The effects of antibiotics and surfactant addition on methionine
accumulation in the broth culture of three strains of methionine yeilding Bacilluscereus (B. cereus DS-13, B. cereus AS-9 and B. cereus RS-16) recovered from different soil ecovars in Owerri Nigeria
were investigated. Ampicillin and erythromycin neither stimulated growth nor
enhanced methionine production in Bacillus
cereus DS-13, while tetracycline and chloramphenicol stimulated growth and
enhanced methionine yield in Bacillus
cereus DS-13 and Bacillus cereus AS-9. Ampicllin had the highest stimulatory effect on growth and methionine
production in Bacillus cereus RS 16.
Tween 80 and palmitic acid stimulated growth and improved methionine
accumulation in the broth culture of all Bacillus strains while stearic acid did not improve yield in all Bacillus strains.
Cite this paper
K. Dike, I. Ekwealor and S. Eziuzor, "Influence of Antibiotics and Surfactants Addition on Growth and Methionine Productivity by Bacillus cereus," Advances in Microbiology, Vol. 3 No. 1, 2013, pp. 26-31. doi: 10.4236/aim.2013.31004.
 J. George, N. Pera, N. Phung, I. Leclercq, J. Y. Hou and G. Farrell, “Lipid Peroxidation, Stellate Cell Activation and Hepatic Fibrogenesis in a Rat Model of Chronic Steatohepatitis,” Journal of Hepatology, Vol. 39, No. 5, 2003, pp. 756-764. doi:10.1016/S0168-8278(03)00376-3
 K. K. Carroll and E. M. Kurowska, “Soy Consumption and Cholesterol Reduction: Review of Animal and Human Studies,” Journal of Nutrition, Vol. 125, No. 3, 1999, pp. 594S-597S.
 D. M. Townsend, K. D. Tew and H. Tapiero, “Sulfur Containing Amino Acids and Human Disease,” Biomedical Pharmacology, Vol. 58, No. 1, 2004, pp. 47-55.
 S. Kinoshita, S. Udaka and M. Shimono, “Amino Acid Fermentation. Production of L-Glutamic Acid by Various Microorganisms,” Journal of General Applied Microbiology, Vol. 3, No. 7, 1957, pp. 193-205.
 K. Soda, H. Tanaka and N. Esaki, “Amino Acids,” In: H. J. Rehm and G. Reed, Eds., Biotechnology, Pergamon Press, Oxford, 1983, pp. 479-530.
 H. Kase and K. Nakayama, “O-Acetylhomoserine as an Intermediate in Methionine Biosynthesis in Arthrobacter paraffineus, Corynebacterium glutamicum and Bacillus Species,” Agriculture and Biological Chemistry, Vol. 39, No. 3, 1975, pp. 687-693. doi:10.1271/bbb1961.39.687
 M. K. Chattopadhyay, D. Sengupta and S. Sengupta, “Fermentative Production of Methionine by 5-Bromouracil Resistant Mutants of Escherichia coli K-12,” Medica Science Research, Vol. 23, No. 23, 1995, pp. 775-776.
 F. Smekal, V. Bulant, E. Kindlova, M. Mazalova and S. Ulbert, “Production of L-Lysine Using Non-Standard Nitrogen Sources,” Kvansy Prum, Vol. 28, No. 3, 1982, pp. 9-40.
 K. S. Sen and S. P. Chatterjee, “Influence of B-Vitamins and Trace Elements on Lysine Production by Micrococcus varians 2fa,” Acta Biotechnology, Vol. 9, No. 1, 1986, pp. 63-67. doi:10.1002/abio.370090111
 C. J. Israilides, A. N. Weir and A. T. Bull, “Effect of Antibiotic on Lysine Production in Free and Immobilized Cells of Bacillus subtilis,” Applied Microbiology and Biotechnology, Vol. 32, No. 2, 1986, pp. 134-136.
