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 JEP  Vol.4 No.1 B , January 2013
Studies on Extracting Microcystin-Lr from Microcystis Aeruginosa by Water Bath
Abstract: Different temperatures of water bath was used to extract the intracellular microcystin-LR(MC-LR) of Microcystis aeruginosa. Researching the extraction efficiency under the suitable temperature, so that it could find out the best temperature and time for extracting MC-LR from Microcystis aeruginosa cells. Five equal Microcystis aeruginosa was used to find out the best temperature, extracting at 60℃, 70℃, 80℃, 90℃ and 100℃ for 15 minutes, respectively. Results showed that the content of MC-LR extracted with the water under 100℃ was the highest. But meanwhile, the type and the content of impurities was the highest, too. In addition, another six equal Microcystis aeruginosa was extract with the water under 100℃ for 5min, 10 min, 15 min, 20 min, 25 min and 30 min respectively. It was proved that 20 minutes was enough for extracting MC-LR from Microcystis aeruginosa, no long time was needed.
Cite this paper: L. Fang, W. Liu, N. Zhao, J. Duan, Z. Wang, Y. Zhang, X. Xiao, J. Liu, G. Yin and C. Shi, "Studies on Extracting Microcystin-Lr from Microcystis Aeruginosa by Water Bath," Journal of Environmental Protection, Vol. 4 No. 1, 2013, pp. 70-73. doi: 10.4236/jep.2013.41B013.
References

[1]   ZHANG Ming-ming,PAN Gang,YAN Hai et al.A method to extract algae toxin of microcystin-LR[J].Journal of Environmental Sciences. 16(4):694-696.(2004)

[2]   REN Jing, WANG Xiang-rong, HAO Meng-li. Optimization of extracting microcystins in the cells of Microcystis aeruginosa with HPLC. Sciencepaper Online.

[3]   Jarkko Rapala, Kirsti Erkomaa, Jaana Kukkonen et al. Detection of microcystins with protein phosphatase inhibition assay, high-performance liquid chromatography-UV detection and enzyme-linked immunosorbent assay Comparison of methods. Analytica Chimica Acta. 466:213-231.(2002)

[4]   TANG Hong-feng, SUN Qian, MU Ting et al. The comparison of extracting microcystins in natural water bloom cyanobacteria[J]. Chemistry & Bioengineering. 28(1):82-84.(2011)

[5]   James S.Metcalf,Geoffrey A.Codd.Microwave oven and boiling waterbath extraction of hepatotoxins from cyanobacterial cells.FEMS Microbiology Letters.184:241-246.(2000)

[6]   LI Qiu-xia, CAI Chao-hai, XU Gui-lan. Rapid detection of microcystins-LR in drinking water with super fast performance liquid chromatography-tandem mass spectrometry. Chin J Health Lab Technol. 20(8):1875-1877.(2010)

[7]   LEI La-mei, Gan Nan-qin, SONG Li-rong. A rapid method to extract and analyse microcystins. ACTA Hydrobiologica Sinica. 27(5):468-471.(2003)

[8]   National Standards of Peoples Republic of China. GB/T 20466-2006. The detection of microcystins in water.

 
 
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