Back
 AJPS  Vol.4 No.2 , February 2013
Acclimatization of in Vitro Propagated Pineapple (Ananas comosuss (L.), var. Smooth cayenne) Plantlets to ex Vitro Condition in Ethiopia
Abstract: Pineapple (Ananas comosuss, var. Smooth cayenne), which is a popular tropical fruit, is propagated vegetatively. Conventional propagation alone does not provide clean and adequate planting material demanded in Ethiopia. Recently, in vitro multiplication has become a promising technique for large-scale production. However, the acclimatization to the external environment procedure impedes the efficiency, which needs carefully optimized acclimatization techniques. We report optimized acclimatization procedures following first- and second-stage hardening methods for in vitro pineapple plantlets. Primarily, Jiffy-7 peat pellet allowed growing plants vigorously and provided above 8% survival rate over soil mix. Nevertheless, in Ethiopia, soil mix is cheaper and locally accessible. The primarily acclimatized plantlets are needed to be hardened further for better establishment and survival in the field. Black polybag and polysleeve pots filled with soil mix were evaluated in the greenhouse. A significant difference was obtained between pots for number of roots and substrate weight. Polybags had higher root number than polysleeves and saved about 27% of substrates per plant, which is a reduction of 25% of total transportation cost. Hence, the soil mix and polybags were found to be preferable over substrates and pots, for subsequent in vitro pineapple acclimatization.
Cite this paper: A. Mengesha, B. Ayenew and T. Tadesse, "Acclimatization of in Vitro Propagated Pineapple (Ananas comosuss (L.), var. Smooth cayenne) Plantlets to ex Vitro Condition in Ethiopia," American Journal of Plant Sciences, Vol. 4 No. 2, 2013, pp. 317-323. doi: 10.4236/ajps.2013.42042.
References

[1]   G. C. D’Eeckenbrugge and F. Leal, “Morphology, Anatomy and Taxonomy,” In: D. P. Bartholomew, R. E. Paull, and K. G. Rohrbach, Eds., The Pineapple: Botany, Production and Uses, CAB International, Wallingford, 2003, pp. 13-32. doi:10.1079/9780851995038.0013

[2]   E. Edossa, “Spice Research Achievements and Experiences,” Research Report No. 33, IAR, Addis Ababa, 1998, pp. 16-19.

[3]   E. Firoozabady and N. Gutterson, “Cost-Effective in Vitro Propagation Methods for Pineapple,” Plant Cell Report, Vol. 21, 2003, pp. 844-850. doi:10.1007/s00299-003-0577-x

[4]   Z. Abebe, W, Tefera, M. Fellipe, A. Teressa and A. Mengesha, “In Vitro Multiplication of Pineapple (Ananas comosuss (L.)) and Cardamom (Elletaria cardamomum) in Ethiopia,” Proceeding of the Second Biennial Conference of Ethiopian Horticultural Science Society, Addis Ababa, 22-23 January, 2003, pp. 9-18.

[5]   J. B. Teixeira, A. R. R. Cruz, F. R. Ferreira and J. R. Cabral, “Biotechnology Applied to Seedling Production: Production of Pineapple Plantlets,” Science and Biotechnology Development, Vol. 3, 2001, pp. 42-47.

[6]   J. Pospisilova, I. Ticha, P. Kadleaeek, D. Haisel and S. Plazakova, “Acclimatization of Micropropagated Plants to Ex Vitro Conditions,” Biologia Plantarum, Vol. 42, No. 4, 1999, pp. 481-497. doi:10.1023/A:1002688208758

[7]   G. Fila, J. Ghashghaie and G. Cornic, “Photosynthesis, Leaf Conductance and Water Relations of in Vitro Cultured Grapevine Rootstock in Relation to Acclimatization,” Biologia Plantarum, Vol. 102, No. 3, 1998, pp. 411-418. doi:10.1034/j.1399-3054.1998.1020309.x

[8]   A. S. Davis, K. Eggleston, J. R. Pinto and R. K. Dumroese, “Evaluation of Three Growing Media Substrates for Western Larch Seedling Production at the USDA Forest Service Coeur d’Alene Nursery,” In: R. K. Dumroese, L. E. Riley, Eds., National Proceedings: Forest and Conservation Nursery Associations-2008, Rocky Mountain Research Station, 2009, pp. 37-41.

