OJVM  Vol.3 No.1 , March 2013
Comparison of Antibody Detection with Yeast Lysate Antigens Prepared from Blastomyces dermatitidis Dog Isolates from Wisconsin and Tennessee
Abstract: Blastomyces dermatitidis, the causative agent of blastomycosis, a potentially lethal dimorphic fungal disease of humans and animals has been difficult to diagnose in the clinical laboratory. We are attempting to develop and improve immunodiagnostic assays by producing novel yeast lysate reagents for the detection of antibodies in blastomycosis. The objective of this study was to use lysate antigens prepared from four B. dermatitidis antigens isolated from dogs infected with blastomycosis from two different endemic areas (Wisconsin and Tennessee) testing for the detection of antibodies in serum specimens from immunized rabbits and infected dogs using the indirect ELISA. In the dog sera, absorbance values ranged from 0.774 to 1.350, while the rabbit sera values ranged from 0.533 to 1.191. Antigen T-58 appeared to lack any geographical specificity in antibody detection, which could prove useful in future immunodiagnostic detection of blastomycosis infections.
Cite this paper: J. Roberts, M. Madrid, L. Dickerson, B. Hutchison and G. Scalarone, "Comparison of Antibody Detection with Yeast Lysate Antigens Prepared from Blastomyces dermatitidis Dog Isolates from Wisconsin and Tennessee," Open Journal of Veterinary Medicine, Vol. 3 No. 1, 2013, pp. 67-72. doi: 10.4236/ojvm.2013.31011.

[1]   A. F. DiSalvo, “Blastomycosis, in Topley and Wilson’s Microbiology and Microbial Infections,” 9th Edition, Arnold Publishers, London, 1998, pp. 337-355.

[2]   R. W. Bradsher, “Clinical Features of Blastomycosis,” Seminars in Respiratory Infections, Vol. 12, No. 3, 1997, pp. 229-234.

[3]   R. W. Bradsher, S. W. Chapman and P. G. Pappas, “Blastomycosis,” Infectious Disease Clinics of North America, Vol. 17, No. 1, 2003, pp. 21-40. doi:10.1016/S0891-5520(02)00038-7

[4]   J. R. Bariola and K. S. Vyas, “Pulmonary Blastomycosis,” Seminars in Respiratory Critical Care Medicine, Vol. 32, No. 6, 2011, pp. 745-753. doi:10.1055/s-0031-1295722

[5]   J. A. McKinnell and P. G. Pappas, “Blastomycosis: New Insights into Diagnosis, Prevention, and Treatment,” Clinical Chest Medicine, Vol. 30, No. , 2009, pp. 227-239. doi:10.1016/j.ccm.2009.02.003

[6]   M. Saccente and G. L. Woods, “Clinical and Laboratory Update on Blastomycosis,” Clinical Microbiology Reviews, Vol. 23, No. 2, 2010, pp. 367-381. doi:10.1128/CMR.00056-09

[7]   J. A. Smith and C. A. Kauffman, “Pulmonary Fungal Infections,” Respirology, Vol. 17, No. 6, 2012, pp. 913-926.

[8]   J. A. Smith and C. A. Kauffman, “Blastomycosis,” Proceedings of the American Thoracic Society, Vol. 7, No. 3, 2010, pp. 173-180. doi:10.1513/pats.200906-040AL

[9]   J. E. Cutler, G. S. Deepe Jr. and B. S. Klein, “Advances in Combating Fungal Diseases: Vaccines on the Threshold,” Nature Review of Microbiology, Vol. 5, No. 1, 2007, pp. 13-18. doi:10.1038/nrmicro1537

[10]   R. W. Bradsher, “Detection of Specific Antibodies in Human Blastomycosis by Enzyme Immunoassay,” Southern Medical Journal, Vol. 88, No. 12, 1995, pp. 1256-1259. doi:10.1097/00007611-199512000-00013

