ABB  Vol.4 No.1 , January 2013
Development and characterization of polymeric nanoparticles as Barbatimão (Stryphnodendron obovatum) standardized fraction carrier
Abstract: Recent studies have shown that Barbatimao fractions are promising for dermatological diseases treatment, due to tannins content and its strong antioxidant activity. In this study, chitosan was used as polymeric matrix for the development of nanoparticulated system able to deliver a Barbatimao standardized fraction. Nanoparticles were prepared by ionotropic gelation method involving the mixing of two aqueous phases at room temperature. The product was characterized by infrared absorption spectrometry, scanning electron microscopy, quantification of fraction in the formulation, in vitro release profile, size of particles and polydispersity index. The level of Barbatimao fraction obtained in the formulation was 38.23%, with 30% of encapsulated fraction released in 7 days. No chemical reaction between the fraction and the matrix could be noticed. The particles containing fraction presented an average size of 241 nm and polydispersity index of 0.484. It seems that the phytotherapic formulation could be used as drug delivery system for sustained release of Barbatimao fraction.
Cite this paper: Costa, D. , Franca, J. , Ribeiro, T. , Kaplan, M. , Faraco, A. and Castilho, R. (2013) Development and characterization of polymeric nanoparticles as Barbatimão (Stryphnodendron obovatum) standardized fraction carrier. Advances in Bioscience and Biotechnology, 4, 89-92. doi: 10.4236/abb.2013.41013.

[1]   Davies, K.J. (1995) Oxidative stress: The paradox of aerobic life. Biochemical Society Symposia, 61, 1-31.

[2]   Halliwell, B. (1999) Antioxidant defense mechanisms: From the beginning to the end (of the beginning). Free Radical Research, 31, 261-272. doi:10.1080/10715769900300841

[3]   Favier, A.E., Cadet, J., Kalyanaraman, B., Fontecave, M. and Pierre, J.L. (1995) Analysis of free radicals in biological systems. 1st Edition, Birkhauser Verlag, Basel. doi:10.1007/978-3-0348-9074-8

[4]   Ramalho, V.C. and Jorge, N. (2006) Antioxidantes utilizados em óleos, gorduras e alimentos gordurosos. Química Nova, 29, 755-760. doi:10.1590/S0100-40422006000400023

[5]   Nascimento, A.M. (2008) Avaliacao da qualidade de extratos de Stryphnodendron adstringens (Martius) Coville. Master Dissertation, Federal University of Minas Gerais, Belo Horizonte.

[6]   Fuente, M., et al. (2010) Chitosan-based nanostructures: A delivery platform for ocular therapeutics. Advanced Drug Delivery Reviews, 62, 100-117. doi:10.1016/j.addr.2009.11.026

[7]   Rodrigues, L.B., et al. (2009) In vitro release and characterization of chitosan films as dexamethasone carrier. International Journal of Pharmaceutics, 368, 1-6. doi:10.1016/j.ijpharm.2008.09.047

[8]   Keawchaoon, L. and Yoksan, R. (2011) Preparation, characterization and in vitro release study of carvacrol-loaded chitosan nanoparticles. Colloids and Surfaces B, 84, 163-171. doi:10.1016/j.colsurfb.2010.12.031

[9]   Nagpal, K., Singh, S.K. and Mishra, D.N. (2010) Chitosan nanoparticles: A promising system in novel drug delivery. Chemical Pharmaceutical Bulletin., 58, 1423-1430. doi:10.1248/cpb.58.1423

[10]   Calvo, P., Remu ñán-López, C., Vila-Jato, J.L. and Alonso M.J. (1997) Novel hydrophilic chitosan-polyethylene oxide nanoparticles as protein carriers. Journal of Applied Polymer Science, 63, 125-132. doi:10.1002/(SICI)1097-4628(19970103)63:1<125::AID-APP13>3.0.CO;2-4

[11]   Nuntanakorn, P., et al. (2007) Analysis of polyphenolic compounds and radical scavenging activity of four American Actaea species. Phytochemical Analysis, 18, 219-228. doi:10.1002/pca.975

[12]   Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995) Use of a free radical method to evaluate antioxidant activity. Lebensmittel Wissenschaft & Technologie, 28, 25-30. doi:10.1016/S0023-6438(95)80008-5

[13]   Zhang, Y., Yang, Y., Tang, K., Hu, X. and Zou, G. (2008) Physicochemical characterization and antioxidant activity of quercetin-loaded chitosan nanoparticles. Journal of Applied Polymer Science, 107, 891-897. doi:10.1002/app.26402

[14]   Jin, Z.Q. and Chen, X. (1998) A simple reproducible model of free radical-injured isolated heart induced by 1,1-diphenyl-2-picryl-hydrazyl (DPPH). Journal of Pharmacological and Toxicological Methods, 39, 63-70. doi:10.1016/S1056-8719(97)00093-2

[15]   Costa, D.F.G., Franca, J.R., Faraco, A.A.G and Castilho, R.O. (2010) Método de atividade antioxidante (DPPH) para a quantificacao do extrato padronizado de Barbatimao em formulacao farmacêutica polimérica nanoparticulada. Proceedings of the 21th Simpósio de Plantas Medicinais do Brasil, João Pessoa, 14-17 October 2010.