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 AJPS  Vol.4 No.3 , March 2013
Metabolites from Resistant and Susceptible Pinus thunbergii after Inoculation with Pine Wood Nematode
Abstract: Pine wilt disease (PWD), which is caused by pine wood nematodes (PWN), is one of the most serious forest diseases worldwide. To clarify the mechanism of resistance to PWD, we compared metabolites from resistant and susceptible Japanese black pine (Pinus thunbergii) families after inoculation with PWN. After 2 weeks to 1 month post inoculation, the number of PWN dramatically increased in susceptible plants, but not in resistant plants. At this PWN-proliferation phase, ethyl acetate soluble fractions extracted from PWN-inoculated plants were analyzed by gas chromatogramphy-mass spectrometry (GC-MS). Although most compounds were qualitatively and quantitatively similar between resistant and susceptible plants, resistant plants accumulated 2.0-fold more linoleic acid (LA) than susceptible plants. On the other hand, benzoic acid (BA) was barely detected in resistant plants, but it accumulated in susceptible plants as the number of PWN increased. Susceptible plants contained greater levels of the nematicidal compounds pinosylvin and pinosylvin monomethyl ether, compared with resistant plants. These results suggested that LA is involved in the resistance reaction against PWN-proliferation, and that BA could be a good biomarker for PWD.
Cite this paper: F. Zhang, J. Kajiwara, Y. Mori, M. Ohira, Y. Tsutsumi and R. Kondo, "Metabolites from Resistant and Susceptible Pinus thunbergii after Inoculation with Pine Wood Nematode," American Journal of Plant Sciences, Vol. 4 No. 3, 2013, pp. 512-518. doi: 10.4236/ajps.2013.43065.
References

[1]   T. Kiyohara and Y. Tokushige, “Inoculation Experiments of a Nematode, Bursaphelenchus spp., onto Pine Trees,” Journal of the Japanese Forest Society, Vol. 53, No. 7, 1971, pp. 210-218.

[2]   B. Yang and Q. Wang, “Distribution of the Pinewood Nematode in China and Susceptibility of Some Chinese and Exotic Pines to the Nematode,” Canadian Journal of Forest Research, Vol. 19, No. 12, 1989, pp. 1527-1530. doi:10.1139/x89-232

[3]   C. K. Yi, B. H. Byun, J. D. Park, S. I. Yang and K. H. Chang, “First Finding of the Pine Wood Nematode, Bursaphelenchus Xylophilus (Steiner et Buhrer) Nickle and Its Insect Vector in Korea,” The Research Reports of the Forestry Research Institute, Vol. 38, 1989, pp. 141-149.

[4]   Y. Kishi, “The Pine Wood Nematode and the Japanese Pine Sawyer,” Thomas Company, Tokyo, 1995, p. 302.

[5]   J. C. Adams and A. L. Morehart, “Decline and Death of Pinus spp. in-Delaware Caused by Bursaphelenchus xylophilus,” Journal of Nematology, Vol. 14, No. 3, 1982, pp. 383-385.

[6]   L. D. Dwinell, “Relative Susceptibilities of Five Pine Species to Three Populations of the Pinewood Nematode,” Plant Disease, Vol. 69, No. 5, 1985, pp. 440-442.

[7]   K. Knowles, Y. Beaubien, M. J. Wingfield, F. A. Baker and D. W. French, “The Pinewood Nematode New in Canada,” The Forestry Chronicle, Vol. 59, 1983, p. 40.

[8]   F. A. Khan, “The Pine Wilt Disease Caused by Bursaphelenchus xylophilus in Nigeria,” Pakistan Journal of Nematology, Vol. 9, 1991, pp. 57-59.

[9]   L. D. Dwinell, “First Report of Pinewood Nematode (Bursaphelenchus xylophilus) in Mexico,” Plant Disease, Vol. 77, No. 8, 1993, p. 846. doi:10.1094/PD-77-0846A

[10]   M. M. Mota, H. Braasch, M. A. Bravo, A. C. Penas, W. Burgermeister, K. Metge and E. Sousa, “First Report of Bursaphelenchus xylophilus in Portugal and in Europe,” Nematology, Vol. 1, No. 7, 1999, pp. 727-734. doi:10.1163/156854199508757

[11]   L. Robertson, S. C. Arcos, M. Escuer, R. S. Merino, G. Esparrago, A. Abellera and A. Navas, “Incidence of the Pinewood Nematode Bursaphelenchus xylophilus Steiner & Buhrer, 1934 (Nickle, 1970) in Spain,” Nematology, Vol. 13, No. 6, 2011, pp. 755-757.