[11]   C. A. Hage, T. E. Davis, L. Egan, M. Parker, D. Fuller, A. M. LeMonte, D. Durkin, P. Connelly, L. J. Wheat, D. Blue-Hindy and K. A. Knox, “Diagnosis of Pulmonary Histoplasmosis and Blastomycosis by Detection of Antigen in Bronchoalveolar Lavage fluid Using an Improved Second-Generation Enzyme-Linked Immunoassay,” Respiratory Medicine, Vol. 101, No. 1, 2007, pp. 43-47. doi:10.1016/j.rmed.2006.04.017

[12]   B. S. Klein and J. M. Jones, “Isolation, Purification, and Radiolabeling of a Novel Surface Protein on Blastomyces dermatitidis Yeasts to Detect Antibody in Infected Patients,” Journal of Clinical Investigation, Vol. 85, No. 1, 1990, pp. 152-161. doi:10.1172/JCI114406

[13]   K. S. Vyas, J. R. Bariola and R. W. Bradsher, “Advances in the Serodiagnosis of Blastomycosis,” Current Fungal Infection Reports, Vol. 2, No. 4, 2008, pp. 227-231. doi:10.1007/s12281-008-0033-z

[14]   B. S. Klein, R. A. Squires, J. K. Lloyd, D. R. Ruge and A. M. Legendre, “Canine Antibody Response to Blastomyces dermatitidis WI-1 Antigen,” American Journal of Veterinary Research, Vol. 61, No. 5, 2000, pp. 554-558. doi:10.2460/ajvr.2000.61.554

[15]   D. Spector, A. M. Legendre, J. Wheat, D. Bemis, B. Rohrbach, J. Taboada and M. Durkin, “Antigen and Antibody Testing for the Diagnosis of Blastomycosis in Dogs,” Journal of Veterinary Internal Medicine, Vol. 22, No. 4, 2008, pp. 839-843. doi:10.1111/j.1939-1676.2008.0107.x

[16]   J. F. Shurley, A. M. Legendre and G. M. Scalarone, “Blastomyes dermatitidis Antigen Detectioni in Urine Specimens from Dogs with Blastomycosis Using a Competitive Binding Inhibition ELISA,” Mycopathologia, Vol. 160, No. 2, 2005, pp. 137-142. doi:10.1007/s11046-005-3153-9

[17]   M. Durkin, L. Estok, D. Hospenthal, N. Crum-Cianflone, S. Swartzentruber, E. Hackett and L. J. Wheat, “Detection of Coccidioides Antigenemia Following Disssociation of Immune Complexes,” Clinical Vaccine Immunology, Vol. 16, No. 10, 2009, pp. 1453-1456. doi:10.1128/CVI.00227-09

[18]   J. R. Bariola, C. A. Hage, M. Durkin, E. Bensadoun, P. O. Gubbins, L. J. Wheat and R. W. Bradsher, “Detection of Blastomyces dermatitidis Antigen in Patients with Newly Diagnosed Blastomycosis,” Diagnostic Microbiology and Infectious Diseases, Vol. 69, No. 2, 2011, pp. 187-191. doi:10.1016/j.diagmicrobio.2010.09.015

[19]   P. Connolly, C. A. Hage, J. R. Bariola, E. Bensadoun, M. Rodgers, R. W. Bradsher and J. J. Wheat, “Blastomyces dermatitidis Antigen Detection by Quantitative Enzyme Immunoassay,” Clinical Vaccine Immunology, Vol. 19, No. 1, 2012, pp. 53-56. doi:10.1128/CVI.05248-11

[20]   D. R. Allton, R. G. Rivard, P. A. Connolly, S. McCall, M. M. Durkin, T. M. Boyd, J. P. Flanagan, L. J. Wheat and D. R. Hospenthal, “Detection of Latin American Strains of Histoplasma in a Murine Model by Use of a Commercially Available Antigen Test,” Clinical Vaccine Immunology, Vol. 17, No. 5, 2010, pp. 802-806.

