The effect of cadmium chloride (150mg/l) and mercury (II) chloride (80mg/l) either alone or in combination in drinking water for 4 weeks on function and structure of the liver of male rats was studied. Results indicated that the ratio of liver weight to body weight and the activities of serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase noted in rats co-exposed to cadmium and mercury were intermediate between those noted in the individually metal treated rats. The histopathological study showed that the individual metal and the combined metal treatments caused severe liver damage. The degree of these changes noted in rats co-exposed to cadmium and mercury was not higher than those signalized in individual treatment. The biochemical and the histological changes observed in rats co-exposed to cadmium and mercury show that there is not an additive effect between these two metals.
Cite this paper
S. Haouem, I. Chargui, M. Najjar, B. Sriha and A. El Hani, "Liver Function and Structure in Rats Treated Simultaneously with Cadmium and Mercury," Open Journal of Pathology, Vol. 3 No. 1, 2013, pp. 26-31. doi: 10.4236/ojpathology.2013.31005.
 S. Satarug, J. R. Baker, S. Urbenjapol, M. R. Haswell-Elkins, P. E. B. Reilly and D. J. Williams, “A Global Perspective on Cadmium Pollution and Toxicity in Non-Occupationally Exposed Population,” Toxicology Letters, Vol. 137, No. 1-2, 2003, pp. 65-83.
 H. W. Schenkel, “Nutritive Beeinflssung des Stoffwechsel-Verhalten von Cadmium bei Rind und Schwein,” Habilitationsschrift Hohencheim, 1988.
 G. C. Cotzias, D. C. Borg and B. Selleck, “Virtual Absence of Turnover in Cadmium Metabolism: Cd109 Studies in the Mouse,” American Journal of Physiology, Vol. 201, No. 5, 1961, pp. 927-930.
 G. F. Nordberg and T. Kjellstrom, “Metabolic Model for Cadmium in Man,” Environmental Health Perspectives, Vol. 28, 1979, pp. 211-217. doi:10.1289/ehp.7928211
 D. F. Hwang and L. C. Wang, “Effect of Taurine on Toxicity of Cadmium in Rats,” Toxicology, Vol. 167, No. 3, 2001, pp. 173-180. doi:10.1016/S0300-483X(01)00472-3
 P. L. Goering, D. L. Morgan and S. F. Ali, “Effect of Mercury Vapour Inhalation on Reactive Oxygen Species and Antioxidant Enzymes in Rat Brain and Kidney Are Minimal,” Journal of Applied Toxicology, Vol. 22, No. 3, 2002, pp.167-172. doi:10.1002/jat.844
 S. Ekino, M. Susa, T. Ninomiya, K. Imamura and T. Kitamura, “Minamata Disease Revisited: An Update on the Acute and Chronic Manifestations of Methyl Mercury Poisoning,” Journal of the Neurological Sciences, Vol. 262, No. 1-2, 2007, pp. 131-144.
 S. Yannai and K. M. Sachs, “Absorption and Accumulation of Cadmium, Lead and Mercury from Foods by Rats,” Food and Chemical Toxicology, Vol. 31, No. 5, 1993, pp. 351-355. doi:10.1016/0278-6915(93)90190-A
 I. Bando, M. I. Reus, D. Andrés and M. Cascales, “Endogenous Antioxidant Defense System in Rat Liver Following Mercury Chloride Oral Intoxication,” Journal of Biochemical and Molecular Toxicology, Vol. 19, No. 3, 2005, pp. 154-161. doi:10.1002/jbt.20067
 A. Koyu, A. Gokcimen, F. Ozguner, D. S. Bayram and A. Kocak, “Evaluation of the Effects of Cadmium on Rat Liver,” Molecular and Cellular Biochemistry, Vol. 284, No. 1-2, 2006, pp. 81-85.
 R. Agarwal, S. K. Goel, R. Chandra and J. R. Behari, “Role of Vitamin E in Preventing Acute Mercury Toxicity in Rat,” Environmental and Toxicology and Pharmacology, Vol. 29, No. 1, 2010, pp. 70-78.
 L. D. Koller, B. V. Stang and M. V. De l. R. Mendez, “Pathology of Toxic Oils and Selected Metals in the MRL/lpr Mousse,” Toxicology Letters, Vol. 29, No. 6, 2001, pp. 630-638.
