigments and Stress Markers in Cajanus cajan (L.) Millsp. Seedlings under Copper Stress
Abstract: Jasmonates are class of plant growth regulators act as signal molecule that intercede various components in physiological and metabolic regulation, stress responses and possibly communication through signal transduction. Oxidative stress due to heavy metal exposure stimulates synthesis and activity of antioxidant metabolites and enhances antioxidant enzyme activities that could protect plant tissues. The aim of this study was to investigate the exogenous effect of JA at seed level which can transduce throughout seedling growth and regulate antioxidant activities such as superoxide dismutase (SOD; EC 1.15.1.1) and guaiacol peroxidase (POD; EC 1.11.1.7) in 12 days old seedlings of pigeon pea (Cajanus cajan (L.) Millsp.) in presence and/or absence of copper. The activity of SOD and POD increased significantly in presence of Cu2+ after seed priming with JA. JA also helps in chlorophyll and carotenoid accumulation and neutralizes the toxic effect of Cu2+ on seedlings. This is the first report of JA effect on photosynthetic pigment accumulation and H2O2 mitigating enzymes i.e. SOD and POD and it could be recommended that seed priming with JA help in ameliorating toxic effect of Cu2+.
Cite this paper: S. Poonam, H. Kaur and S. Geetika, "Effect of Jasmonic Acid on Photosynthetic Pigments and Stress Markers in Cajanus cajan (L.) Millsp. Seedlings under Copper Stress," American Journal of Plant Sciences, Vol. 4 No. 4, 2013, pp. 817-823. doi: 10.4236/ajps.2013.44100.
References

[1]   Y. He, H. Fukushige, D. F. Hildebrand and S. Gan, “Evidence Supporting a Role of Jasmonic Acid in Arabidopsis Leaf Senescence,” Plant Physiology, Vol. 128, No. 3, 2002, pp. 876-884. doi:10.1104/pp.010843

[2]   R. A. Weidhase, J. Lehmann, H. Kramell, G. Sembdner and B. Partier, “Degradation of Ribulose-1, 5-Biphosphate Carboxylase and Chlorophyll in Senescing Barley Leaf Segments Triggered by Jasmonic Acid Methylester, and Counteraction by Cytokinin,” Physiologia Plantarum, Vol. 69, No. 1, 1987, pp. 161-166. doi:10.1111/j.1399-3054.1987.tb01961.x

[3]   C. Wasternack and B. Hause, “Jasmonates and Octadecanoids: Signals in Plant Stress Responses and Development,” Progress in Nucleic Acid Research and Molecular Biology, Vol. 72, 2002, pp. 165-221. doi:10.1016/S0079-6603(02)72070-9

[4]   E. D. Ananiev, K. Ananieva, and I. Todorov, “Effect of Methyl Ester of Jasmonic Acid, Abscisic Acid and Benzyladenine on Chlorophyll Synthesis in Excised Cotyledons of Cucurbita pepo (Zucchini),” Bulgarian Journal of Plant Physiology, Vol. 30, 2004, pp. 51-63.

[5]   R. Dhankar and R. Solanki, “Effect of Copper and Zinc Toxicity on Physiological and Biochemical Parameters in Vigna mungo (L.) Hepper,” International Journal of Pharma and Bio Science, Vol. 2, No. 2, 2011, p. 553.

[6]   A. Nicholls and M. Tarun, “Effects of Lead and Copper Exposure on Growth of an Invasive Weed, Lythrum salicaria L. (Purple Loosestrife),” The Ohio Journal of Science, Vol. 103, No. 5, 2003, pp. 129-133.

[7]   V. Agrawal and K. Sharma, “Phytotoxic Effects of Cu, Zn, Cd and Pb on in Vitro Regeneration and Concomitant Protein Changes in Holarrhena antidysenterica,” Biologia Plantarum, Vol. 50, No. 2, 2006, pp. 307-310. doi:10.1007/s10535-006-0027-z

[8]   H. K. Lichtenthaler, “Chlorophylls and Carotenoids: Pigments of Photosynthetic Biomembranes,” In: L. Packer and R. Douce, Eds., Methods in Enzymology, Vol. 148, Academic Press, New York, 1987, pp. 350-382.

[9]   R. L. Heath and L. Packer, “Photoperoxidation in Isolated Chloroplast. 1. Kinetics and Stoichiometry of Fatty Acid Peroxidation,” Archives of Biochemistry and Biophysics, Vol. 125, No. 1, 1968, pp. 189-198. doi:10.1016/0003-9861(68)90654-1

[10]   L. S. Bates, R. P. Waldren and I. D. Teare, “Rapid Determination of Free Proline for Water Stress Studies,” Plant and Soil, Vol. 39, No. 1, 1973, pp. 205-208. doi:10.1007/BF00018060

[11]   H. Lowry, N. J. Rosebrough, A. L. Farr and R. J. Randall, “Protein Estimation with Folin-Phenol Reagent,” Journal of Biological Chemistry, Vol. 193, 1951, p. 265.

[12]   Y. Kono, “Generation of Superoxide Radical during Autoxidation of Hydroxylamine and an Assay for Superoxide Dismutase,” Archives of Biochemistry and Biophysics, Vol. 186, No. 1, 1978, pp. 189-195. doi:10.1016/0003-9861(78)90479-4

[13]   J. Putter, “Peroxidase,” In: H. U. Bergmeyer, Ed., Methods of Enzymatic Analysis, Verlag Chemie, Weinhan, 1974, pp. 685-690.

