This study examined the effect of underground
water on reproduction- and growth-related hormones in blue gourami
males under non-reproductive and reproductive conditions. An increase in the
percentage of males building nests under the highest percentage of underground
water were compared to fish that maintained a lower percentage of underground water
in the first two days. The % Gonado-somatic index (GSI) of males building nests
was higher than non- reproductively active males in water containing the lowest
concentration of underground water. In non- reproductively active males, brain
gonadotropin releasing hormone 1 (GnRH1) and pituitary β subunit of gonadotropins (GtHs) and prolactin (PRL) mRNA levels
were significantly higher in males maintained in underground water. In
reproductively active males, mRNA levels of brain GnRH1, gonadotropin releasing
hormone 3 (GnRH3) and pituitary PRL mRNA levels were significantly higher than
males maintained in underground water. Thus, it is suggested that underground
water with high salinity and conductivity levels affects the gene expression
of repro- duction-related hormones; in reproductively active males, it
shortened the duration of nest-building by blue gourami males.
Cite this paper
Degani, G. and Levy, G. (2013) Underground water affects sexual behavior and gene expression of hormones related to reproduction in blue gourami males. Advances in Biological Chemistry, 3, 133-140. doi: 10.4236/abc.2013.31016.
 Dodd, J.M. and Sumpter, J.P. (1982) Reproductive cycles of cyclostomes, elasmobranchs and bony fishes. In: Lamming, G.E., Ed., Marshall’s Physiology of Reproduction, Churchill Living-stone, London, 1-126.
 Munro, A.D., Scott, A.P. and Lam, T.J. (1990) Reproductive seasonality in Teleosts: Environmental influences. CRC Press, Boca Raton.
 Drori, S., Michal, O.M., Levavi-Sivan, B. and Yaron, Z. (1994) Spawning induction in common carp (Cyprivtus carpio) using pituitary extract or GnRH superactive ana logue combined with metoclopramide: Analysis of hormone profile, progress of oocyte maturation and dependence on temperature. Aquaculture, 119, 393-407.
 Yaron, Z. (1969) Correlation between spawning, water temperature and thyroid activity in Acanthobrama terrae sanctae (Cyprinidae) of Lake Tiberias. General and Comperative Endocrinology, 12, 604-608.
 Yaron, Z., Bogomolnaya, A. and B., L. (1984) A calibrated carp pituitary extract as a spawning-inducing agent. Research on Aquaculture, 8 Edition, Europe Mariculture Society, Bredene.
 Degani, G. (1989) The effect of temperature, light, fish size and container size on breeding of Trichogaster tri chopterus. Israeli Journal of Aquaculture Bamidgeh, 41, 67-73.
 Holloway, A.C., Sheridan, M.A., Van der Kraak, G. and Leatherland, J.F. (1999) Correlations of plasma growth hormone with somatostatin, gonadal steroid hormones and thyroid hormones in rainbow trout during sexual re crudescence. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 123, 251 260. doi:10.1016/S0305-0491(99)00059-0
 Shimizu, A. (2003) Effect of photoperiod and temperature on gonadal activity and plasma steroid levels in a reared strain of the mummichog (Fundulus heteroclitus) during different phases of its annual reproductive cycle. General and Comparative Endocrinology, 131, 310-324.
 Hellqvist, A., Bornestaf, C., Borg, B. and Schmitz, M. (2004) Cloning and sequencing of the FSH-b and LH-b subunit in the three-spined stickleback, Gasterosteus aculeatus, and effects of photoperiod and temperature on LH-b and FSH-b mRNA expression. General and Com perative Endocrinolology, 135, 167-174.
 Soria, F.N., Strussmann, C.C.A. and Miranda, L.A. (2008) High water temperatures impair the reproductive ability of the pejerrey fish Odontesthes bonariensis: Effects on the hypophyseal-gonadal axis. Physiological and Bio chemical Zoology, 81, 898-905. doi:10.1086/588178
 Sumpter, J.P. (1997) Environmental control of fish re production: A different perspective. Fish Physiology and Biochemistry, 17, 25-31. doi:10.1023/A:1007782305962
 Seale, A.P., Riley, L.G., Leedom, T.A., Kajimura, S., Dores, R.M., Hirano, T. and Grau, E.G. (2002) Effects of environmental osmolality on release of prolactin, growth hor-mone and ACTH from the tilapia pituitary. General and Comparative Endocrinology, 128, 91-101.
