AS  Vol.4 No.1 , January 2013
Peculiarities of feed contamination with citrinin and ochratoxin A
Occurrence of citrinin and ochratoxin A in different feed ingredients and compound feeds was screened by accredited methods based on the indirect competitive enzyme-linked immunosorbent assay. High frequency co-occurrence of both toxins was found in wheat grain and processed sunflower seeds. Citrinin levels exceeded those of ochratoxin A in the majority of co-contaminated feed samples, and the ratio of (1.1 - 10):1 proved to be the most frequent. A possible role of Aspergillus and Penicillium fungi in separate and simultaneous OTA and CIT occurrence in feeds is also discussed.

Cite this paper
Kononenko, G. and Burkin, A. (2013) Peculiarities of feed contamination with citrinin and ochratoxin A. Agricultural Sciences, 4, 34-38. doi: 10.4236/as.2013.41006.
[1]   Krough, P. and Hasselager, E. (1968) Studies on fungal nephtotoxicity. In: Royal Veterinary and Agricultural College Year-book, Copenhagen, 198-214.

[2]   Krough, P. (1972) Natural occurrence of ochratoxin A and citrinin in cereals associated with field outbreaks of swine nephropathy. Proceedings of IUPAC-Sponsored Symposium: Control of Mycotoxins, Go-tenburg, 19.

[3]   Krough, P., Hald, B. and Pedersen, E.J. (1973) Occurrence of ochratoxin A and citrinin in cereals associated with mycotoxic porcine nephropathy. Acta Pathologica et Microbiologica Scandinavica Section B, 81, 689-695.

[4]   Scott, P.M., Walbeek, W., van Harwig, J. and Fennell, D.I. (1970) Occurrence of a mycotoxin, ochratoxin A, in wheat and isolation of ochratoxin A and citrinin producing strains of Penicillium viridicatum. Canadian Journal of Plant Science, 50, 583-585. doi:10.4141/cjps70-108

[5]   Scott, P.M., Walbeek, W., van Kennedy, B. and Anyeti, D. (1972) Mycotoxins (ochratoxin A, citrinin, and sterigmatocystin) and toxigenic fungi in grains and other agricultural products. Agricultural and Food Chemistry, 20, 1103-1109. doi:10.1021/jf60184a010

[6]   Prior, M.G. (1976) Mycotoxin determination on animal feedstuffs and tissues in Western Canada. Canadian Journal of Comparative Medicine, 40, 75-79.

[7]   Ahamad, D.B. and Vairamuthu, S. (2000) Occurrence of citrinin mycotoxin in compounded feeds and various feed ingredients of poultry ration in Namakkal area (Tamil Nadu). Indian Journal of Environmental Toxicology, 10, 84-86.

[8]   Pfohl-Leszkowicz, A. and Manderville, R.A. (2007) Ochratoxin A: An overview on toxicity and carcinogenicity in animals and humans. Molecular Nutrition & Food Research, 51, 61-99. doi:10.1002/mnfr.200600137

[9]   Kononenko, G.P. and Burkin, A.A. (2008) A survey on the occurrence of citrinin in feeds and their ingredients in Russia. Mycotoxin Research, 24, 3-6. doi:10.1007/BF02985263

[10]   Kononenko, G.P. and Soboleva, N.A. (2004) Improvement of methods for ochratoxin A determination in feeds based on enzyme immunoassay. Russian Agricultural Sciences, 6, 23-27.

[11]   Burkin, A.A., Kononenko, G.P. and Soboleva, N.A. (2005) Ochratoxin A contamination of grain feeds. Russian Agricultural Sciences, 3, 32-35.

[12]   Suganthi, M.P.F., Ravi, R., Purushothaman, M.R., Monah, B. and Vasanthakumar, P. (2003) Occurrence of ochratoxin A in sunflower cake in poultry. Indian Journal of Poultry Science, 38, 37-41.

[13]   Hanif, N.Q., Muhammad, N., Khatoon, S. and Malik, N. (2005) Prevalence of mycotoxins in poultry finished feed. Journal of Animal and Veterinary Advances, 4, 189-193.

