AiM  Vol.3 No.1 , March 2013
Efficacy of Antimicrobial Lauric Arginate against Listeria monocytogenes on Stainless Steel Coupons

Commercially processed foods become contaminated with Listeria monocytogenes in post-processing environments where favorable conditions help the bacteria thrive. The US Food and Drug Administration has approved Lauric arginate (LAE) as generally recognized as safe (GRAS) for certain food applications. This study evaluated the efficacy of Mirenat-N (LAE dissolved in food-grade propylene glycol) against L. monocytogenes on food contact surfaces. A three-strain cocktail of L. monocytogenes was used to inoculate 24 polished stainless steel coupons with three treatments, 100 ppm and 200 ppm solutions of LAE and water (control); two sub-treatments of high (6 log CFU/ml) and low (4 log CFU/ml) inoculum levels; and two contact times of 5 and 15 min. Attached bacteria were dislodged by vortexing coupons for 1 min with 20 g of 3-mm solid glass beads in 10 ml of 0.1% peptone diluent, and bacterial populations were calculated by plating onto modified oxford medium (MOX) and thin agar layer MOX (TALMOX). The 100 ppm treatment showed average reductions of 1.38 and 2.57 log CFU/coupon at the low inoculum level and 0.37 and 0.62 log CFU/coupon at high inoculum levels, after 5 and 15 min exposure, respectively. For 200 ppm at the high inoculum level, 1.23 and 1.88 log CFU/coupon reductions were seen for 5 and 15 min, respectively; the low inoculum level at 5 and 15 min exposure showed reductions of ≤1.5 log CFU/coupon. The 100 ppm LAE treatment was more effective at low inoculum levels for 5 and 15 min contact times and may be used to control low levels of contamination of L. monocytogenes on food contact surfaces.

Cite this paper
J. Saini, M. Barrios, J. Marsden, K. Getty and D. Fung, "Efficacy of Antimicrobial Lauric Arginate against Listeria monocytogenes on Stainless Steel Coupons," Advances in Microbiology, Vol. 3 No. 1, 2013, pp. 65-68. doi: 10.4236/aim.2013.31010.
[1]   P. J. Edington, H. Gibso, J. T. Holah, P. S. Handley and P. Gilbert, “The Influence of Substratum Properties on Attachment of Bacterial Cells,” Colloids and Surfaces B, Vol. 5, No. 3-4, 1995, pp. 153-159. doi:10.1016/0927-7765(95)01219-9

[2]   J. W. Austin and G. Bergeron, “Development of Bacterial Biofilms in Dairy Processing Lines,” Journal of Dairy Research, Vol. 62, No. 3, 1995, pp. 509-549. doi:10.1017/S0022029900031204

[3]   D. G. Dunsmore, A. Twomey, W. G. Whittlestone and H. W. Morgan, “Design and Performance of Systems for Cleaning Product-Contact Surfaces of Food Equipment— A Review,” Journal of Food Protection, Vol. 44, No. 3, 1981, pp. 220-240.

[4]   R. E. Faust and D. A. Gabis, “Controlling Microbial Growth in Food Processing Environments,” Food Technology, Vol. 42, No. 12, 1988, pp. 81-82.

[5]   J. A. Lopes, “Evaluation of Dairy and Food Plant Sanitizers against Salmonella Typhimurium and Listeria monocytogenes,” Journal of Dairy Science, Vol. 69, No. 11, 1986, pp. 2791-2796. doi:10.3168/jds.S0022-0302(86)80731-7

[6]   R. Orth and H. Mrozek, “Is the Control of Listeria, Campylobacter, and Yersinia a Disinfection Problem?” Fleischwirtschaft, Vol. 69, No. 10, 1989, pp. 1575-1576.

[7]   Code of Federal Regulations (CFR), “Sanitizing Solutions,” 2011.

[8]   G. Bakal and A. Diaz, “The Lowdown on Lauric Arginate: Food Antimicrobial Hammers Away at Plasma Membrane, Disrupting a Pathogen’s Metabolic Process,” Food Quality, Vol. 12, No. 1, 2005, pp. 54-61.

[9]   D. Benford, R. Harrison, J. Larsen and M. DiNovi, “Safety Evaluation of Certain Food Additives: Ethyl Lauroyl Arginate,” World Health Organization, Geneva, 2009.

[10]   E. Rodríguez, J. Seguer, X. Rocabayera and A. Manresa, “Cellular Effects of Monohydrochloride of L-Arginine, N-Lauroyl Ethylester (LAE) on Exposure to Salmonella Typhimurium and Staphylococcus aureus,” Journal of Applied Microbiology, Vol. 96, No. 5, 2004, pp. 903-912. doi:10.1111/j.1365-2672.2004.02207.x

[11]   A. C. S. Porto-Fett, S. G. Campano, J. L. Smith, A. Oser, B. Shoyer, J. E. Call and J. B. Luchansky, “Control of Listeria monocytogenes on Commercially-Produced Frankfurters Prepared with and without Potassium Lactate and Sodium Diacetate and Surface Treated with Lauric Arginate Using the Sprayed Lethality in Container (SLIC) Delivery Method,” Meat Science, Vol. 85, No. 2, 2010, pp. 312-318. doi:10.1016/j.meatsci.2010.01.020

[12]   P. J. Toarmina and W. J. Dorsa, “Short-Term Bactericidal Efficacy of Lauric Arginate against Listeria monocytogenes Present on the Surface of Frankfurters,” Journal of Food Protection, Vol. 72, No. 6, 2009, pp. 1216-1224.

[13]   E. M. Martin, C. L. Griffis, K. L. S. Vaughn, C. A. O’Bryan, E. C. Friedly, J. A. Marcy, S. C. Ricke, P. G. Crandall and R. Y. Lary, “Control of Listeria monocytogenes by Lauric Arginate on Frankfurters Formulated with or without Lactate/Diacetate,” Journal of Food Science, Vol. 74, No. 6, 2009, pp. 237-241. doi:10.1111/j.1750-3841.2009.01196.x

[14]   D. H. Kang and D. Y. C. Fung, “Thin Agar Layer Method for Recovery of Heat-Injured Listeria monocytogenes,” Journal of Food Protection, Vol. 62, No. 11, 2009, pp. 1346-1349.

[15]   Food Safety and Inspection Service (FSIS), “Updated Compliance Guidelines to Control LM in Post-Lethality Exposed Ready-To-Eat Meat and Poultry Products,” 2006.

[16]   A. Parker, “Effective Cleaning and Sanitizing Procedures. JIFSAN Good Aquacultural Practices Program,” 2007.