Although Coxiella burnetii is considered to be an obligate intracellular
bacterium and grows in embryonated eggs, laboratory animals and cell culture,
recently it has been grown in cell-free media and on agar plates. This current
study was conducted to compare four cell lines for their yield of C.burnetii. Four different isolates of C.burnetii (Henzerling, Arandale, Cumberland and Timony) were grown in DH82, L929, Vero
and XTC-2 cell lines.The DH82 and
XTC-2 cells lines produced the highest C.burnetii yield which was slightly less than
the yields achieved in recently published studies using cell free media. The
Arandale isolate of C. burnetii produced
a significantly higher yield in DH82 cells compared to XTC-2 cells (P<0.03).
Cite this paper
M. Lockhart, A. Islam, S. Fenwick, S. Graves and J. Stenos, "Growth Yields of Four Coxiella burnetii Isolates in Four Different Cell Culture Lines," Advances in Microbiology, Vol. 3 No. 1, 2013, pp. 88-90. doi: 10.4236/aim.2013.31014.
 W. Rumin, D. Kruszewska, W. Sadowski and S. Tylewska-Wierzbanowska, “Growth of Coxiella burnetii in Selected Cell Cultures,” Medycyna Doswiadczalna i Mikrobiologia, Vol. 42, No. 1-2, 1990, pp. 44-49.
 A. Omsland, et al., “Host Cell-Free Growth of the Q Fever Bacterium Coxiella burnetii,” Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 11, 2009, pp. 4430-4434.
 M. J. Roman, P. D. Coriz and O. G. Baca, “A Proposed Model to Explain Persistent Infection of Host Cells with Coxiella burnetii,” Journal of General Microbiology, Vol. 132, No. 5, 1986, pp. 1415-1422.
 T. F. McCaul, T. Hackstadt and J. C. Williams, “Ultrastructural and Biological Aspects of Coxliella burnetii under Physical Disruptions,” In: W. Burgdorfer and R. L. Anacker, Eds., Rickettsiae and Rickettsial Diseases, Academic Press, New York, 1981, pp. 267-280.
 O. G. Baca, et al., “Cell Cycle Distribution Patterns and Generation Times of L929 Fibroblast Cells Persistantly Infected with Coxiella burnetii,” Infection and Immunity, Vol. 47, No. 2, 1985, pp. 366-369.
 P. R. Burton, et al., “Some Ultrastructural Effects of Persistant Infections by the Rickettsia Coxiella burnetii in Mouse L Cells and Green Monkey Kidney (Vero) Cells,” Infection and Immunity, Vol. 21, No. 2, 1978, pp. 556-566.
 A. Omsland, et al., “Isolation From Animal Tissue and Genetic Transformation of Coxiella burnetii Are Facilitated by an Improved Axenic Growth Medium,” Applied and Environmental Microbiology, Vol. 77, No. 11, 2011, pp. 3720-3725. doi:10.1128/AEM.02826-10
 M. G. Lockhart, et al., “A Comparison of Methods for Extracting DNA from Coxiella burnetii as Measured by a Duplex qPCR Assay,” Letters in Applied Microbiology, Vol. 52, No. 5, 2011, pp. 514-520.
 L. R. Hendrix, J. E. Samuel and L. P. Mallavia, “Differentiation of Coxiella burnetii Isolates by Analysis of Restriction Endonuclease—Digested DNA Separated by SDS-PAGE,” Journal of General Microbiology, Vol. 137, No. 2, 1991, pp. 269-276.
 A. M. Denison, H. A. Thompson and R. F. Massung, “IS1111a Insertion Sequences of Coxiella burnetii: Charactarisation and Use For Repetitive Element PCR-Based Differentiation of Coxiella burnetii Isolates,” BMC Microbiology, Vol. 7, No. 91, 2007, pp. 91-98.
 T. Hackstadt and J. C. Williams, “Biochemical Stratagem for Obligate Parasitism of Eukaryotic Cells by Coxiella burnetii,” Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, No. 5, 1981, pp. 3240-3244. doi:10.1073/pnas.78.5.3240
 L. Hendrix and L. P. Mallavia, “Active Transport of Proline by Coxiella burnetii,” Journal of General Microbiology, Vol. 130, No. 11, 1984, pp. 2857-2863.
 T. F. McCaul and J. C. Williams, “Developmental Cycle of Coxiella burnetii: Structure and Morphogenesis of Vegetative and Sporogenic Differentiations,” Journal of Bacteriology, Vol. 147, No. 3, 1981, pp. 1063-1076.