AiM  Vol.3 No.1 , March 2013
Activation of Ethanol Production by Combination of Recombinant Ralstonia eutropha and Electrochemical Reducing Power
ABSTRACT

Ralstonia eutropha was genetically modified to induce ethanol production from glucose. An electrochemical bioreactor was prepared to generate electrochemical reducing power coupled to regeneration of NADH. Growing cells of recombinant R. eutropha produced about 29 mM of ethanol in conventional conditions and 56 mM of ethanol in electrochemically reduced conditions from 100 mM glucose. Grown cells of the recombinant produced about 52 mM of ethanol in conventional conditions and 142 mM of ethanol in electrochemically reduced condition from 100 mM glucose. These results are a clue that electrochemical reducing power can induce the recombinant R. eutropha to produce more ethanol coupled to increase of NADH/NAD+ ratio.


Cite this paper
B. Jeon, J. Yi, I. Jung and D. Park, "Activation of Ethanol Production by Combination of Recombinant Ralstonia eutropha and Electrochemical Reducing Power," Advances in Microbiology, Vol. 3 No. 1, 2013, pp. 42-45. doi: 10.4236/aim.2013.31006.
References
[1]   B. Y. Jeon, I. L. Jung and D. H. Park, “Enrichment and Isolation of CO2-Fixing Bacteria with Electrochemical Reducing Power as a Sole Energy Source,” Journal of Environmental Protection, Vol. 3, No. 1, 2012, pp. 55-60.

[2]   B. Y. Jeon, S. Y. Kim, Y. K. Park and D. H. Park, “Enrichment of Hydrogenotrophic Methanogens in Coupling with Methane Production Using Electrochemical Bioreactor,” Journal of Microbiology and Biotechnology, Vol. 19, No. 12, 2009, pp. 485-493.

[3]   D. H. Park and J. G. Zeikus. “Utilization of Electrically Reduced Neutral Red by Actinobacillus succinogenes: Physiological Function of Neutral Red in Membrane-Driven Fumarate Reduction and Energy Conservation,” Journal of Bacteriology, Vol. 181, No. 8, 1999, pp. 2403-2410.

[4]   D. H. Park, M. Laivenieks, M. V. Guettler, M. K. Jain and J. G. Zeikus, “Microbial Utilization of Electrically Reduced Neutral Red as the Sole Electron Donor for Growth and Metabolite Production,” Applied Environmental Microbiology, Vol. 65, No. 7, 1999, pp. 2912-2917.

[5]   H. S. Kang, B. K. Na and D. H. Park, “Oxidation of Butane to Butanol Coupled to Electrochemical Redox Reaction of NAD+/NADH,” Biotechnogical Letters, Vol. 29, No. 8, 2007, pp. 1277-1280. doi:10.1007/s10529-007-9385-7

[6]   B. Y. Jeon and D. H. Park, “Improvement of Ethanol Production by Electrochemical Redox Combination of Zymomonas mobilis and Saccharomyces cerevisiae,” Journal of Microbiology and Biotechnology, Vol. 20, No. 1, 2010, pp. 94-100.

[7]   W. J. Lee and D. H. Park, “Electrochemical Activation of Nitrate Reduction to Nitrogen by Ochrobactrum sp. G3-1 Using a Noncompartmented Electrochemical Bioreactor,” Journal of Microbiology and Biotechnology, Vol. 19, No. 8, 2009, pp. 836-844.

[8]   B. Bowien and B. Kusian, “Genetics and Control of CO2 Assimilation in the Chemoautotroph Ralstonia eutropha,” Archives of Microbiology, Vol. 17, No. 2, 2002, pp. 85-93. doi:10.1007/s00203-002-0441-3

[9]   S. Sichwart, S. Hetzler, D. Br?ker and A. Steinbüchel, “Extension of the Substrate Utilization Range of Ralstonia eutropha Strain H16 by Metabolic Engineering to Include Mannose and Glucose,” Applied Environmental Micryobiology, Vol. 77, No. 4, 2011, pp. 1325-1334. doi:10.1128/AEM.01977-10

[10]   R. Repaske, “Nutritional Requirement for Hydrogenomonas eutropha,” Journal of Bacteriology, Vol. 83, No. 2, 1962, pp. 418-422.

