Back
 AJPS  Vol.4 No.3 , March 2013
Chemically Induced Mutants of Brassica oleracea var. botrytis Maintained Stable Resistance to Drought and Salt Stress after Regeneration and Micropropagation
Abstract: Investigation was made to confirm the stability of drought and salt stress tolerance in cauliflower (Brassica oleracea var.botrytis) mutants after regeneration and micropropagation. The N-nitroso-N-ethyleurea (NEU) and N-nitroso-N-methylurea (NMU) induced mutants of cauliflower were created and screened for drought and salt stress tolerance. The highly tolerant mutants were selected, regenerated by tissue culture techniques, screened again for drought and salt tolerance under in-vitro and in-vivo conditions, correlated the response of in-vitro and in-vivo plants within a clone. Free proline levels in clones were correlated with stress tolerance. Results confirmed the persistence of mutations in clones with enhanced resistance levels to stresses over control plants. The regenerated in-vitro and in-vivo plants within a clone showed a positive significant correlation for drought (R2 = 0.663) and salt (R2 = 0.647) resistance that confirms the stability of mutation in clones after generations. Proline showed a positive and significant correlation with drought (R2 = 0.524) and salt (R2 = 0.786) tolerance. Conclusively, drought and salt resistance can be successfully enhanced in cauliflower by chemical mutagenesis. Further molecular analysis is recommended to study these mutants.
Cite this paper: F. Hadi and M. Fuller, "Chemically Induced Mutants of Brassica oleracea var. botrytis Maintained Stable Resistance to Drought and Salt Stress after Regeneration and Micropropagation," American Journal of Plant Sciences, Vol. 4 No. 3, 2013, pp. 498-507. doi: 10.4236/ajps.2013.43063.
References

[1]   J. S. Boyer, “Plant Productivity and Environment,” Science, Vol. 218, No. 4571, 1982, pp. 443-448. doi:10.1126/science.218.4571.443

[2]   E. A. Bray, “Genes Commonly Regulated by Water- Deficit Stress in Arabidopsis Thaliana,” Journal of Experimental Botany, Vol. 55, No. 407, 2004, pp. 2331-2341. doi:10.1093/jxb/erh270

[3]   V. Shubha and K. T. Akhilesh, “Emerging Trends in the Functional Genomics of the Abiotic Stress Response in Crop Plants,” Plant Biotechnology Journal, Vol. 5, No. 3, 2007, pp. 361-380.

[4]   M. Shilpi and T. Narendra, “Cold, Salinity and Drought Stresses: An Overview,” Archives of Biochemistry and Biophysics, Vol. 444, No. 2, 2005, pp. 139-158.

[5]   J. Zhang, N. Klueva, Z. Wang, R. Wu, T. David and H. Nguyen, “Genetic Engineering for Abiotic Stress Resistance in Crop Plants,” In Vitro Cellular and Developmental Biology—Plant, Vol. 36, No. 2, 2000, pp. 108-114.

[6]   H. Brunner, “Radiation Induced Mutations for Plant Selection,” Plant Breeding Unit Joint FAO/IAEA Programme IAEA Laboratories, Seibersdorf, 1995.

[7]   A. Charlotte, “Mutation Research: Problems, Results, and Perspectives,” Trowbridge, 1976.

[8]   International Atomic Energy Agency, “Manual on Mutation Breeding,” Technical Report Series No. 119, 2nd Edition, Vienna, 1977.

[9]   I. Negrutu, “In-Vitro Mutagenesis,” In: P. Dix, Ed., Plant Cell Line Selection, VCH Publishers, Cambridge, 1990.

