ABSTRACT We previously studied the mechanism underlying the
adsorption of oral bacteria on the surfaces of dental prosthetic materials such
as ceramics and resins in vitro. The aim of the present study was to
examine bovine serum albumin (BSA) adsorption on crown
composite resin surfaces by means of zeta potential.
We measured the zeta potentials of resins alone, BSA alone, and resins after
BSA adsorption. Eight resins were pulverized into powders (300 - 1000 nm). All
experiments were conducted in 10 mM sodium chloride solution (pH 6.5). BSA
was dissolved in 10 mM NaCl with a concentration of 2.0 × 10-5 mol/l. An adsorption assay was performed for one hour at 37°C under
continuous rotation (6 rpm). The zeta potentials of both resins and BSA were
negative, with BSA itself less negative than the resins themselves as an
absolute value (p < 0.0001). The zeta potentials of seven resin surfaces
after BSA adsorption were significantly less negative than were those of the
resins without BSA adsorption (p < 0.0001). Eight resins were divided into
two classes based on the size of the surface potential difference between each
resin and the BSA. The difference in surface potential between the resins and
the BSA were small, leading to the theory that particles with identical charges
repulse each other, and the amounts of adsorbed BSA on these resins might be
less. On the other, when the differences between the other resins and BSA are
large, so that the repulsive force between two nonidentical particles becomes
zero and an attractive force might be generated, then more BSA might be
adsorbed on those resins. Therefore, the zeta potentials were affected by BSA
adsorption and became less negative. These results suggested that electrostatic
interactions play an important role in the adsorption of BSA on resin surfaces.
Cite this paper
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