[14]   Meister, U. (2003) Detection of citrinin in ochratoxin Acontaining products by a new HPLC method. Myco-toxin Research, 19, 27-30. doi:10.1007/BF02940087

[15]   Stahr, H.M., Domoto, M., Zhu, B.L. and Pfeiffer, R. (1985) Chemical analysis for ochratoxin poisoning. Mycotoxin Research, 1, 31-35. doi:10.1007/BF03191952

[16]   Vrabcheva, T., Usleber, E., Dietrich, R. and Martlbauer, E. (2000) Co-occurrence of ochratoxin A and citrinin in cereals from Bulgarian villages with a history of Balkan endemic nephropathy. Journal of Agricultural and Food Chemistry, 48, 2483-2488. doi:10.1021/jf990891y

[17]   Frank, H.K. (1992) Citrinin. Zeit-schrift fur Ernahrung-swissenschaft, 31, 164-177. doi:10.1007/BF01611139

[18]   Varga, J., Rigo, K., Teren, J. and Mesterhazy, A. (2001) Recent advances in ochratoxin research I. Production, detection and occurrence of ochratoxins. Cereal Research Communications, 29, 85-92.

[19]   Eroshkin, A.A., Soboleva, N.A., Burkin, A.A. and Kononenko, G.P. (2000) Ochratoxin A producers in grain feeds. Problemy veterinarnoi sanitarii i ekologii. Sbornik nauchnych trudov, 109, 134-144.

[20]   Vasyl’ev, D.A., Piryazeva, E.A. and Soboleva, N.A. (2009) A high active producer of ochratoxin A from forage grain. Veterinarnaya Patologiya, 30, 72-75.

[21]   Bayman, P., Baker, J.L., Doster, M.A., Michailides, T.J. and Mahoney, N.E. (2002) Ochratoxin production by the Aspergillus ochraceus Group and Aspergillus alliaceus. Applied and Environ-mental Microbiology, 68, 2326-2329. doi:10.1128/AEM.68.5.2326-2329.2002

[22]   Czerwiecki, L., Czajkowska, D. and Witkowska-Gwiazdowska, A. (2002) On ochratoxin A and fungal flora in polish cereals from conventional and ecological farms. Part I. Occurrence of ochratoxin A and fungi in cereals in 1997. Food Additives and Contaminants, 19, 470-477. doi:10.1080/02652030110113726

[23]   Boley, A. and Muller, H.M. (1986) Production of ochratoxin A, citrinin and ergosterol by Penicillium viridicatum in autoclaved and non-autoclaved wheat at low temperature. Mycotoxin Research, 2, 99-103. doi:10.1007/BF03191970

[24]   Harwig, J. and Chen, Y.K. (1974) Some conditions favoring production of ochratoxin A and citrinin by Penicillium viridicatum in wheat and barley. Canadian Journal of Plant Science, 54, 17-22. doi:10.4141/cjps74-003

[25]   Krough, P. (1973) Nephropathy caused by mycotoxins from Penicillium and Aspergillus. Journal of Genetics and Microbiology, 73, 34-35.

[26]   Krough, P., Hasselager, E. and Friis, P. (1970) Studies on fungal nephro-toxicity. II. Isolation of 2 nephrotoxic compounds from Penicillium viridicatum West: Citrinin and oxalic acid. Acta Patho-logica et Microbiologica Scandinavica Section B, 78, 401-413.

[27]   Pitt, J.I. (1987) Penicillium viridicatum, Penicillium verrucosum, and production of ochratoxin A. Applied and Environmental Microbiology, 53, 266-269.

[28]   Scudamore, K.A., Clarke, J.H. and Hetmanski, M.T. (1993) Isolation of Penicillium strains producing ochratoxin A, citrinin, xantho-megnin, viomellein and vioxanthin from stored cereal grains. Letters in Applied Microbiology, 17, 82-87. doi:10.1111/j.1472-765X.1993.tb00377.x