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 AJPS  Vol.4 No.1 , January 2013
Antibacterial Activity and Phytochemical Analysis of Mentha piperita L. (Peppermint)—An Important Multipurpose Medicinal Plant
Abstract: Mentha piperita L. (Peppermint) is a perennial glabrous and strongly scented herb belonging to family Lamiaceae. The plant is aromatic, stimulant and used for allaying nausea, headache and vomiting. Its oil is one of the most popular widely used essential oils in food products, cosmetics, pharmaceuticals, dental preparations, mouthwashes, soaps and alcoholic liquors. The antibacterial potential of six extracts from leaf, stem and root of Mentha piperita against pathogenic bacteria such as Bacillus subtilis, Streptococcus pneumonia, Staphylococcus aureus, Escherichia coli, Proteus vulgaris and Klebsiella pneumonia were evaluated by agar well diffusion method. The organic (ethanol, methanol, ethyl acetate, chloroform, hexane and petroleum ether) extracts of the leaves were found to possess strong antibacterial activity against a range of pathogenic bacteria. The ethyl acetate leaf extract of Mentha piperita showed pronounced inhibition than chloroform, petroleum ether and hexane. The leaf extract activity being more on Bacillus subtilis, Staphylococcus aureus and Proteus vulgaris than Escherichia coli, Streptococcus pneumonia and Klebsiella pneumonia. In the present study, we also evaluated the phytochemical analysis for the presence of various secondary metabolites. The analysis revealed the presence of alkaloids, flavonoids, steroids, tannins, and phenols.
Cite this paper: P. Sujana, T. Sridhar, P. Josthna and C. Naidu, "Antibacterial Activity and Phytochemical Analysis of Mentha piperita L. (Peppermint)—An Important Multipurpose Medicinal Plant," American Journal of Plant Sciences, Vol. 4 No. 1, 2013, pp. 77-83. doi: 10.4236/ajps.2013.41012.
References

[1]   R. Perumal Samy and S. Ignacimuthu, “Antibacterial Activity of Some Medicinal Plants from Eastern Ghats, South India, Solai Bull,” Ethanopharmacology, Vol. 72, No. 1, 2000, pp. 39 - 41.

[2]   V. Hulin, A. G. Mathot, R. Mafart and L. Dufosse, “Les Proprieties Anti Microbiennes des Huiles Essentielles et Composes Daromes. (Antimicrobial Properties of Essential Oils and Flavour Compounds),” Science Aliments, Vol. 18, No. 6, 1998, pp. 563-582.

[3]   M. A. Ali, M. Saleem, W. Ahmad, M. Parvez and R. Yamdagni, “A Chlorinated Monoterpene Ketone, Acylated-Sitosterol Glycosides and a Flavanone Glycoside from Mentha longifolia (Lamiaceae),” Phytochemistry, Vol. 59, No. 8, 2002, pp. 889-895. doi:10.1016/S0031-9422(01)00490-3

[4]   L. I. Spirling and I. R. Daniels, “Botanical Perspectives on Health Peppermint: More than Just an After-Dinner Mint,” Perspectives in Public Health, Vol. 121, No. 1, 2001, pp. 62-63. doi:10.1177/146642400112100113

[5]   T. Essawi and M. Srour, “Screening of Some Palestian Medicinal Plants for Antibacterial Activity,” Journal of Ethanopharmacology, Vol. 70, No. 3, 2000, pp. 343-349. doi:10.1016/S0378-8741(99)00187-7

[6]   C. Perez, M. Pauli and P. Bazerque, “An Antibiotic Assay by Agar Well Diffusion Method,” Acta Biological Et Medica Experimentalis, Vol. 15, No. 1, 1990, pp. 113-115.

[7]   M. K. Bhargava, H. Singh and A. Kumar, “Evalution of Adhatoda vasica as a Wound Healing Agent in Buffaloes—Clinical Mechanical and Biochemical Studies,” Indian Veterinary Journal, Vol. 65, No. 1, 1988, pp. 33-38.