 Breves, J.P., Fox, B.K., Pierce, A.L., Hirano, T. and Grau, E.G. (2010) Gene expression of growth hormone family and glucocorticoid receptors, osmosensors, and ion trans porters in the gill during seawater acclimation of Mozam bique tilapia, Oreochromis mossambicus. Journal of Ex perimental Zoology Part A: Ecological Genetics and Physiology, 313, 432-441. doi:10.1002/jez.613
 Fox, B.K., Breves, J.P., Davis, L.K., Pierce, A.L., Hirano, T. and Grau, E.G. (2010) Tissue-specific regulation of the growth hormone/insulin-like growth factor axis during fasting and refeeding: Importance of muscle expression of IGF-I and IGF-II mRNA in the tilapia. General and Comperative Endocrinology, 166, 573-580.
 Degani, G. (2001) Blue gourami (Trichogaster trichop terus) model for labyrinth fish. Laser Pages Publishing, Israel, 134.
 Cole, B., Tamura, C.S. and Baile, R. (1999) A manual for commercial production a temporal period spawner. Cen ter of Tropical and Subtropical Aquaculture Publication, 135, 1-32.
 Priest, A. (2002) When two ar three and gold is blue. Osphronemid, 4, 6-11.
 Jackson, K., Abraham, M. and Degani, G. (1994) Oocyte maturation triggered by the presence of male in the blue gourami (Trichogaster trichopterus). Journal of Morphology, 220, 1-9. doi:10.1002/jmor.1052200102
 Degani, G. (1993) The effect of sexual behavior on oocyte development and steroid changes in Trichogaster trichopterus. Copeia, 4, 1091-1196. doi:10.2307/1447089
 Degani, G. (1993) Reproduction control in multi-spawn ing asynchronic Trichogaster trichopterus (Pallas) as a model for the Anabantidae family. Trends in Compara tive Biochemistry and Physiology, 1269-1275.
 Degani, G., Jackson, K. Goldberg, D., Sarfati, R. and Avtalion, R.R. (2003) betaFSH, betaLH and growth hormone gene expression in blue gourami (Trichogaster trichopterus, Pallas 1770) during spermatogenesis and male sexual behavior. Zoological Science, 20, 737-743.
 Degani, G., Yom-Din, S., Goldberg, D. and Jackson, K. (2010) cDNA cloning of blue gourami (Trichogaster trichopterus) prolactin and its expression during the go nadal cycles of males and females. Journal of Endocri nological Investigation, 33, 7-12.
 Goldberg, D., Jackson, K., Yom-Din, S. and Degani, G. (2004) Growth hormone of Trichogaster trichopterus: cDNA cloning, sequencing and analysis of mRNA ex pression during oogenesis. Journal of Aquaculture in the Tropics, 19, 215-229.
 Degani, G. and Boker, R. (1992) Sensitivity to matura tion-inducing steroids and gonadotropin in the oocytes of blue gourami Trichogaster trichopterus (Anabantidae, Pallas, 1770). General and Comparative Endocrinology, 85, 430-439. doi:10.1016/0016-6480(92)90088-2
 Degani, G., Jackson, K., Yom-Din, S. and Goldberg, D. (2006) cDNA cloning and mRNA expression of growth hormone in belontiidae (Anabantoidei suborder) Isr. Journal of Aquaculture, 58, 124-136.
 Levy, G. and Degani, G. (2012) Involvement of GnRH, PACAP and PRP in the reproduction of blue gourami females (Trichogaster trichopterus). Journal of Molecular Neuroscience, 48, 603-616.
 Degani, G., Mananos, E.L., Jackson, K., Abraham, M. and Zohar, Y. (1997) Changes in plasma and pituitary in female blue gourami during the end of vitellogenesis and final oocyte maturation. Journal of Experimental Zoology, 279, 377-385.
 Levy, G..Gothilf, Y., and Degani, G. (2009) Brain go nadotropin releasing hormone3 expression variation dur ing oogenesis and sexual behavior and its effect on pitui tary hormonal expression in the blue gourami. Compara tive Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 154, 241-248.
 Levy, G., Jackson, K. and Degani, G. (2010) Association between pituitary adenylate cyclase-activating polypep tide and reproduction in the blue gourami. General and Comparative Endocrinology, 166, 83-93.
