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 FNS  Vol.4 No.1 , January 2013
Assessment of Microbial Changes and Nutritional Qualities of Extruded White Yam (Dioscorea rotundata) and Bambara Groundnut (Vigna subterranean) Blends
Abstract: Two flavoured extruded products were developed by co-extruding yam grits (750 μm) obtained from white yam (Dioscorea rotundata) and bambara groundnut flour (250 μm) in 160:40 respectively obtained from white bambara groundnut with added flavouring agents of salt (1% - 3%) and sugar (4% - 6%) in the feed blends at screw speed of 70 rpm, 17.5%, feed moisture and at the barrel temperature of 145℃. The extruded products were packaged in low density polyethylene bag (0.02 μm gauge size) and stored at room temperature (28℃ ± 2℃) and at refrigeration temperature (9℃ ± 2℃) for a period of twenty weeks. The microbiological changes in the extruded products as determined by the total plate under both storage conditions showed that maximum total plate counts were 0.5 × 104 and 5.4 × 104 cfu/g at 9℃ ± 2℃ and 28℃ ± 2℃ respectively. Nutritional evaluation studies of extrudates were comparable (p ≥ 0.05) with standard casein diet with minimum crude protein content of 13.51% providing 1707.2 KJ energy per 100gof diet and supported weight gain and growth of laboratory animals.
Cite this paper: O. Oluwole, S. Awonorin, F. Henshaw, G. Elemo and O. Ebuehi, "Assessment of Microbial Changes and Nutritional Qualities of Extruded White Yam (Dioscorea rotundata) and Bambara Groundnut (Vigna subterranean) Blends," Food and Nutrition Sciences, Vol. 4 No. 1, 2013, pp. 100-107. doi: 10.4236/fns.2013.41014.
References

[1]   P. Colona and C. Mercier, “Macromolecular Modifications of Manioc Starch Components by Extrusion Cooking with and without Lipids,” Carbohydrate Polymer, Vol. 3, No. 2, 1983, pp. 87-128. doi:10.1016/0144-8617(83)90001-2

[2]   F. Hsieh, I. C. Peng and H. E. Huff, “Effect of Salt, Sugar and Screw Speed on Processing and Product Variable of Cornmeal Extruded with a Twin Screw Extruder,” Journal of Food Science, Vol. 55, No. 1, 2006, pp. 224-227. doi:10.1111/j.1365-2621.1990.tb06057.x

[3]   I. Nkama and K. B. Filli “Development and Characteristics of Extruded Fura from Mixtures of Pearl Millet and Grains Legume Flours,” International Journal of Food Properties, Vol. 9, No. 2, pp. 157-165. doi:10.1080/10942910600596605

[4]   K. B. Filli, I. Nkama, V. A. Jideani and M. U. Abubakar, “Influence of Extrusion Variables on Some Functional Properties of Extruded Millet-Soybean for the Manufacture of ‘Fura’,” African Journal of Food Science, Vol. 46, No. 6, 2010, pp. 342-352.

[5]   R. Chinnaswamy and M. A. Hanna, “Optimum Extrusion Cooking Conditions for Maximum Expansion of Corn Starch,” Journal of Food Science, Vol. 53, No. 3, 1988, pp. 834-836. doi:10.1111/j.1365-2621.1988.tb08965.x

[6]   A. Olapade and D. O. Adetuyi, “Comparison of Different Methods of Producing Bambara (Voandzeia subterranean L. Thou) Flours for Preparation of ‘Moin-Moin’,” Nigerian Food Journal, Vol. 25, No. 2, 2007, pp. 150-157.

[7]   G. Osuji, “Advances in Yam Research. The Biochemistry and Technology of the Yam Tuber,” University Press Nigeria, Anambra State, 1990, p. 50.

[8]   S. E. Omonigho and M. J. Ikenebomeh, “Effect of Temperature Treatment on the Chemical Composition of Pounded White Yam during Storage,” Food Chemistry, Vol. 71, No. 2, 2000, pp. 215-220. doi:10.1016/S0308-8146(00)00158-8

[9]   T. S. Barimala, S. C. Achineuhu, L. Yibatama and E. N. Amadi, “Studies on the Solid Substrate Fermentation of Bamabara Groundnut,” Journal of Science Food and Agriculture, Vol. 66, No. 4, 1994, pp. 443-446. doi:10.1002/jsfa.2740660404

[10]   A. O. Odior1 and E. S. Orsarh, “Design and Construction of a Yam Pounding Machine,” International Journal of Natural and Applied Sciences, Vol. 4, No. 3, 2008, pp. 319-323.

[11]   W. F. Harrigan and M. E. Mclance, “Laboratory Methods in Microbiology,” Academic Press, New York, 1976, pp. 8-40, 106-112, 128, 199-204.

[12]   AOAC, “Official Methods of Association of Analytical Chemist,” 18th Edition, Washington DC, 2005.

[13]   H. Egan, R. S. Kirk and R. Sawyer, “Pearson’s Chemical Analysis of Foods,” 8th Edition, Churchhill Living Stone, London, 1991.

