WJA  Vol.3 No.1 , March 2013
Intracellular Transport of HIV-1 Matrix Protein Associated with Viral RNA
Abstract: HIV-1 matrix protein (MA) is a multifunctional structural protein localized on N terminus of Gag precursor p55. MA participates in HIV-1 assembly as membranotropic part of Gag precursor as well as an individual protein spliced from Gag early in infection. MA is found in the nuclei of infected cells and in plasma membrane, the site of virus assembly, in association with viral genome RNA. MA mutated variant M4 which contains two changed amino acids in N-terminal regions is also associated with viral RNA, but it is localized in the nuclear and cytoskeleton fractions but not in the plasma membrane suggesting that the mutant is deprived of membranotropic signal and “sticks” in the nuclei an d cytoskeleton, its previous location sites. These data allow suggesting that MA involved into transmission of viral RNA is transported to plasma membrane by cytoskeleton.
Cite this paper: A. Gozhenko, V. Divocha, G. Vorkunova, A. Bukrinskaya and S. Lupandin, "Intracellular Transport of HIV-1 Matrix Protein Associated with Viral RNA," World Journal of AIDS, Vol. 3 No. 1, 2013, pp. 33-35. doi: 10.4236/wja.2013.31005.

[1]   A. G. Bukrinskaya, G. K. Vorkunova and Y. Y. Tentsov, “HIV-1 Matrix Protein P17 Resides in Cell Nuclei in Association with Genomic RNA,” AIDS Research and Human Retroviruses, Vol. 8, No. 10, 1992, pp. 1795-1801. doi:10.1089/aid.1992.8.1795

[2]   S. Dupont, N. Sharova, C. DeHoratius, C.-M. A. Virbasius, X. C. Zhu, A. G. Bukrinskaya, M. Stevenson and M. R. Green, “A Novel Nuclear Export Activity in HIV-1 Matrix Protein Required for Viral Replication,” Nature, Vol. 402, No. 6762, 1999, pp. 681-685. doi:10.1038/45272

[3]   A. H. Kaplan and R. Swanstrom, “HIV-1 Gag Proteins Are Processed in Two Cellular Compartments,” Proceedings of the National Academy of Sciences of USA, Vol. 88, No. 10, 1991, pp. 4528-4532. doi:10.1073/pnas.88.10.4528

[4]   A. Bukrinskaya, “HIV-1 Matrix Protein: A Mysterious Regulator of the Viral Life Cycle,” Virus Research, Vol. 124, No. 1-2, 2007, pp. 1-11. doi:10.1016/j.virusres.2006.07.001

[5]   N. Sharova and A. Bukrinskaya, “P17 and P17-Containing Gag Precursor of Input HIV Are Transported into the Nuclei of Infected Cells,” AIDS Research and Human Retroviruses, Vol. 7, No. 3, 1991, pp. 303-306. doi:10.1089/aid.1991.7.303

[6]   T. M. J. Niederman, W. R. Hastings and L. Ratner, “Myristoylation-Enhanced Binding of the HIV-1 Net Protein to T Cell Skeletal Matrix,” Virology, Vol. 197, No. 1, 1993, pp. 420-425. doi:10.1006/viro. 1993.1605

[7]   A. Bukrinskaya, B. Brichacek, A. Mann and M. Stevenson, “Establishment of a Functional HIV-1 Reverse Transcription Complex Involves the Cytoskeleton,” The Journal of Experimental Medicine, Vol. 188, No. 11, 1998, pp. 2113-2125. doi:10.1084/jem.188.11.2113