ABC  Vol.3 No.1 , February 2013
PCR-based molecular markers linked to the leaf rust resistance gene Lr19 in different bread wheat cultivars
ABSTRACT

61 varieties of wheat collected in the gene fund of the Research Institute of Crop Husbandry were screened using SCAR-markers associated with the gene of resistance to brown leaf rust, Lr19. As a result of PCR analysis using SCS123 marker the 737 bp locus was detected in 48 genotypes. The expected fragment of the 688 bp was detected in 53 genotypes using the SCS253 marker. The results obtained using both markers indicate that the Lr19 gene is present on 7D chromosomes of 45 genotypes. The existence of the Lr19 gene has not been proven only for 5 from the 61 analyzed wheat genotypes.


Cite this paper
Huseynova, I. , Guliyeva, F. , Rustamova, S. and Aliyev, J. (2013) PCR-based molecular markers linked to the leaf rust resistance gene Lr19 in different bread wheat cultivars. Advances in Biological Chemistry, 3, 153-158. doi: 10.4236/abc.2013.31019.
References
[1]   Singh, R.P., Huerto-Espino, J. and Rajaram, S. (2000) Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathologica et Entomologica Hungarica, 35, 133-139.

[2]   Oelke, L.M. and Kolmer, J.A. (2004) Characterization of leaf rust resistance in hard red spring wheat cultivars. Plant Disease, 88, 1127-1133. doi:10.1094/PDIS.2004.88.10.1127

[3]   Mebrate, S.A., Oerke, E.C., Dehne, H.W. and Pillen, K. (2008) Mapping of the leaf rust resistance gene Lr38 on wheat chromosome arm 6DL using SSR markers. Euphytica, 162, 457-466. doi:10.1007/s10681-007-9615-z

[4]   McIntosh R.A., Wellings C.R. and Park R.F. (1995) Wheat rusts: An atlas of resistance genes. CSIRO Publications, Melbourne, 208. doi:10.1007/978-94-011-0083-0

[5]   Marasas, C.N., Smale, M. and Singh, R.P. (2003) The economic impact of productivity maintenance research: Breeding for leaf rust resistance in modern wheat. Agricultural Economics, 29, 253-263. doi:10.1111/j.1574-0862.2003.tb00162.x

[6]   Woxniak-Strzembicka, A. (2003) Wirulencja populacji Puccinia recondita f. sp. tritici w Polsce wlatach 1998-2001. Biuletyn Instytutu Hodowli i Aklimatyzacji Roslin, 230, 109-117.

[7]   Vanzetti, L.S., Campos, P., Demichelis, M., Lombardo, L.A., Aurelia, P.R., Vaschetto, L.M., Bainotti, C.T. and Helguera, M. (2011) Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers. Electronic Journal of Biotechnology, 14, 3. doi:10.2225/vol14-issue3-fulltext-14

[8]   Gupta, S.K., Charpe, A., Prabhu, K.V. and Haque, Q.M. (2006) Identification and validation of molecular markers linked to the leaf rust resistance gene Lr19 in wheat. Theoretical and Applied Genetics, 113, 1027-1036. doi:10.1007/s00122-006-0362-7

[9]   McIntosh, R.A., Dubcovsky, J., Rogers, W.J., Morris, C., Appels, R. and Xia, X.C. (2011) Catalogue of gene symbols for wheat: 2011 supplement. http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2011.pdf

[10]   Prins, R., Marais, G.F., Pretorius, Z.A., Janse, B.J.H. and Marais, A.S. (1997) A study of modified forms of the Lr19 translocation of common wheat. Theoretical and Applied Genetics, 95, 424-430. doi:10.1007/s001220050579

[11]   Tomar, S.M.S. and Menon, M.K. (1998) Adult plant response of nearisogenic lines and stocks of wheat carrying specific Lr genes against leaf rust. Indian Phytopathology, 51, 61-67.

