JBiSE  Vol.6 No.1 , January 2013
Influence of bone morphogenetic protein on articular cartilage regeneration following periosteal grafting
ABSTRACT

Background: Autologous periosteal grafting is used as treatment for articular cartilage defect. Objective: To study the effect of bone morphogenetic protein (BMP) on articular cartilage regeneration following periosteal grafting. Methods: 16 healthy 15 week-old New Zealand white rabbits of both sexes (32 knees) were randomly divided into experimental group (group A) and control group (group B). A4.0 mmdiameter full-thickness articular cartilage defect was created in the femoral intercondylar fossa in all rabbits. Following this, a4.0 mmdiameter section of the periosteum was harvested from the anteromedial part of the upper tibial bone. In group A (eight rabbits, 16 knees), the cartilage defect was covered with periosteum, into which 20 μg BMP and 20% Pluronic were injected. In group B (eight rabbits, 16 knees), the cartilage defect was covered with periosteum, into which the same dosage of 0.9% NS (Normal saline) and 20% Pluronic were injected. All rabbits were sacrificed at 4, 8, and 12 weeks postoperatively, the cartilage defect areas were examined macroscopically and microscopically, and the morphology of the chondrocytes and collagen fibers were examined by scanning electron microscopy. Results: The filling of the defects with regenerated tissue was observed in both the group. The most notable improvement was that the cartilage regeneration in group A was obviously superior to that in group B, with the total histological score in group A significantly higher. Conclusion: BMP is an effective factor that could promote regeneration of articular cartilage and lead to successful cartilaginous resurfacing following periosteal grafting


Cite this paper
Zhang, Y. , Jiang, X. and Guo, Y. (2013) Influence of bone morphogenetic protein on articular cartilage regeneration following periosteal grafting. Journal of Biomedical Science and Engineering, 6, 25-30. doi: 10.4236/jbise.2013.61004.
References
[1]   Bruder, S.P., Jaiswal, N. and Haynesworth, S.E. (1997) Growth kinetics,self-renewal,and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. Journal of Cellular Biochemistry, 64, 278-294. doi:10.1002/(SICI)1097-4644(199702)64:2<278::AID-JCB11>3.0.CO;2-F

[2]   Langer, R. and Vacanti, J.P. (1993) Tissue engineering. Science, 260, 920-926. doi:10.1126/science.8493529

[3]   O’Driscoll, S.W. and Fitzsimmons, J.S. (2001) The role of periosteum in cartilage repair. Clinical Orthopaedics and Related Research, 391, S190-S207. doi:10.1097/00003086-200110001-00019

[4]   Lietman, S.A., Yanagishita, M., Sampath, T.K. and Reddi, A.H. (1997) Stimulation of proteoglycan synthesis in explants of porcine articular cartilage by γOP-1(BMP7). The Journal of Bone & Joint Surgery, 79, 1132-1137.

[5]   Urist, M.R. (1965) Bone formation by autoinduction. Science, 150, 893-899. doi:10.1126/science.150.3698.893

[6]   Reddi, A.H. (1998) Role of morphogenetic proteins in skeletal tissue engineering and regeneration. Nature Bio- technology, 16, 247-252. doi:10.1038/nbt0398-247

[7]   Carranza-Bencano, A., García-Paino, L., Armas Padrón, J.R. and Cayuela Do-minguez, A. (2000) Neochondro- genesis in repair of full-thickness articular cartilage defects using free autogenous periosteal grafts in the rabbit. A follow-up in six months. Osteoarthritis Cartilage, 8, 351-358. doi:10.1053/joca.1999.0309

[8]   Wakitani, S., Goto, T., Pineda, S.J., Young, R.G., Man- sour, J.M., Caplan, A.I. and Goldberg, V.M. (1994) Mesenchymal cell-based repair of large full-thickness defects of articular cartilage. The Journal of Bone & Joint Surgery, 76, 592-597.