[14]   M. E. Sorial and A. A. Gendy, “Response of Sweet Basil to Jasmonic Acid Application in Relation to Different Water Supplies,” Bioscience Research, Vol. 7, No. 1, 2010, pp. 39-47.

[15]   S. I. Beale, “δ-Aminolevulinic Acid in Plants: Its Biosynthesis, Regulation and Role in Plastid Development,” Annual Review of Plant Physiology, Vol. 29, 1978, pp. 95-120. doi:10.1146/annurev.pp.29.060178.000523

[16]   M. Kovac and M. Ravnikar, “The Effect of Jasmonic Acid on the Photosynthetic Pigments of Potato Plant Grown in Vitro,” Plant Sciences, Vol. 103, No. 1, 1994, pp. 11-17. doi:10.1016/0168-9452(94)03974-7

[17]   R. A. Fletcher and D. McCullagh, “Benzyladenine as a Regulator of Chlorophyll Synthesis in Cucumber Cotyledons,” Canadian Journal of Botany, Vol. 49, No. 12, 1971, pp. 2197-2201. doi:10.1139/b71-307

[18]   R. Lew and H. Tsuji, “Effect of Benzyladenine Treatment Duration on D-Aminolevulinic Acid Accumulation in the Dark, Chlorophyll Lag Phase Abolition, and Long Term Chlorophyll Production in Excised Cotyledons of Dark Grown Seedlings,” Plant Physiology, Vol. 69, No. 3, 1982, pp. 663-667. doi:10.1104/pp.69.3.663

[19]   C. Reiss and S. I. Beale, “External Calcium Requirements for Light Induction of Chlorophyll Accumulation and Its Enhancement by Red Light and Cytokinin Pre-Treatment in Excised Etiolated Cucumber Cotyledons,” Planta, Vol. 196, No. 4, 1995, pp. 635-641. doi:10.1007/BF01106754

[20]   M. Orozco-Cardenas and C. A. Ryan, “Hydrogen Peroxide Is Generated Systemically in Plant Leaves by Wounding and Systemin via the Octadecanoid Pathway,” Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 11, 1999, pp. 6553-6557. doi:10.1073/pnas.96.11.6553

[21]   G. J. Kumari, A. M. Reddy, S. T. Naik, S. G. Kumar, J. Prasanthi, G. Sriranganayakulu and P. C. Reddy, “Jasmonic Acid Induced Changes in Protein Pattern, Antioxidative Enzyme Activities and Peroxidase Isozymes in Peanut Seedlings,” Biologia Plantarum, Vol. 50, No. 2, 2006, pp. 219-226. doi:10.1007/s10535-006-0010-8

[22]   I. S. Fedina and K. M. Benderliev, “Response of Scenedesmus incrassatulus to Salt Stress as Affected by Methyl Jasmonate,” Biology of Plant, Vol. 43, No. 4, 2000, pp. 625-627. doi:10.1023/A:1002816502941

[23]   H. Bandurska, A. Stroinski and K. Jan, “The Effect of Jasmonate on the Accumulation of ABA, Proline and Its Influence on Membrane Injury under Water Deficient in Two Barley Genotypes,” Acta Physiologeiae Plantarum, Vol. 25, No. 3, 2003, pp. 279-285. doi:10.1007/s11738-003-0009-0

[24]   C. Chen and C. Kao, “Osmotic Stress and Water Stress Have Opposite Effects on Putrascine and Proline Production in Excised Rice Leaves,” Plant Growth Regulation, Vol. 13, No. 2, 1993, pp. 197-202. doi:10.1007/BF00024262

[25]   X. Gao, X. Wang, Y. Lu, L. Zhang, Y. Shen, Z. Liang and D. Zhang, “Jasmonic Acid Is Involved in the Water Stress Induced Betaine Accumulation in Pear Leaves,” Plant Cell and Environment, Vol. 27, No. 4, 2004, pp. 5497-5507. doi:10.1111/j.1365-3040.2004.01167.x

[26]   D. Walters, T. Colwey and A. Mitchell, “Methyl Jasmonate Alters Polyamine Metabolism and Induced Systemic Protection against Powdery Infection in Barley Seedlings,” Journal of Experimental Botany, Vol. 53, No. 269, 2002, pp. 747-756. doi:10.1093/jexbot/53.369.747

[27]   H. S. Seo, J. T. Song, J. J. Cheong, Y. H. Lee, Y. W. Lee, I. Hwang and J. S. Lee, “Jasmonic Acid Carboxyl Methyltransferase: A Key Enzyme for Jasmonate-Regulated Plant Responses,” Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 8, 2001, pp. 4788-4793. doi:10.1073/pnas.081557298

[28]   S. Reinbothe, C. Reinbothe, C. Heintzen, C. Seidenbecher, and B. Parthier, “A Methyl Jasmonate-Induced Shift in the Length of the 5’ Untranslated Region Impairs Translation of the Plastid Rbcl Transcript in Barley,” The EMBO Journal, Vol. 12, No. 4, 1993, pp. 1505-1512.

[29]   M. A. Matamoros, D. A. Dalton, J. Ramos, M. R. Clemente, M. C. Rubio and M. Becana, “Biochemistry and Molecular Biology of Antioxidants in the Rhizobia- Legume Symbiosis,” Plant Physiology, Vol. 133, No. 2, 2003, pp. 499-509. doi:10.1104/pp.103.025619

[30]   R. Rakwal and S. Komatsu, “Jasmonic Acid-Induced Necrosis and Drastic Decreases in Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase in Rice Seedlings under Light Involves Reactive Oxygen Species,” Journal of Plant Physiology, Vol. 158, No. 6, 2001, pp. 679-688. doi:10.1078/0176-1617-00372

 
 
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