Studies on Extracting Microcystin-Lr from Microcystis Aeruginosa by Water Bath
Author(s)
Li Fang,
Wenqing Liu,
Nanjing Zhao*,
Jingbo Duan,
Zhigang Wang,
Yujun Zhang,
Xue Xiao,
Jing Liu,
Gaofang Yin,
Chaoyi Shi
ABSTRACT
Different temperatures of water bath was used to extract the intracellular microcystin-LR(MC-LR) of Microcystis aeruginosa. Researching the extraction efficiency under the suitable temperature, so that it could find out the best temperature and time for extracting MC-LR from Microcystis aeruginosa cells. Five equal Microcystis aeruginosa was used to find out the best temperature, extracting at 60℃, 70℃, 80℃, 90℃ and 100℃ for 15 minutes, respectively. Results showed that the content of MC-LR extracted with the water under 100℃ was the highest. But meanwhile, the type and the content of impurities was the highest, too. In addition, another six equal Microcystis aeruginosa was extract with the water under 100℃ for 5min, 10 min, 15 min, 20 min, 25 min and 30 min respectively. It was proved that 20 minutes was enough for extracting MC-LR from Microcystis aeruginosa, no long time was needed.
Different temperatures of water bath was used to extract the intracellular microcystin-LR(MC-LR) of Microcystis aeruginosa. Researching the extraction efficiency under the suitable temperature, so that it could find out the best temperature and time for extracting MC-LR from Microcystis aeruginosa cells. Five equal Microcystis aeruginosa was used to find out the best temperature, extracting at 60℃, 70℃, 80℃, 90℃ and 100℃ for 15 minutes, respectively. Results showed that the content of MC-LR extracted with the water under 100℃ was the highest. But meanwhile, the type and the content of impurities was the highest, too. In addition, another six equal Microcystis aeruginosa was extract with the water under 100℃ for 5min, 10 min, 15 min, 20 min, 25 min and 30 min respectively. It was proved that 20 minutes was enough for extracting MC-LR from Microcystis aeruginosa, no long time was needed.
Cite this paper
L. Fang, W. Liu, N. Zhao, J. Duan, Z. Wang, Y. Zhang, X. Xiao, J. Liu, G. Yin and C. Shi, "Studies on Extracting Microcystin-Lr from Microcystis Aeruginosa by Water Bath," Journal of Environmental Protection, Vol. 4 No. 1, 2013, pp. 70-73. doi: 10.4236/jep.2013.41B013.
L. Fang, W. Liu, N. Zhao, J. Duan, Z. Wang, Y. Zhang, X. Xiao, J. Liu, G. Yin and C. Shi, "Studies on Extracting Microcystin-Lr from Microcystis Aeruginosa by Water Bath," Journal of Environmental Protection, Vol. 4 No. 1, 2013, pp. 70-73. doi: 10.4236/jep.2013.41B013.
References
[1] ZHANG Ming-ming,PAN Gang,YAN Hai et al.A method to extract algae toxin of microcystin-LR[J].Journal of Environmental Sciences. 16(4):694-696.(2004) [2] REN Jing, WANG Xiang-rong, HAO Meng-li. Optimization of extracting microcystins in the cells of Microcystis aeruginosa with HPLC. Sciencepaper Online. [3] Jarkko Rapala, Kirsti Erkomaa, Jaana Kukkonen et al. Detection of microcystins with protein phosphatase inhibition assay, high-performance liquid chromatography-UV detection and enzyme-linked immunosorbent assay Comparison of methods. Analytica Chimica Acta. 466:213-231.(2002) [4] TANG Hong-feng, SUN Qian, MU Ting et al. The comparison of extracting microcystins in natural water bloom cyanobacteria[J]. Chemistry & Bioengineering. 28(1):82-84.(2011) [5] James S.Metcalf,Geoffrey A.Codd.Microwave oven and boiling waterbath extraction of hepatotoxins from cyanobacterial cells.FEMS Microbiology Letters.184:241-246.(2000) [6] LI Qiu-xia, CAI Chao-hai, XU Gui-lan. Rapid detection of microcystins-LR in drinking water with super fast performance liquid chromatography-tandem mass spectrometry. Chin J Health Lab Technol. 20(8):1875-1877.(2010) [7] LEI La-mei, Gan Nan-qin, SONG Li-rong. A rapid method to extract and analyse microcystins. ACTA Hydrobiologica Sinica. 27(5):468-471.(2003) [8] National Standards of Peoples Republic of China. GB/T 20466-2006. The detection of microcystins in water.
[1] ZHANG Ming-ming,PAN Gang,YAN Hai et al.A method to extract algae toxin of microcystin-LR[J].Journal of Environmental Sciences. 16(4):694-696.(2004) [2] REN Jing, WANG Xiang-rong, HAO Meng-li. Optimization of extracting microcystins in the cells of Microcystis aeruginosa with HPLC. Sciencepaper Online. [3] Jarkko Rapala, Kirsti Erkomaa, Jaana Kukkonen et al. Detection of microcystins with protein phosphatase inhibition assay, high-performance liquid chromatography-UV detection and enzyme-linked immunosorbent assay Comparison of methods. Analytica Chimica Acta. 466:213-231.(2002) [4] TANG Hong-feng, SUN Qian, MU Ting et al. The comparison of extracting microcystins in natural water bloom cyanobacteria[J]. Chemistry & Bioengineering. 28(1):82-84.(2011) [5] James S.Metcalf,Geoffrey A.Codd.Microwave oven and boiling waterbath extraction of hepatotoxins from cyanobacterial cells.FEMS Microbiology Letters.184:241-246.(2000) [6] LI Qiu-xia, CAI Chao-hai, XU Gui-lan. Rapid detection of microcystins-LR in drinking water with super fast performance liquid chromatography-tandem mass spectrometry. Chin J Health Lab Technol. 20(8):1875-1877.(2010) [7] LEI La-mei, Gan Nan-qin, SONG Li-rong. A rapid method to extract and analyse microcystins. ACTA Hydrobiologica Sinica. 27(5):468-471.(2003) [8] National Standards of Peoples Republic of China. GB/T 20466-2006. The detection of microcystins in water.