JACEN  Vol.2 No.1 , February 2013
Isolation and identification of alkaloids and anthocyanins from flower and bulb of Lycoris radiata using HPLC and LC-ESI-MS
Abstract: Three anthocyanins (cyanidin 3-diglucoside, cyanidin 3-sambubioside and cyanidin 3-glucoside) together with eleven known alkaloids (lycoricidine, hipppeastrine,O-Demethyllycoramin, lycoricidinol, galanthine, lycorine, lycorenine, lycoramine, galanthamine, homolycorine and pretazettine) were identified in the flower and bulb of Lycoris radiata using high-performance liquid chromatography coupled with UV detection and electrospray ionization mass spectrometry. Anthocyanins play a major role in protecting plant’s DNA from the UV spectrum of sunlight and also in attracting insects for the purpose of pollination. Thus, knowledge on the contents and types of anthocyanins of L. radiata will help to evaluate the adaptive evolution of flowers and provide useful information for the ornamental breeding.
Cite this paper: J. Chun, I. Jang, M. Arasu, N. Al-Dhabi, V. Duraipandiyan, D. Lee, S. Lee and S. Kim, "Isolation and identification of alkaloids and anthocyanins from flower and bulb of Lycoris radiata using HPLC and LC-ESI-MS," Journal of Agricultural Chemistry and Environment, Vol. 2 No. 1, 2013, pp. 22-26. doi: 10.4236/jacen.2013.21004.
References

[1]   Feng, T., Wang, Y.-Y., Su, J., Li, Y., Cai, X.-H. and Luo, X.-D. (2011) Amaryllidaceae alkaloids from Lycoris radiate. Helvetica Chimica Acta, 94, 178-183. doi:10.1002/hlca.201000176

[2]   Zahida, I., Habib, N., Syuntaro, H. and Yoshiharu, F. (2006) Plant growth inhibitory activity of Lycoris radiata Herb. and the possible involvement of lycorine as an allelochemical. Weed Biology and Management, 6, 221-227. doi:10.1111/j.1445-6664.2006.00217.x

[3]   Yosuke, T., Eri, K., Noriyuki, K., Mariko, K., Aki, I., Kazuhiko, O., Haruki, Y., Satoshi, O. and Hiromitsu, T. (2009) New lycorine-type alkaloid from Lycoris traubii and evaluation of antitrypanosomal and antimalarial activities of lycorine derivatives. Bioorganic and Medicinal Chemistry, 16, 10182-10189.

[4]   Mazza, G. and Miniati, E. (1992) Anthocyanins in fruits, vegetables, and grains. CRC Press, Boca Raton.

[5]   Kihara, M., Konishi, K., Xu, L. and Kobayashi, S. (1991) Alkaloidal constituents of the flowers of Lycoris radiata HERB (Amaryllidaceae). Chemical & Pharmaceutical Bulletin, 39, 1849-1853. doi:10.1248/cpb.39.1849

[6]   Zhong, J. (2005) Amaryllidaceae and Sceletium alkaloids (Review). Natural Product Reports, 22, 111-126. doi:10.1039/b316106b

[7]   Wu, X. and Prior, R.L. (2005) Systematic identification and characterization of anthocyanins by HPLC-ESI-MS/ MS in common foods in the United States: Fruits and berries. Journal of Agricultural Food Chemistry, 53, 2589- 2599. doi:10.1021/jf048068b

[8]   Giusti, M.M., Rodriguez-Saona, L.E., Griffin, D. and Wrolstad, R.E. (1999) Electrospray and tandem mass spectroscopy as tools for anthocyanin characterization. Journal of Agricultural Food Chemistry, 47, 4657-4664. doi:10.1021/jf981242+

[9]   Solovchenko, A.E. and Merzlyak, M.N. (2008) Screening of visible and UV radiation as a photoprotective mechanism in plants. Russian Journal of Plant Physiology, 55, 719-737.

[10]   Yoo, D.S., Choi, Y.H., Cha, M.R., Lee, B.H., Kim, S.-J., Yon, G.H., Hong, K.S., Jang, Y.S., Lee, H.S., Kim, Y.S., Ryu, S.Y. and Kang, J.S.(2011) HPLC-ELSD analysis of 18 platycosides from balloon flower roots (Platycodi Radix) sourced from various regions in Korea and geographical clustering of the cultivation areas. Food Chemistry, 129, 645-651. doi:10.1016/j.foodchem.2011.04.106

 
 
Top