[14]   C. S. James, “Analytical Chemistry of Food,” Aspen Publishers Incorporated, Gaithersburg, 1998, pp. 64-65, 71-135.

[15]   D. Pearson, “The Chemical Analysis of Foods,” 7th Edition, Churchill Living Stone, London, 1976, pp. 19, 23-24.

[16]   M. L. Kakade, J. J. Rackis, J. E. Mcghee and G. Puski, “Determination of Trypsin Inhibitor Activity of Soy Products. A Collaborative Analysis of an Improved Procedure,” Journal of Cereal Chemistry, Vol. 51, 1974, pp. 376-382.

[17]   M. I. Lape, “Nutritional Quality of Yam (Dioscorea dumetorwin and D. rotundata) Flour for Growing Rats,” Journal of Science of Food and Agriculture, Vol. 66, No. 4, 1994, pp. 447-455. doi:10.1002/jsfa.2740660405

[18]   A. O. Fasuyi, “Nutritional Evaluation of Cassava (Manihot esculenta Crantz) leaf Protein Concentrate (CLPC): An Alternative Protein Sources in Rat Assay,” Pakistan Journal of Nutrition, Vol. 4, No. 1, 2005, pp. 50-56. doi:10.3923/pjn.2005.50.56

[19]   I. C. Obizoba, “The Nutritive Value of Mixtures of Two Varieties of Bambara Groundnut and Sorghum,” Journal of Cereal chemistry, Vol. 66, No. 4, 1989, pp. 249-259.

[20]   M. Zimmermon, “Ethical Guideline for Investigation of Experimental Pain in Conscious Animal,” Pain, Vol. 16, No. 2, 1983, pp. 109-110. doi:10.1016/0304-3959(83)90201-4

[21]   O. A. T. Ebuehi and O. O. Aleshinloye, “Extracts of Cnestis ferruginea and Rawolfia vomitoria Affect Blood Chemistry and GABAergic Neurotransmission in Ketamine—Induced Psychotic Rats,” Nigerian Quarterly Journal of Hospital Medicine, Vol. 22, No. 4, 2010, pp. 171-176.

[22]   PHLSG, “The Microbiological Quality of Ready-to-Eat Foods Sampled at the Point of Sale, (Public Health Laboratory Service Guidelines),” Brough Council, 2008.

[23]   N. W. Destrosier and J. N. Destrosier, “The Technology of Food Preservation,” 4th Edition, CBS Publisher and Distributors, New Delhi, 2004, pp. 340-363.

[24]   C. W. Bamforth, “Food, Fermentation and Micro-Organisms,” Blackwell Publishing Company, Oxford, 2005, pp. 17-18. doi:10.1002/9780470995273

[25]   C. H. Collins, P. M. Lyne and J. M. Grange, “Microbiological Methods,” 6th Edition, Buttermorths, London, 1989, pp. 178, 198.

[26]   S. H. Brough and S. N. Azam-Alli, “The Effect of Soil Moisture on the Proximate Composition of Bambara Groundnut,” Journal of Science Food and Agriculture, Vol. 60, No. 2, 1992, pp. 177-203. doi:10.1002/jsfa.2740600207

[27]   P. McDonald, R. A. Edward and J. F. D. Greenhalgh, “Animal Nutrition,” 3rd Edition, Oliver and Boyd, 1979.

[28]   T. U. Nwabueze, “Growth Performance of Rats Fed Raw and Extruded African Breadfruit Based Complementary Diet. A Response Surface Analysis,” Journal of Science of Food and Agriculture, Vol. 88, No. 3, 2008, pp. 522-528. doi:10.1002/jsfa.3116

[29]   V. Baskran and A. Bhattacharaya, “Nutritional Statics of the Protein of Corn-Soya Based Extruded Products Evaluated by Rat Bioassay,” Plant Foods for Human Nutrition, Vol. 59, No. 3, 2005, pp. 101-104. doi:10.1007/s11130-004-4309-3

[30]   T. U. Nwabueze, “Bioavailability of Vitamins and Minerals in Adult Rats Fed Raw and Extruded African Bread Fruit (Treculia africana) Mixtures,” Journal of Food Agriculture and Environment, Vol. 5, No. 314, 2007, pp. 465-470.

[31]   T. U. Nwabueze, “Weight Analysis and Nitrogen Balance Assay in Rat Fed Extruded African Bread Fruit Based Diets,” Nigerian Food Journal, Vol. 46, No. 1, 2008, pp. 27-41.

[32]   K. Owusu-Domfen, “Trypsin Inhibitor Activity of Cowpea (Vigna unguiculata) and Bambara Beans (Voandzera subteranea),” Ghana Journal of Science, Vol. 5, 1972, pp. 99-102.

[33]   G. R. Jansen, J. M. D Harper and L. Dean, “Nutritional Evaluation of Blended Foods Made with a low Cost Extruder Cooker,” Journal of Food Science, Vol. 44, No. 3, 1978, pp. 1322-1328.

 
 
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