 Levy, G. and Degani, G. (2011) Evidence of a reproduc tion-related function for pituitary adenylate cyclase-acti vating polypeptide-related peptide in an Anabantidae fish. Journal of Molecular Endocrinology, 46, 101-110.
 Jackson, K., Goldberg, D., Ofir, M., Abraham, M. and Degani, G. (1999) Blue gourami (Trichogaster trichop terus) gonadotropic beta subunits (I and II) cDNA se quences and expression during oogenesis. Journal of Mo lecular Endocrinology, 23, 177-187.
 Degani, G., Jackson, K., Goldberg, D., Sarfati, R. and Avtalion, R.R. (2003) betaFSH, betaLH and growth hor mone gene expression in blue gourami (Trichogaster trichopterus, Pallas 1770) during spermatogenesis and male sexual behavior. Zoological Science, 20, 737-743.
 Pfaffl, M.W. (2001) A new mathematical model for rela tive quantification in real-time RT-PCR. Nucleic Acids Research, 29, e45. doi:10.1093/nar/29.9.e45
 Muller, P.Y., Janovjak, H., Miserez, A.R. and Dobbie, Z. (2002) Processing of gene expression data generated by quantitative real-time RT-PCR. Biotechniques, 32, 1372 1379.
 Brauner, C.J. Suvajdzic, K. Nilsson, G. and David, R. (2007) Fish physiology, toxicology, and water quality. Proceedings of the 9th International Symposium, Ecosystems Research Division, Capri.
 Onuma, T.A., Ban, M., Makino, K., Katsumata, H., Hu, W., Ando, H., Fukuwaka, M.A., Azumaya, T. and Urano, A. (2010) Changes in gene expression for GH/PRL/SL family hormones in the pituitaries of homing chum salmon during ocean migration through upstream migration. General and Comperative Endocrinology, 166, 537-548.
 Manzon, L.A. (2002) The role of prolactin in fish osmo regulation: A review. General and Comparative Endo crinology, 125, 291-310. doi:10.1006/gcen.2001.7746
 Hirano, T., Ogasawara, T. Hasegawa, S., Iwata, M. and Nagahama, Y. (1990) Changes in plasma hormone levels during loss of hypoosmoregulatory capacity in mature chum salmon (Oncorhynchus keta) kept in seawater. General and Comperative Endocrinology, 78, 254-262.
 Onuma, T., Kitahashi, T., Taniyama, S., Saito, D., Ando, H. and Urano, A. (2003) Changes in expression of genes encoding gonadotropin subunits and growth hormone/ prolactin/somatolactin family hormones during final ma turation and freshwater adaptation in prespawning chum salmon. Endocrine, 20, 23-34.
 Degani, G. and Gallagher, M.L. (1995) Growth and nutrition of eels. Laser Pages Publishing, Israel, 174
 Nagahama, Y. (1994) Endocrine regulation of gameto genesis in fish. International Journal of Developmental Biology, 38, 217-229.
 Levy, G. and Degani, D. (non-published data) Two GnRH variants in the blue gourami: Gene expression during reproductive cycle and effect on pituitary hormones.
 Weber, G.M., Powell, J.F., Park, M., Fischer, W.H., Craig, A.G., Rivier, J.E., Nanakorn, U., Parhar, I.S., Ngamvongchon, S., Grau, E.G. and Sherwood, N.M. (1997) Evidence that gonadotropin-releasing hormone (GnRH) functions as a prolactin-releasing factor in a teleost fish (Oreochromis mossambicus) and primary structures for three native GnRH molecules. Journal of Endocrinology, 155, 121-132. doi:10.1677/joe.0.1550121
 Ogawa, S., Akiyama, G., Kato, S., Soga, T., Sakuma, Y. and Parhar, I.S. (2006) Immunoneutralization of gonad otropin-releasing hormone type-III suppresses male re productive behavior of cichlids. Neuroscience Letters, 403, 201-205. doi:10.1016/j.neulet.2006.02.041
 Yamamoto, N., Oka, Y. and Kawashima, S. (1997) Lesions of gonadotropin-releasing hor-mone-immunoreactive terminal nerve cells: Effects on the reproductive be havior of male dwarf gouramis. Neuroendocrinology, 65, 403-412. doi:10.1159/000127203
 Mank, J., Promislow, D. and Avise, J. (2005) Phylogenetic perspectives in the evolution of parental care in ray finned fishes. Evolution, 59, 1570-1578.