[21]   C. A. Hage, J. A. Ribes, N. L. Wengenack, L. M. Baddour, M. Assi, D. S. McKinsey, K. Hammoud, D. Alapat, N. E. Babady, M. Parker, D. Fuller, A. Noor, T. E. Davis, M. Rodgers, P. A. Connolly, B. El Haddab and L. J. Wheat, “A Multicenter Evaluation of Tests for Diagnosis of Histoplasmosis,” Clinical Infectious Diseases, Vol. 53, No. 5, 2011, pp. 448-454. doi:10.1093/cid/cir435

[22]   S. M. Johnson and G. M. Scalarone, “Preparation and ELISA Evaluation of Blastomyces dermatitidis Yeast Phase Lysate Antigens,” Diagostic Microbiology and Infectious Diseases, Vol. 11, No. 2, 1989, pp. 81-86. doi:10.1016/0732-8893(88)90076-4

[23]   J. L. Bono, A. M. Legendre and G. M. Scalarone, “Detection of Antibodies and Delayed Hypersensitivity with Rotofor Preparative IEF fractions of Blastomyces dermatitidis Yeast Phase Lysate Antigens,” Journal of Medical and Veterinary Mycology, Vol. 33, No. 4, 1995, pp. 209- 214. doi:10.1080/02681219580000441

[24]   M. A. Fisher, J. L. Bono, R. O. Abuodeh, A. M. Legendre and G. M. Scalarone, “Sensitivity and Specificity of an Isoelectric Focusing Fraction of Blastomyces dermatitidis Yeast Lysate Antigen for the Detection of Canine Blastomycosis,” Mycoses, Vol. 38, No. 5-6, 1995, pp. 177-182. doi:10.1111/j.1439-0507.1995.tb00046.x

[25]   R. C. Axtell and G. M. Scalarone, “Serological Differences in Three Blastomyces dermatitidis Strains,” Mycoses, Vol. 45, No. 11-12, 2002, pp. 437-442.

[26]   C. M. Sestero and G. M. Scalarone, “Detection of IgG and IgM in Sera from Canines with Blastomycosis Using Eight Blastomyces dermatitidis Yeast Phase Lysate Antigens,” Mycopathologia, Vol. 162, No. 1, 2006, pp. 33-37. doi:10.1007/s11046-006-0028-7

[27]   C. M. Sestero and G. M. Scalarone, “Detection of the Surface Antigens BAD-1 and Alpha (1-3) Glucan in Six Different Strains of Blastomyces dermatitidis Using Monoclonal Antibodies,” Journal of Medical and Biological Science, Vol. 1, No. 1, 2006, pp. 1-7.

[28]   J. F. Shurley and G. M. Scalarone, “Isoelectric Focusing and ELISA Evaluation of a Blastomyces dermatitidis Human Isolate,” Mycopathologia, Vol. 164, No. 2, 2007, pp. 73-76. doi:10.1007/s11046-007-9033-8

[29]   W. O. Hatch and G. M. Scalarone, “Comparison of Colorimetric and Chemiluminescent ELISAs for the Detection of Antibodies to Blastomyces dermatitidis,” Journal of Medical and Biological Sciences, Vol. 3, No 1, 2009, pp. 1-6.

[30]   J. C. Wright, T. E. Harrild and G. M. Scalarone, “The Use of Isoelectric Focusing Fractions of Blastomyces dermatitidis for Antibody Detection in Serum Specimens from Rabbits Immunized with Yeast Lysate Antigens,” Open Journal of Veterinary Medicine, Vol. 2, No. 4, 2012, pp. 237-241. doi:10.4236/ojvm.2012.24038

[31]   H. B. Levine, G. M. Scalarone and S. D. Chaparas, “Preparation of Fungal Antigens and Vaccines: Studies on Coccidioides immitis and Histoplasma capsulatum,” Contributions to Microbiology and Immunology, Vol. 3, 1977, pp. 106-125.

[32]   G. M. Scalarone, H. B. Levine and S. D. Chaparas, “Delayed Hypersensitivity Responses of Experimental Animals to Histoplasmin from the Yeast and Mycelial Phases of Histoplasma capsulatum,” Infection and Immunity, Vol. 21, No. 3, 1978, pp. 705-713.

[33]   H. B. Levine, G. M. Scalarone, G. D. Campbell, R. C. Graybill and S. D. Chaparas, “Histoplasmin-CYL, A Yeast Phase Reagent in Skin Test Studies in Humans,” American Review of Respiratory Diseases, Vol. 119, No. 4, 1979, pp. 629-636.