[9]   Marion, N.W. and Mao, J.J. (2006) Mesenchymal stem cells and tissue engineering. Methods in Enzymology, 420, 339-361. doi:10.1016/S0076-6879(06)20016-8

[10]   Oreffo, R.O., Cooper, C., Mason, C. and Clements, M. (2005) Mesen-chymal stem cells lineage, plasticity, and skeletal therapeutic potential. Stem Cell Reviews, 1, 169- 178. doi:10.1385/SCR:1:2:169

[11]   Leo, A.J. and Grande, D.A. (2006) Mesenchymal stem cells in tissue engineering. Cells Tissues Organs, 183, 112-122. doi:10.1159/000095985

[12]   Chen, F.H., Rousche, K.T. and Tuan, R.S. (2006) Technology Insight: adult stem cells in cartilage regeneration and tissue engineering. Nature Clinical Practice Rheu- matology, 2, 373-382. doi:10.1038/ncprheum0216

[13]   Luyten, F.P., Yu, Y.M., Yanagishita, M., Vukicevic, S., Hammonds, R.G. and Reddi, A.H. (1992)Natural bovine osteogenin and recom-binant BMP-2B are equipment in the maintenance of proteoglycans in bovine articular car- tilage explant cultures. Journal of Biological Chemistry, 267, 3685-3691.

[14]   Sung, M.S., Jeong, C.H., Lim, Y.S., Yoo, W.J., Chung, S.K. and Jung, N.Y. (2011) Periosteal autograft for articular cartilage defects in dogs: MR imaging and ultra-sonography of the repair process. Acta Radiologica, 52, 181-190. doi:10.1258/ar.2010.100087

[15]   Messner, K. and Gill-quist, J. (1996) Cartilage repair. A critical review. Acta Orthopaedica Scandinavica, 67, 523-529. doi:10.3109/17453679608996682

[16]   Sellers, R.S., Zhang, R., Glasson, S.S., Kim, H.D., Peluso, D., D’Augusta, D.A., Beckwith, K. and Morris, E.A. (2000) Repair of articular defects one year after treatment with recombinant human bone morphogenetic protein-2(rhBMP-2). The Journal of Bone & Joint Surgery, 82, 151-160.

[17]   Gallay, S.H., Miura, Y., Commisso, C.N., Fitzsimmons, J.S. and O’Driscoll, S.W. (1994) Relation-ship of donor site to chondrogenic potential of periosteum in vitro. Journal of Orthopaedic Research, 12, 515-525. doi:10.1002/jor.1100120408

[18]   O’Driscoll, S.W., Recklies, A.D. and Poole, A.R. (1994) Chondrogenesis in periosteal explants. An organ culture model for in vitro study. The Journal of Bone & Joint Surgery, 76A, 1042-1051.

[19]   Slynarski, K., Deszczynski, J. and Karpiski, J. (2006) Fresh bone marrow and periosteum transplantation for cartilage defects of the knee. Transplantation Proceedings, 38, 318-319. doi:10.1016/j.transproceed.2005.12.075

[20]   Olivos-Meza, A., Fitzsimmons, J.S., Casper, M.E., Chen, Q., An, K.-N., Ruesink, T.J., O’Driscoll, S.W. and Reinholz, G.G. (2010) Pretreatment of periosteum with TGF-β1 in situ enhances the quality of osteochondral tissue regenerated from transplanted periosteal grafts in adult rabbits. Os-teoarthritis Cartilage, 18, 1183-1191. doi:10.1016/j.joca.2010.06.003

[21]   Mahmut, A., Mithat, ?., Ahmet, G., Mehmet, H., O?uz, T. and ?zlem, C. (2010) The healing of full-thickness articular cartilage defects in rabbits: Successful results with fibroblast growth factor. Joint Diseases and Related Surgery, 21, 147-152.

[22]   Uluta?, K., Menderes, A., Karaca, C. and Ozkal, S. (2005) Repair of cartilage defects with periosteal grafts. The British Association of Plastic Surgeons, 58, 65-72. doi:10.1016/j.bjps.2004.05.010

[23]   Depalma, A.F., Mckeever, C.D. and Subin, D.K. (1996) Process of repair of articular cartilage demonstrated by histology and auto-radiography with tritiated thymidine. Clinical Orthopae-dics and Related Research, 48, 229- 242.

 
 
Top