OJBIPHY  Vol.3 No.1 A , February 2013
Error Corrected Sub-Monolayer Ellipsometry for Measurement of Biomolecular Interactions
Author(s) Udo Riss*
ABSTRACT

Already in 1946 Alexandre Rothen from the Rockefeller Institute for Medical Research, New York published the use of ellipsometry for the measurement of antigen-antibody interactions. And he found some effects that could not be explained by the assumption that the interaction of the molecules is only of chemical nature. 55 years later we started research on antibody-antigen interaction and found similar results. To make sure that these measurements are not produced by measurement artifacts, each component of our measurement technique is error analyzed and error corrected if necessary. With such type of error corrected instrumentation we found, that there must be an interaction between antigens and antibodies that is based on longitudinal electromagnetic waves, which are able to work through thin 7 nm membranes. A similar interaction is found for the virus-antigen interaction. Our measurement results are in contrast to the assumption that the antigen-antibody and antigen-virus interaction is only of chemical nature.


Cite this paper
U. Riss, "Error Corrected Sub-Monolayer Ellipsometry for Measurement of Biomolecular Interactions," Open Journal of Biophysics, Vol. 3 No. 1, 2013, pp. 76-85. doi: 10.4236/ojbiphy.2013.31A010.
References
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[2]   A. Rothen, “Immunological Reaction between Films of Antigen and Antibody Molecules,” JBC, 1946.

[3]   U. Riss, “Theory of Long Distance Interaction between Antibodies and Antigens,” European Biophysics Journal, Vol. 40, No. 8, 2011, pp. 987-1005.

[4]   U. Riss, “Zur Transmissionsellipsometrie Optisch Anisotroper Komponenten und Systeme,” Ph.D. Dissertation (Mechanical Engineering), University of Kassel, Kassel, 1988.

[5]   U. Riss and W. Holzapfel, “Properties and Characteristics of Optical Glass,” SPIE Proceedings, Vol. 970, 1988, pp. 48-61.

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[7]   Y. Chen, “Generation and Affinity Maturation of Fully Human Soluble VH Domain Antibodies and IgGs Using dsDNA Display,” Bit’s 4th Annual Congress of Antibodies, Peking, 2012.

[8]   U. Riss, “Theory of Long Distance Interaction between Antibodies and Antigens,” European Biophysics Journal, Vol. 40, No. 8, 2011, pp. 987-1005.

[9]   U. Riss, “Theory and Measurement of Long Distance Interaction between Antibodies and Antigens,” Bit’s 4th Annual Congress of Antibodies, Peking, 2012.

[10]   A. Schmiedl and S. Dübel, “Rekombinate Antikorper and Phagendisplay,” 2004. http://www.bbt.tu-bs.de/Biotech/SD/PDF/MolBioTech03Fin.pdf

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[12]   U. Riss, “Long Distance Interaction of Antibodies, Monoclonal Antibodies, Exploring Innovation in Therapeutic Antibody Development and Production,” London, 2012.

[13]   TED Pella Inc., 2013. http://www.tedpella.com/grids_html/silicon-nitride.htm#_21500_10

 
 
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