[11]   P. E. Stukus and B. T. Becicco, “Autotrophic and Heterotrophic Metabolism of Hydrogenomonas,” Journal of Bacteriology, Vol. 95, No. 4, 1968, pp. 1469-1475.

[12]   B. T. Decicco and P. E. Stukus, “Autotrophic and Heterotrophic Metabolism of Hydrogenomonas: Regulation of Autotrophic Growth by Organic Substrates,” Journal of Bacteriology, Vol. 101, No. 2, 1970, pp. 339-345.

[13]   B. Y. Jeon, I. L. Jung and D. H. Park, “Enrichment of CO2-Fixing Bacteria in Cylinder-Type Electrochemical Bioreactor with Built-In Anode Compartment,” Journal of Microbiology and Biotechnology, Vol. 21, No. 6, 2011, pp. 590-598.

[14]   L. O. Ingram and T. Conway, “Expression of Different Levels of Ethanologenic Enzymes from Zymomonas mobilis in Recombinant Strains of Escherichia coli,” Applied Environmental Microbiology, Vol. 54, No. 2, 1988, pp. 397-404.

[15]   H. J. Tsai, K. L. Lin, J. C. Kuo and S. W. Chen, “Highly Efficient Expression of Fish Growth Hormone by Escherichia coli Cells,” Applied Environmental Microbiology, Vol. 61, No. 11, 1995, pp. 4116-4119.

[16]   L. O. Ingram, T. Conway, D. P. Clark, G. W. Sewell and J. F. Preston, “Genetic Engineering of Ethanol Production in Escherichia coli,” Applied Environmental Microbiology, Vol. 53, No. 10, 1987, pp. 2420-2425.

[17]   S. T. Taghavi, D. Van der Lelie and M. Mergeay, “Electroporation of Alcaligenes eutropha with (Mega) Plasmids and Genomic DNA Fragments,” Applied Environmental Microbiology, Vol. 60, No. 10, 1994, pp. 3685-3591.

[18]   T. Conway, G. W. Sewell, Y. A. Osman and L. O. Ingram, “Cloning and Sequencing of the Alcohol Dehydrogenase П Gene from Zymomonas mobilis,” Journal of Bacteriology, Vol. 169, No. 6, 1987, pp. 2591-2597.

[19]   J. Swings and J. De Ley, “The Biology of Zymomonas,” Bacteriological Review, Vol. 41, No. 1, 1977, pp. 1-46.

[20]   J. E. McGhee, G. St. Julian and R. W. Detroy, “Continuous and Static Fermentation of Glucose to Ethanol by Immobilized Saccharomyces cerevisiae Cells of Different Ages,” Applied Environmental Micryobiology, Vol. 44, No. 1, 1982, pp. 19-22.

[21]   S. Bringer-Meyer and H. Sahm, “Metabolic Shifts in Zymomonas mobilis in Response to Growth Conditions,” FEMS Microbiological Review, Vol. 54, No. 2, 1988, pp. 131-142. doi:10.1111/j.1574-6968.1988.tb02739.x

[22]   S. Bringer, R. K. Finn and H. Sahm. “Effect of Oxygen on the Metabolism of Zymomonas mobilis,” Archives of Microbiology, Vol. 139, No. 4, 1984, pp. 376-381. doi:10.1007/BF00408383

[23]   D. H. Park and J. G. Zeikus, “Electricity Generation in Microbial Fuel Cells Using Neutral Red as an Electronophore,” Applied Environmental Microbiology, Vol. 66, No. 4, 2000, pp. 1292-1297. doi:10.1128/AEM.66.4.1292-1297.2000

 
 
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