[10]   R. Mittler, “Abiotic Stress, the Field Environment and Stress Combination,” Trends in Plant Science, Vol. 11, No. 1, 2006, pp. 15-19. doi:10.1016/j.tplants.2005.11.002

[11]   V. Babu and T. N. Henry, “Understanding Regulatory Networks and Engineering for Enhanced Drought Tolerance in Plants,” Current Opinion in Plant Biology, Vol. 9, No. 2, 2006, pp. 189-195. doi:10.1016/j.pbi.2006.01.019

[12]   A. R. Kemble and H. T. MacPherson, “Liberation of Amino Acids in Perennial Rye Grass during Wilting,” Biochemical Journal, Vol. 58, No. 1, 1954, pp. 46-59.

[13]   W. B. Christopher, “Cancer Preventive Properties of Varieties of Brassica oleracea: A Review,” The American Journal of Clinical Nutrition, Vol. 59, No. 5, 1994, pp. 166S- 170S.

[14]   S. Tossaint, “History of Food,” Blackwell Publishing, Hoboken 1994.

[15]   M. P. Fuller, E. M. R. Metwali, M. H. Eed and A. J. Jellings, “Evaluation of Abiotic Stress Resistance in Mutated Populations of Cauliflower (Brassica oleracea var. botrytis),” Plant Cell, Tissue and Organ Culture, Vol. 86, No. 2, 2006, pp. 239-248. doi:10.1007/s11240-006-9112-4

[16]   M. Kieffer, N. Simkins, M. P. Fuller and A. J. Jellings, “A Cost Effective Protocol for In-Vitro Mass Propagation of Cauliflower,” Plant Science, Vol. 160, No. 5, 2001, pp. 1015-1024. doi:10.1016/S0168-9452(01)00347-8

[17]   M. P. Fuller and M. Eed, “The Development of Multiple Stress-Resistance Cauliflower Using Mutagenesis in Conjunction with a Micro-Shoot Tissue Culture Technique,” Acta Horticulturae, Vol. 618, 2003, pp. 71-76.

[18]   M. Kieffer, M. P. Fuller and A. J. Jellings, “Rapid Mass Production of Cauliflower Propagule from Fractionated and Graded Curd,” Plant Science, Vol. 107, No. 2, 1995, pp. 229-235. doi:10.1016/0168-9452(95)04110-G

[19]   T. Murashige and K. F. Skoog, “Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures,” Physiologia Plantarum, Vol. 15, No. 3, 1962, pp. 473-497. doi:10.1111/j.1399-3054.1962.tb08052.x

[20]   L. S. Bates, R. P. Waldren and I. O. Teare, “Rapid Determination of Free Proline for Water Stress Studies,” Plant Soil, Vol. 39, No. 1, 1973, pp. 205-207. doi:10.1007/BF00018060

[21]   R. A. Fisher and Y. Frank, “Statistical Tables for Biological, Agricultural and Medical Research,” 3rd Edition, W. C. Edinburgh Tweeddale Court, London, 1948.

[22]   M. Ashraf and P. Harris, “'Potential Biochemical Indicators of Salinity Tolerance in Plants,” Plant Science, Vol. 166, No. 1, 2004, pp. 3-16. doi:10.1016/j.plantsci.2003.10.024

[23]   M. Ashraf and T. McNeilly, “Salinity Tolerence in Brassica Oilseeds,” Critical Reviews in Plant Sciences, Vol. 23, No. 2, 2004, pp. 157-174. doi:10.1080/07352680490433286

[24]   S. F. Chandler and T. A. Thorpe, “Characterization of Growth, Water Relations, and Proline Accumulation in Sodium Sulfate Tolerant Callus of Brassica napus L. cv Westar (Canola),” Plant Physiology, Vol. 84, No. 1, 1987, pp. 106-111. doi:10.1104/pp.84.1.106

[25]   G. Rajasheker, D. Palmquist and C. A. Ledbetter, “In-Vitro Screening Procedure for Osmotic Tolerance in Prunus,” Plant Cell, Tissue and Organ Culture, Vol. 41, No. 2, 1995, pp. 159-164.

