Li Fang, Wenqing Liu, Nanjing Zhao^*, Jingbo Duan, Zhigang Wang, Yujun Zhang, Xue Xiao, Jing Liu, Gaofang Yin, Chaoyi Shi

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Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, 350 Shu Shan Hu Road, Hefei, Anhui 230031, China.
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Abstract: Different temperatures of water bath was used to extract the intracellular microcystin-LR(MC-LR) of Microcystis aeruginosa. Researching the extraction efficiency under the suitable temperature, so that it could find out the best temperature and time for extracting MC-LR from Microcystis aeruginosa cells. Five equal Microcystis aeruginosa was used to find out the best temperature, extracting at 60℃, 70℃, 80℃, 90℃ and 100℃ for 15 minutes, respectively. Results showed that the content of MC-LR extracted with the water under 100℃ was the highest. But meanwhile, the type and the content of impurities was the highest, too. In addition, another six equal Microcystis aeruginosa was extract with the water under 100℃ for 5min, 10 min, 15 min, 20 min, 25 min and 30 min respectively. It was proved that 20 minutes was enough for extracting MC-LR from Microcystis aeruginosa, no long time was needed.

Keywords: Boiling Water Bath, Extract, Microcystin-LR

Cite this paper: L. Fang, W. Liu, N. Zhao, J. Duan, Z. Wang, Y. Zhang, X. Xiao, J. Liu, G. Yin and C. Shi, "Studies on Extracting Microcystin-Lr from Microcystis Aeruginosa by Water Bath," Journal of Environmental Protection, Vol. 4 No. 1, 2013, pp. 70-73. doi: 10.4236/jep.2013.41B013.

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