AJAC  Vol.4 No.1 , January 2013
Isolation and Characterization of S-Enantiomer in Montelukast

A simple and rapid Supercritical Fluid Chromatography (SFC) Method has been developed to isolate and characterize the S-Isomer of Montelukast by using normal phase Amylose based AS-H with 250 mm × 30 mm, 5 microns column using a mobile phase system containing super critical fluid carbon dioxide (CO2) and the percentage of 2-Propanol as a mobile phase (85:15) and detection at 230 nm. The isolated S-Isomer was characterized by using FT-IR, ESI-MS, HPLC and 1H NMR. The purity of isolated S-Isomer is about 98%.

Cite this paper: V. Maddala, K. Kakumani, K. Chimalakonda, S. Polisetty and P. Ray, "Isolation and Characterization of S-Enantiomer in Montelukast," American Journal of Analytical Chemistry, Vol. 4 No. 1, 2013, pp. 56-61. doi: 10.4236/ajac.2013.41008.

[1]   ICH, “Validation of Analytical Procedures: Text and Metho-dology (Q2(R1)) International Conference on Harmonization,” IFPMA, Geneva, 2005.

[2]   L. Liu, H. Cheng, J. J. Zhao and J. D. Rogers, “Determination of Montelukast (MK-0476) and S-Enatiomer in Human Plasma by Stereoselective High Performance Liquid Chromatography with Column Switching,” Journal of Pharmaceutical and Biomedical Analysis, Vol. 15, No. 5, 1997, pp. 631-638. [Pub Med] doi:10.1016/S0731-7085(96)01894-8

[3]   B. Chauhan, S. Rani, M. Nivsarkar and H. Padh, “New Liquid-Liquid Extraction Method for Determination for Montelukast in Small Volume Human Plasma Samples Using HPLC with Fluorescence Detector,” Indian Journal of Pharmaceutical Science, Vol. 68, No. 4, 2006, pp. 517-520. doi:10.4103/0250-474X.27834

[4]   N. M. Maier, P. Franco and W. Lindner, “Separation of Enan-tiomers: Needs Challenges, Perspectives,” Journal of Chromatography A, Vol. 906, No. 1-2, 2001, pp. 3-33. doi:10.1016/S0021-9673(00)00532-X

[5]   V. Choudari, et al., “Simultaneous Determination of Montelukast Sodium and Levocitrazine Dihydrochloride in Pharmaceutical Preparations by Ratio Derivative Spectroscopy,” International Journal of PharmTech Research, Vol. 2, No. 1, 2010, p. 4.

[6]   P. Rad-hakrishnan, D. V. Subbarao, K. V. Surendranath, D. Subrah-manyam and V. Himabindu, “A Validated LC Method for Determination of the Enantiomeric Purity of Montelukast Sodium in Bulk Drug Samples and Pharma- ceutical Dosage Forms,” Chromatographia, Vol. 68, No. 3-4, 2008, pp. 263-267.

[7]   B. S. Sekhon, “Separation of Pharmaceutical Enantiomers Using Supercritical Fluid Technology,” International Journal of Pharmtech Research, Vol. 2, No. 2, 2010, pp. 1595-1602.