[12]   Mesterhazy, A., Bartos, P., Goyeau, H., Niks, R.E., Csosz, M., Andersen, O., Casulli, F., Ittu, M., Jones, E., Manis terski, J., Manninger, K., Pasquini, M., Rubiales, D., Schachermayr, G., Strzembicka, A., Szunics, L., To dorova, M., Unger, O., Vanco, B., Vida, G. and Walther, U. (2000) European virulence survey for leaf rust in wheat. Agronomie, 20, 793-804. doi:10.1051/agro:2000104

[13]   McCallum, B.D. and Seto-Goh, P. (2003) Physiologic specialization of wheat leaf rust (Puccinia triticina) in Canada in 2000. Canadian Journal of Plant Pathology, 25, 91-97. doi:10.1080/07060660309507053

[14]   Miralles, D.J., Resnicoff, E. and Carretero, R. (2007) Yield Improvement associated with Lr19 translocation in wheat. In: Spiertz, J.H.J., Struik, P.C. and van Laar, H.H., Eds., Scale and Complexity in Plant Systems Research: Gene-Plant-Crop Relationships, Springer, Dordrecht, pp. 171-178. doi:10.1007/1-4020-5906-X_14

[15]   Sharma, D. and Knott, D.R. (1966) The transfer of leaf rust resistance from Agropyron to triticum by irradiation. Canadian Journal of Genetics and Cytology, 8, 137-143.

[16]   Sehgal, S.A., Tahir, R.A., Anwar, Z., Abbas, G., Kausar Nawaz Shah, M. and Zaman Khan Khattak, J. (2012) Molecular genetic characterization of rust in wheat genotypes. Asian Journal of Agricultural Sciences, 4, 337-340.

[17]   Huerta-Espino, J. and Singh, R.P. (1994) First report of virulence for wheat leaf rust gene Lr19 in Mexico. Plant Disease, 78, 640. doi:10.1094/PD-78-0640C

[18]   Knott, D.R. (1980) Mutation of a gene for yellow pigment link to Lr-19 in wheat. Canadian Journal of Genetics and Cytology, 22, 651-654.

[19]   Kim, N.S., Amstrong, K. and Knott, D.R. (1993) Molecular detection of lophopyrum chromatin in wheat lo phopyrum recombinants and their use in the physical mapping of chromosome 7D. Theoretical and Applied Genetics, 85, 561-567. doi:10.1007/BF00220914

[20]   Marais, G.F. and Marais, A.S. (1990) The assignment of a thinopyrum disticum (thunb) love derived translocation to the long arm of wheat chromosome 7D using endopeptidase polymorphism. Theoretical and Applied Genetics, 79, 182-186. doi:10.1007/BF00225949

[21]   Sibikeev, S.N., Kruprov, V.A., Voronina, S.A. and Elesin V.A. (1996) First report of leaf rust pathotypes virulent on highly effective Lr-genes transferred from Agropyron species to bread wheat. Plant Breeding, 115, 276-278. doi:10.1111/j.1439-0523.1996.tb00917.x

[22]   Roelfs, A.P. (1988) Genetic control of phenotypes in wheat stem rust. Annual Review of Phytopathology, 26, 351-367. doi:10.1146/annurev.py.26.090188.002031

[23]   Pink, D. (2002) Strategies using genes for non-durable disease resistance. Euphytica, 124, 227-236. doi:10.1023/A:1015638718242

[24]   Murray, M.G. and Thompson, W.F. (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8, 4321-4325. doi:10.1093/nar/8.19.4321

[25]   Williams, J.G., Kubelik, K.J., Livak, J.A. and Tingey, S.V. (1990) DNA polymorphisms amplified by arbitrary primers are useful genetic markers. Nucleic Acids Research, 18, 6531-6535. doi:10.1093/nar/18.22.6531

[26]   Tirishkin, L.G. (2006) Genetic control of the effective juvenile brown rust resistance of collection Triticum aestivum L. wheat samples. Genetics, 42, 377-384.

[27]   Dyck, P.L. and Sambroski, D.J. (1982) The inheritance of resistance to puccinia in a group of common wheat cultivars. Canadian Journal of Genetics and Cytology, 24, 2733-2783.

 
 
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