 J. Konicek, F. Smekal and M. Konickova-Radochora, “Ef fect of tween 80 and Dimethyl Sulfoxide on Biosynthesis of Lysine in Regulatory Mutants of Corynebacterium glutamicum,” Folia Microbiology, Vol. 36, No. 6, 1991, pp. 587-589. doi:10.1007/BF02884043
 K. S. Dike and I. A. Ekwealor, “Production of L-Methionine by Bacillus cereus Isolated from Different Soil Ecovars in Owerri, South East Nigeria,” European Journal of Experimental Biology, Vol. 2, No. 2, 2012, pp. 311-314.
 K. S. Dike and I. A. Ekwealor, “Studies on Process and Physical Parameters for the Production of L-Methionine from Newly Isolated Bacillus cereus Strains,” Asian Journal of Biological Sciences, Vol. 5, No. 2, 2012, pp. 96-104. doi:10.3923/ajbs.2012.96.104
 G. I. Barrow and A. K. R Felthan, “Cowan and Steel’s Manual for the Identification of Medical Bacteria,” 3rd Edition, Cambridge University Press, Cambridge, 1993.
 R. E. Buchanan and N. E. Gibbon, “Bergey’s Manual of Determinative Bacteriology,” 8th Edition, Williams and Wilkins Co., Baltimore, 1974.
 J. P. Greenstein and M. W. Methionine, “Chemistry of the Amino Acid,” John Wiley & Sons, Chichester, 1961.
 K. Yamada, S. Kinoshita, T. Tsunoda and K. Aida, “The Microbial Production of Amino Acids,” John Wiley and sons, New York, 1972.
 A. L. Demain and J. Birnbaum, “Alteration of Permeability for the Release of Metabolites from the Microbial Cell,” Current Topics in Microbiology, Vol. 46, No. 2, 1968, pp. 1-26.
 D. Zaki, O. Galal, A. S. Hazino-Wahba, I. K. Morsi and A. E. Wakell, “Microbiological Production of Lysine,” Nutritional Research International, Vol. 26, No. 26, 1982, pp. 537-546.
 A. I. Ekwealor and J. A. N. Obetan, “Antibiotic and Surfactant Effects of Lysine Accumulation by Bacillus megaterium,” African Journal of Biotechnology, Vol. 7, No. 10, 2008, pp. 1550-1553.
 J. D. Desai and I. M Banat, “Microbial Production of Surfactants and Their Commercial Potential,” Microbiology and Molecular Biology Review, Vol. 61, No. 1, 1997, pp. 47-64.
 Q. Helmy, P. E. Suryatmana, V. Kardena and F. Wisjnuprapto, “Effect of Surfactant Addition on Oil Sludge Biodegradation by Petrofilic Bacteria,” The 3rd International Conference on Environmental Technology and Management, Bandung-Indonesia, October 2006, pp. 1-5.
 B. B. Ghosh and A. K Banerjee, “Production of Methionine and Glutamic Acid from n-Alkanes by Serratia marcescens Var. kiliensis,” Folia Microbiology, Vol. 31, No. 2, 1986, pp. 106-112. doi:10.1007/BF02926827
 V. R. William and E. A. Fieger, “Further Studies on Lipid Stimulation of Lactobacillus casei,” Journal of Biological Chemistry, Vol. 177, No. 2, 1949, pp. 739-744.
 O. Tosaka, Y. Yoshihara, S. Ikeda and K. Takinami, “Production of L-Lysine Fluoropyruvate Sensitive Mutants of Brevibacterium lactofermentum,” Agriculture and Biological Chemistry, Vol. 49, No. 5, 1985, pp. 1305-1312.
 H. Galbraith, T. B. Miller, A. M. Paton and J. K. Thompson, “Antibacterial Activity of Long Chain Fatty Acids and the Reversal with Calcium, Magnesium, Ergocalciferol and Cholesterol,” Journal of Applied Bacteriology, Vol. 34, No. 4, 1975, pp. 803-813.
 K. Takinami, O. Okada and T. Tsunoda, “Biochemical Effects of Fatty Acid and Its Derivatives on L-Glutamic Acid Fermentation I. Accumulation of L-Gluatamic Acid in the Presence of Sucrose Fatty Acid Ester,” Agriculture and Biological Chemistry, Vol. 27, No. 2, 1963, pp. 858-863.