[26]   S. Grezesiak, W. Filek, D. Skrudilk and B. Niziol, “Screening for Drought Tolerance: Evaluation of Seeds Germination and Seedling Growth for Drought Resistance in Legume Plants,” Journal of Agronomy and Crop Science, Vol. 177, No. 4, 1996, pp. 245-252. doi:10.1111/j.1439-037X.1996.tb00242.x

[27]   S. Y. Sadighian and N. Yavari, “Effect of Water Stress on Germination and Early Seedling Growth in Sugar Beet,” Journal of Agronomy and Crop Science, Vol. 190, No. 2, 2004, pp. 138-144. doi:10.1111/j.1439-037X.2004.00087.x

[28]   J. C. Diaz-Perez, K. A. Shackel and E. G. Sutter, “Relative Water Content and Water Potential of Tissue-Cultured Apple Shoots under Water Deficits,” Journal of Experimental Botany, Vol. 46, No. 282, 1995, pp. 111-118. doi:10.1093/jxb/46.1.111

[29]   A. Kumar, P. Singh, D. P. Singh, H. Sigh and H. C. Sharma, “Differences in Osmo-Regulation in Brassica Species,” Annals of Botany, Vol. 54, No. 4, 1984, pp. 537-541.

[30]   A. Blum and C. Y. Sallivan, “The Comparative Drought Resistance of Landraces of Sorghum and Miller from Dry and Humid Regions,” Annals of Botany, Vol. 57, No. 6, 1986, pp. 835-846.

[31]   R. A. Moinuddin, K. D. Fischer and M. P. Renolds, “Osmotic Adjustment in Wheat in Relation to Grain Yeild under Water Deficit Environments,” Agronomy Journal, Vol. 97, 2005, pp. 1062-1071.

[32]   A. Kumar and J. Elston, “Genotypic Differences in Leaf Water Relations between Brassica juncea and Brassica napus,” Annals of Botany, Vol. 70, No. 1, 1992, pp. 3-9.

[33]   R. V. Kingsburry, E. Epstein and R. W. Pearcy, “Physiological Responses to Salinity in Selected Lines of Wheat,” Plant Physiology, Vol. 74, No. 2, 1984, pp. 417-423. doi:10.1104/pp.74.2.417

[34]   Y. Gibon, R. Sulpice and F. Larther, “Proline Accumulation in Canola Leaf Discs Subjected to Osmotic Stress Is Related to Loss of Chlorophylls and to Decrease of Mitrochondria Activity,” Physiologia Plantarum, Vol. 110, No. 4, 2000, pp. 469-476. doi:10.1111/j.1399-3054.2000.1100407.x

[35]   J. Huang and R. Redmann, “Responses of Growth, Morphology, and Anatomy to Salinity and Calcium Supply in Cultivated and Wild Barley,” Canadian Journal of Botany, Vol. 73, No. 12, 1995, pp. 1859-1866. doi:10.1139/b95-198

[36]   R. K. Jain and S. Jain, “In-Vitro Selection for Salt Tolerence in Brassica juncea L. Using Cotyledon Explants, Callus and Cell Suspension Cultures,” Annals of Botany, Vol. 67, No. 6, 1991, pp. 517-519.

[37]   R. E. A. Moghaieb, H. Saneoka and K. Fujita, “Effect of Salinity on Osmotic Adjustment, Glycinbetaine Accumulation and the Betaine Aldehyde Hydrogenase Gene Expression in Two Halophytic Plants, Salicornia europaea and Suaeda maritime,” Plant Science, Vol. 166, No. 5, 2004, pp. 1345-1349. doi:10.1016/j.plantsci.2004.01.016

[38]   R. W. Kingsburry and E. Epstein, “Salt Sensitivity in Wheat, a Case for Specific Ion Toxicity,” Plant Physiology, Vol. 80, No. 3, 1986, pp. 651-654. doi:10.1104/pp.80.3.651

 
 
Top