 S. A. El-Maraghy, M. Z. Gad, A. T. Fahim and M. A. Hamdy, “Effect of Cadmium and Aluminum Intakeon the Antioxidant Status and Lipid Peroxidation in Rat Tissues,” Journal of Biochemical and Molecular Toxicology, Vol. 15, No. 4, 2001, pp. 207-214. doi:10.1002/jbt.18
 M. Kumar, M. K. Sharma and A. Kumar, “A Food Supplement against Mercury Induced Hepatic Toxicity,” Journal of Health Science, Vol. 51, No. 4, 2005, pp. 424-430. doi:10.1248/jhs.51.424
 M. M. Brzoska, J. Moniuszko-Jakoniuk, B. Pilat-Marcinkiewicz and B. Sawickl, “Liver and Kidney Function and Histology in Rats Exposed to Cadmium and Ethanol,” Alcohol and Alcoholism, Vol. 38, No. 1, 2003, pp. 2-10. doi:10.1093/alcalc/agg006
 L. Pari and P. Murugavel, “Role of Diallyl Tetrasulfide in Ameliorating the Cadmium Induced Biochemical Changes in Rats,” Environmental and Toxicology and Pharmacology, Vol. 20, No. 3, 2005, pp. 493-500.
 M. K. Sharma, R. Patni, M. Kumar and A. Kumar, “Modification of Mercury-Induced Biochemical Alterations in Blood of Swiss Albino Mice by Spirulina fusiformis,” Environmental and Toxicology and Pharmacology, Vol. 20, No. 2, 2005, pp. 289-296.
 A. Sundberg, E. L. Appelkwist, G. Dallner and R. Nilsson, “Glutathione Transferase in the Urine: Sensitive Methods for Detection of Kidney Damage Induced by Nephrotoxic Agents in Humans,” Environmental Health Perspectives, Vol. 102, Suppl. 3, 1994, pp. 293-296.
 E. H. Jihen, M. Imed, H. Fatima and K. Abdelhamid, “Protective Effects of Selenium (Se) and Zinc (Zn) on Cadmium (Cd) Toxicity in the Liver and Kidney of the Rat: Histology and Cd Accumulation,” Food and Chemical Toxicology, Vol. 46, No. 11, 2008, pp. 3522-3527.
 J. B. T. Rocha, M. E. Pereira, T. Emanuelli, R. S. Christofari and D. O. Souza, “Effect of Treatment with Mercuric Chloride and Lead Acetate during the Second Stage of Rapid Post-Natal Brain Growth on ALA-D Activity in Brain, Liver and Blood of Suckling Rats,” Toxicology, Vol. 100, No. 1-3, 1995, pp. 27-37.
 M. Farina, R. Brandao, F. S. Lara, F. A. A. Soares, D. O. Souza and J. B. T. Rocha, “Mechanisms of the Inhibitory Effects of Selenium and Mercury on the Activity of d-Aminolevulinate Dehydratase from Mouse Liver, Kidney and Brain,” Toxicology Letters, Vol. 139, No. 1, 2003, pp. 55-66. doi:10.1016/S0378-4274(02)00454-X
 E. K. Jaffe, “Porphobilinogen Synthase, the First Source of Heme’s Asymmetry,” Journal of Bioenergetics and Biomembranes, Vol. 27, No. 2, 1995, pp. 169-179.
 E. N. Maciel, R. C. Bolzan, A. L. Braga and J. B. T. Rocha, “Diphenyl Diselenide and Diphenyl Ditelluride Differentially Affect Delta-Aminolevulinate Dehydratase from Liver, Kidney, and Brain of Mice,” Journal of Biochemical and Molecular Toxicology, Vol. 14, No. 6, 2000, pp. 310-319.
 R. Neal, P. Yang, J. Fiechtl, D. Yildiz, H. Gurer and N. Ercal, “Pro-Oxidant Effects of Delta-Aminolevulinic Acid (Delta-ALA) on Chinese Hamster Ovary (CHO) Cells,” Toxicology Letters, Vol. 91, No. 3, 1997, pp. 169-178.
 F. G. Princ, A. A. Juknat, A. A. Amitrano and A. Batlle, “Effect of Reactive Oxygen Species Promoted by Deltaaminolevulinic Acid on Porphyrin Biosynthesis and Glucose Uptake in Rat Cerebellum,” General Pharmacology, Vol. 31, No. 1, 1998, pp. 143-148.
 M. Yusof, D. Yildiz and N. Ercal, “N-Acetyl-L-Cysteine Protects against Delta-Aminolevulinic Acid-Induced 8-Hydroxydeoxyguanosine Formation,” Toxicology Letters, Vol. 106, No. 1, 1999, pp. 41-47.
 C. W. Nogueira, F. A. Soares, P. C. Nascimento, D. Muller and J. B. T. Rocha, “2,3-Dimercaptopropane-1-Sulfonic Acid and Meso-2,3-Dimercaptosuccinic Acid Increase Mercury and Cadmium Induced Inhibition of d-Aminole-vulinate Dehydratase,” Toxicology, Vol. 184, No. 2-3, 2003, pp. 85-95. doi:10.1016/S0300-483X(02)00575-9