[12]   Y. Fujimoto, T. Toda, K. Nishimura, H. Yamate and S. Fuyuno, “Breeding Project on Resistance to Pine-Wood Nematode—An Outline of the Research and the Achieve- ment of Project for Ten Years,” Bulletin of the Forest Tree Breeding Institute, Vol. 7, 1989, pp. 1-84.

[13]   T. Toda and S. Kurinobu, “Realized Genetic Gains Observed in Progeny Tolerance of Selected Red Pine (Pinus densiflora) and Black Pine (P. thunbergii) to Pine Wilt Disease,” Silvae Genetica, Vol. 51, No. 1, 2002, pp. 42-44.

[14]   S. Goto, F. Miyahara and Y. Ide, “Contribution of Pollen Parents to the Nematode-Resistance in Seedlings of Japanese Black Pine,” Journal of the Japanese Forest Society, Vol. 84, No. 1, 2002, pp. 45-49.

[15]   Y. Mori, F. Miyahara and S. Goto, “Improving the Pine Wilt Disease Resistance of Cutting-Propagated Japanese Black Pine Plantlet Populations by Inoculating Ortets with Pinewood Nematodes,” Journal of the Japanese Forest Society, Vol. 88, No. 3, 2006, pp. 197-201. doi:10.4005/jjfs.88.197

[16]   Y. Mori, F. Miyahara and S. Goto, “The Effect of Rooted Cutting Propagation of Non-Damaged Japanese Black Pine through the Inoculation Test with the Pinewood Nematode on Nematode-Resistant Plant Production,” Proceeding of the International Symposium “Pine Wilt Disease: A Worldwide Threat to Forest Ecosystems”, Lisbon, 10-14 July 2006, pp. 52-53.

[17]   M. Ohira, F. Miyahara, Y. Mori, M. Okawa, J. Miyazaki, S. Masaki, K. Yoshimoto, Y. Sasaki, Y. Yamada, Y. Mitsugi, T. Tagami, T. Koyama, M. Miyazato, M. Tobase, K. Kuroda, M. Okamura, K. Matsunaga and S. Shiraishi, “Development of a Novel Cutting-Production System for Second-Generation Pinus thunbergii Resistant to Pine Wilt Disease,” Forest Tree Breeding, Vol. 235, 2010, pp. 1-5.

[18]   P. J. Bedker, M. J. Wingfield and R. A. Blanchette, “Pathogenicity of Bursaphelenchus xylophilus on Three Species of Pine,” Canadian Journal of Forest Research, Vol. 17, No. 1, 1987, pp. 51-57. doi:10.1139/x87-010

[19]   H. Oku, T. Shiraishi and K. Chikamatsu, “Active Defense as a Mechanism of Resistance in Pine Against Pine Wilt Disease,” Annals of the Phytopathological Society of Japan, Vol. 55, No. 5, 1989, pp. 603-608. doi:10.3186/jjphytopath.55.603

[20]   K. Kuroda, “Inhibiting Factors of Symptom Development in Several Japanese Red Pine (Pinus densiflora) Families Selected as Resistant to Pine Wilt,” Journal of Forest Research, Vol. 9, No. 3, 2004, pp. 217-224. doi:10.1007/s10310-004-0076-0

[21]   E. Kawaguchi, “Relationship between the Anatomical Characteristics of Cortical Resin Canals and Migration of Bursaphelenchus xylophilus in Stem Cuttings of Pinus thunbergii Seedlings,” Journal of the Japanese Forest Society, Vol. 88, No. 4, 2006, pp. 240-244. doi:10.4005/jjfs.88.240

[22]   Y. Mori, F. Miyahara, Y. Tsutsumi and R. Kondo, “Relationship between Resistance to Pine Wilt Disease and the Migration or Proliferation of Pine Wood Nematodes,” European Journal of Plant Pathology, Vol. 122, No. 4, 2008, pp. 529-538. doi:10.1007/s10658-008-9321-2

[23]   T. Suga, S. Ohta, K. Munesada, N. Ide, M. Kurokawa, M. Shimizu and E. Ohta, “Endogenous Pine Wood Nematicidal Substances in Pines, Pinus massoniana, P. strobus and P. palustris,” Phytochemistry, Vol. 33, No. 6, 1993, pp. 1395-1401. doi:10.1016/0031-9422(93)85098-C