[9]   S. R. Vasane and R. M. Kothari, “Optimization of Sec- ondary Hardening Process of Banana Plantlets (Mussa paradisiacal L. var. grand Nain),” Indian Journal of Biotechnology, Vol. 5, 2006, pp. 394-399.

[10]   L. E. B. Baldotto, M. A. Baldotto, L. P. Canellas, R. Bressan-Smith and F. L. Olivares, “Growth Promotion of Pineapple ‘Vitória’ by Humic Acids and Burkholderia spp. during Acclimatization,” Revista Brasileira de Ciência do Solo, Vol. 34, No. 5, 2010, pp.1593-1600. doi:10.1590/S0100-06832010000500012

[11]   B. Ayenew, T. Tadesse, E. Gebremariam, A. Mengesha and W. Tefera, “Efficient Use of Temporary Immersion Bioreactor (TIB) on Pineapple (Ananas comosus L.) Multiplication and Rooting Ability,” Journal of Microbiology, Biotechnology and Food Science, Vol. 2, No. 4, 2013, pp. 2456-2465.

[12]   B. Ayenew, A. Mengesha, T. Tadesse and E. Gebremariam, “Ensete ventricosum (Welw), Cheesman: A Cheap and Alternative Gelling Agent for pineapple (Ananas comosus var. Smooth Cayenne) in Vitro Propagation,” Journal of Microbiology, Biotechnology and Food Science, Vol. 2, No. 2, 2012, pp. 640-652.

[13]   B. Ayenew, W. Tefera and B. Kassahun, “In Vitro Propagation of Ethiopian Ginger (Zingiber officinale Rosc.) Cultivars: Evaluation of Explant Types and Hormone Combinations,” Africa Journal of Biotechnology, Vol. 11, No. 16, 2012, pp. 3911-3918.

[14]   D. Montgomery, “Design and Analysis of Experiments,” 6th Edition, John Wiley and Sons. Inc, New York, 2005, pp. 97-203.

[15]   SAS Institute, “SAS/STAT User’s Guide for Personal Computers, Release 8.01,” SAS Institute, Cary, 2001.

[16]   K. F. Salifu, M. A. Nicodemus, D. F. Jacobs and A. S. Davis, “Evaluating Chemical Indices of Media for Nursery Production of Quercus rubra Seedlings,” Horticultural Science, Vol. 41, No. 5, 2006, pp. 1342-1346.

[17]   D. W. Reed, “Water, Media, and Nutrition for Greenhouse Crops,” Ball Publishing Inc., Illinois, 1996.

[18]   M. Bergeron, “Peat,” Canadian Minerals Yearbook, Natural Resources, Ottawa, 37.1-37.8, 1994.

[19]   J. E. Wyatt and J. A. Mullins, “Production of Sweat Corn from Transplants,” Horticultural Science, Vol. 24, No. 6, 1989, p. 1039.

[20]   T. D. Landis and N. Morgan, “Growing Media Alternatives for Forest and Native Plant Nurseries,” In: R. K. Dumroese and L. E., Riley, Eds., National Proceedings: Forest and Conservation Nursery Associations-2008. Forest Service, Rocky Mountain Research Station, 2009, pp. 26-31.

[21]   M. Raviv, “Horticultural Uses of Composted Materials,” Acta Horticulture, Vol. 469, 1998, pp. 225-234. http://www.actahort.org/books/469/469_23.htm

[22]   S. C. Grossnickle, “Importance of Root Growth in Overcoming Planting Stress,” New Forests, Vol. 30, No. 2-3, 2005, pp. 273-294. doi:10.1007/s11056-004-8303-2

 
 
Top