[8]   S. R. Thaakur, “Antibacterial Activity of Adhatoda vasica Leaf Extract,” Asian Journal of Microbiology, Biotechnology and Environmental Science, Vol. 8, No. 2, 2006, pp. 287 - 289.

[9]   S. J. Pedler and A. W. Berington, “Respiratory Infections,” In: R. Walker and C. Edwards, Eds., Clinical Pharmacy and Therapeutics, Churchill Livingstone Publications, Philadelphia, 2003, pp. 519-529.

[10]   S. K. Singh, K. Saroj, U. J. Tirupathi, A. K. Singh and R. H. Singh, “An Antimicrobial Principle from Sphaeranthus indicus,” International Journal of Crude Drug, Vol. 26, No. 4, 1998, pp. 235-239.

[11]   P. P. Prasad, D. O. Sawaikar, S. R. Rojatkar and B. A. Nagasampagi, “Eudesmanoids from Sphaeranthus indicus,” Fitoterapia, Vol. 71, No. 3, 2000, pp. 264-268. doi:10.1016/S0367-326X(99)00157-4

[12]   M. S. Shekhani, P. M. Shah, A. Yasmin, R. Siddiqui, S. Perveen, K. M. Khan, S. U. Kazmia and Atta-Ur-Rahman, “An Immunostimulant Sesquiterpene Glycoside from Sphaeranthus indicus,” Phytochemistry, Vol. 29, No. 8, 1990, pp. 2573-2576. doi:10.1016/0031-9422(90)85191-H

[13]   T. L. Mason and B. P. Wasserman, “Inactivation of Red Beet Betaglucan Synthase by Native and Oxidized Phenolic Compounds,” Phytochemistry, Vol. 26, No. 8, 1987, pp. 2197-2202. doi:10.1016/S0031-9422(00)84683-X

[14]   A. Scalbert, “Antimicrobial Properties of Tannins,” Phytochemistry, Vol. 30, No. 12, 1991, pp. 3875-3883. doi:10.1016/0031-9422(91)83426-L

[15]   G. A. Jones, T. A. Mc Allister, A. D. Muir and K. J. Cheng, “Effects of Sainfoin (Onobrychis viciifolia scop.) Condensed Tannins on Growth and Proteolysis by Four Strains of Ruminal Bacteria,” Applied Environmental Microbiology, Vol. 60, No. 4, 1994, pp. 1374-1378. PMid:16349244 PMCid:201486

[16]   M. Scortichini and M. Pia Rossi, “Preliminary in Vitro Evaluation of the Antimicrobial Activity of Terpens and Terpenoids towards Erwinia amylovora (Burrill),” Jounal of Applied Bacterialogy, Vol. 71, No. 2, 1991, pp. 109-112. doi:10.1111/j.1365-2672.1991.tb02963.x

[17]   H. Tsuchiya, M. Sato, T. Miyazaki, S. Fujiwara, S. Tanigaki, M. Ohyama, T. Tanaka and M. Linuma, “Comparative Study on the Antibacterial Activity of Phytochemical Flavanones against Methicillin-Resistant Staphylococcus aureus,” Journal of Ethnopharmacology, Vol. 50, No. 1, 1996, pp. 27-34. doi:10.1016/0378-8741(96)85514-0

[18]   M. Serafini, A. Ghiselli and A. Ferro-Luzzi, “Red Wine, Tea and Anti-Oxidants,” Lancet, Vol. 344, No. 8922, 1994, pp. 344-626.

[19]   D. Tejavathi and N. N. Rao, “Comparative Studies between Normal and Tissue Cultured Plants of Bacopa monnieri (L.) Pennel,” In: I. A. Khan and A. khanum, Eds, Role of Biotechnology in Medicinal and Aromatic Plants, Ukaaz Publications, Hyderabad, 1998, pp. 239-247.

 
 
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