[24]   F. Hanawa, T. Yamada and T. Nakashima, “Phytoalexins from Pinus strobus Bark Infected with Pinewood Nematode, Bursaphelenchus xylophilus,” Phytochemistry, Vol. 57, No. 2, 2001, pp. 223-228. doi:10.1016/S0031-9422(00)00514-8

[25]   N. Kuramoto, M. Ohira, M. Okamura, Y. Hiraoka, T. Taniguchi, M. Kashiwagi, Y. Inoue and Y. Fujisawa, “Genetic Analysis of Pine Wilt Disease-Resistance Using Interbreeding Families in Japanese Black Pine,” 116th the Japanese Forest Society Congress, Hokkaido, 27-30 March 2005, p. 649.

[26]   K. Kuroda, M. Ohira, M. Okamura and Y. Fujisawa, “Mi- gration and Population Growth of the Pine Wood Nematode (Bursaphelenchus xylophilus) Related to the Symptom Development in the Seedlings of Japanese Black Pine (Pinus thunbergii) Families Selected as Resistant to Pine Wilt,” Journal of the Japanese Forest Society, Vol. 89, No. 4, 2007, pp. 241-248. doi:10.4005/jjfs.89.241

[27]   G. Thorne, “Principles of Nematology,” McGraw-Hill Book Company Inc., New York, 1961, p. 553.

[28]   K. Kawazu, “Change in Constituents of Pine Wood by Infection of Pine Wood Nematode,” Nippon Nogeikagaku Kaishi, Vol. 64, No. 7, 1990, pp. 1262-1264. doi:10.1271/nogeikagaku1924.64.1262

[29]   T. Kohno, K. Togashi and N. Fukamiya, “The Nematocidal Activity and the Structure-Activity Relationships of Stilbenes,” Natural Product Research, Vol. 21, No. 7, 2007, pp. 606-615. doi:10.1080/14786410701369730

[30]   K. Kuroda, T. Yamada, K. Mineo and H. Tamura, “Effects of Cavitation on the Development of Pine Wilt Disease Caused by Bursaphelenchus xylophilus,” Annals of the Phytopathological Society of Japan, Vol. 54, No. 5, 1988, pp. 606-615. doi:10.3186/jjphytopath.54.606

[31]   N. Kuramoto, M. Sasaki, M. Okamura, Y. Hiraoka, M. Kashiwagi, Y.Inoue and Y. Fujisawa, “Comparison of Symptom Development of Pine Wilt Disease in Half-Sib Progeny of the Tanabe-t54 Open Family Line,” Kyushu Journal of Forest Research, Vol. 57, 2004, pp. 228-229.

[32]   I. S. Hwang and B. K. Hwang, “The Pepper 9-Lipoxygenase Gene CaLOX1 Functions in Defense and Cell Death Responses to Microbial Pathogens,” Plant Physiology, Vol. 152, No. 2, 2010, pp. 948-967. doi:10.1104/pp.109.147827

[33]   M. Nose and S. Shiraishi, “Comparison of the Gene Expression Profiles of Resistant and Non-Resistant Japanese Black Pine Inoculated with Pine Wood Nematode Using a Modified Long SAGE Technique,” Forest Pathology, Vol. 41, No. 2, 2011, pp. 143-155. doi:10.1111/j.1439-0329.2010.00646.x

[34]   H. Iwahoria and K. Futai, “Lipid Peroxidation and Ion Exudation of Pine Callus Tissues Inoculated with Pinewood Nematodes,” Japanese Journal of Nematology, Vol. 23, No. 2, 1993, pp. 79-89.

[35]   T. Ueda, H. Oku, K. Tomita, K. Sato and T. Shiraishi, “Isolation, Identification, and Bioassay of Toxic Compounds from Pine Tree Naturally Infected by Pine Wood Nematode,” Annals of the Phytopathological Society of Japan, Vol. 50, No. 2, 1984, pp. 166-175. doi:10.3186/jjphytopath.50.166

[36]   H. Shin, H. Lee, K.-S. Woo, E.-W. Noh, Y.-B. Koo and K.-J. Lee, “Identification of Genes Upregulated by Pinewood Nematode Inoculation in Japanese Red Pine,” Tree Physiology, Vol. 29, No. 3, 2009, pp. 411-421. doi:10.1093/treephys/tpn034

 
 
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