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 AJAC  Vol.4 No.1 , January 2013
Development and Validation of New RP-HPLC Method for the Estimation of Alfuzosin Hydrochloride in Bulk and Tablet Dosage Form
Abstract: A reverse phase high performance liquid chromatographic method (HPLC) has been developed for the estimation of Alfuzosin hydrochloride (ALH) in the pharmaceutical formulation using RP-C18 column. The mobile phase (Tetrahydrofuran, Acetonitrile and buffer (pH 3.50) was pumped at a flow rate of 1.5 ml/min in the ratio of 1:20:80 and the eluents were monitored at 254.0 nm. Linearity was obtained in the concentration range of 80 - 120 μg/mL for ALH. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method may be used for determination of Alfuzosin hydrochloride in bulk drugs and in pharmaceutical dosage form.
Cite this paper: V. Patil, S. Devdhe, S. Kale, V. Nagmoti, S. Kurhade, Y. Girbane and M. Gaikwad, "Development and Validation of New RP-HPLC Method for the Estimation of Alfuzosin Hydrochloride in Bulk and Tablet Dosage Form," American Journal of Analytical Chemistry, Vol. 4 No. 1, 2013, pp. 34-43. doi: 10.4236/ajac.2013.41005.
References

[1]   D. Yu, et al., “Research Highlights,” Prostate News Prostate Cancer and Prostatic Diseases, Vol. 9, 2006, pp. 2-5.

[2]   S. A. Mc Neill, T. B. Hargreave, C. Geffriaud-Ricouard, J. Santoni and C. G. Roehrborn, “Postvoid Residual Urine in Patients with Lower Urinary Tract Symptoms Suggestive of Benign Prostatic Hyperplasia: Pooled Analysis of Eleven Controlled Studies with Alfuzosin,” Urology, Vol. 57, No. 3, 2001, pp. 459-465.

[3]   M. Vamsi Krishna and D. Gowri Sankar, “Optimization and Validation of Quantitative Spectrophotometric Meth- ods for the Determination of Alfuzosin in Pharmaceutical Formulations,” E-Journal of Chemistry, Vol. 4, No. 3, 2007, pp. 397-407.

[4]   M. Vamsi Krishna and D. Gowri Sankar, “New Diazo Coupling Reactions for Visible Spectrometric Determination of Alfuzosin in Pharmaceutical Preparations,” E-Journal of Chemistry, Vol. 4, No. 4, 2007, pp. 496-501.

[5]   M. Vamsi Krishna and D. Gowri Sankar, “Oxidative Coupling, Complex Formation and Internal Salt Formation Reactions for Visible Spectrophotometric Determination of Alfuzosin Hydrochloride in Pharmaceutical Formulations,” The Pharma Review, June-July2007, pp. 143-145.

[6]   A. Safwan, C. Fawaz and B. Roula, “Spectrophotometric determination of Alfuzosin Hydrochloride in Pharmaceutical Preparations with Some Sulphonepthelein Dyes,” International Journal of Biomedical Science, Vol. 2, No. 3, 2006, p. 273-278.

[7]   B. M. Ishaq, K. V. Prakash, C. H. Kumar, G. U. Rani and P. Ramakrishna, “Colorimetric Determination of Alfuzosin HCl in Pharmaceutical Formulations,” Journal of Pharmacy Research, Vol. 4, No. 1, 2011, pp. 226-228.

[8]   B. Uslu, “Voltammetric Analysis of Alfuzosin HCl in Pharmaceuticals, Human Serum and Simulated Gastric Juice,” Electroanalysis, Vol. 14, No. 12, 1990, pp. 866-870.

[9]   A. Rouchouse, M. Manoha, A. Durand and J. P. Thenot, “Direct High Performance Liquid Chromatographic Determination of the Enantiomers of Alfuzosin in Plasma on a Second-Generation Α1-Acid Glycoprotein Chiral Stationary Phase,” Journal of Chromatography A, Vol. 506, 1990, pp. 601-610.

[10]   P. Guinebault, M. Broquaire, C. Colafranceschi and J. P. Thénot, “High-Performance Liquid Chromatographic Determination of Alfuzosin in Biological Fluids with Fluorimetric Detection and Large-Volume Injection,” Journal of Separation Science, Vol. 29, No. 15, 2011, pp. 2303-2309.

[11]   K. S. B. Kumar, V. A. Ranjani and D. Sathyavathi, “New RP-HPLC Method Development and Validation for Assay of Alfuzosin Hydrochloride in Tablet Dosage Form,” International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 2, No. 4, 2010, pp. 90-92.

[12]   G. Mani, S. Uppatyay, R. Tivari, K. Kalliappan, R. Govindasamy, S. Gangully and S. Thangavel, “Quantitation of Alfuzosin Hydrochloride in Pharmaceutical Formulations by RP-HPLC,” Pakistan Journal of Pharmaceutical Sciences, Vol. 22, No. 3, 2009, pp. 263-266.

[13]   S. A. Raju, A. B. Karadi and S. Manjunath, “Reverse Phase HPLC Method for the Analysis of Alfuzosin Hydrochloride in Pharmaceutical Dosage Forms,” International Journal of Chemical Sciences, Vol. 6, No. 1, 2008, pp. 399-404.

[14]   G. Carlucci, E. Di Giuseppe and P. Mazzeo, “Determination of Alfuzosin in Human Plasma by High-Performance Liquid Chromatography with Column-Switching,” Journal Liquid Chromatography and Related Technologies, Vol. 17, No. 18, 1994, pp. 3989-3997. doi:10.1080/10826079408016168

[15]   J. L. Wiesner, F. C. W. Sutherland, G. H. Van Essen, H. K. L. Hundt, K. J. Swart and F. Hundt, “Selective, Sensitive and Rapid Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Alfuzosin in Human Plasma,” Journal of Chromatography B, Vol. 788, No. 2, 2003, pp. 361-368. doi:10.1016/S1570-0232(03)00064-3

[16]   D. B. Patel and N. J. Patel, “Development and Validation of Reverse Phase High Performance Liquid Chromatography and High Performance Thin Layer Chromatography Methods for Estimation of Alfuzosin Hydrochloride in Bulk and in Pharmaceutical Formulations,” International Journal of ChemTech Research, Vol. 1, No. 4, 2009, pp. 985-990.

[17]   A. S. Fayed, M. A. Shehata, N. Y. Hassan and S. A. El Weshahy, “Validated HPLC and HPTLC Stability-Indicating Methods for Determination of Alfuzosin Hydrochloride in Bulk Powder and Pharmaceutical Formulations,” Journal Of Separation Science, Vol. 29, No. 18, 2006, pp. 2716-2724. doi:10.1002/jssc.200600220

[18]   S. S. Deshmukh, S. S. Havele, V. V. Musale and S. R. Dhaneshwar, “Development and Validation of RP-HPLC Method for Simultaneous Estimation of Alfuzosin Hydrochloride and Dutasteride in Pharmaceutical Dosage Form,” Scholars Research Library, Vol. 2, No. 6, 2010, pp. 342-349.

[19]   Ch. A. Varshini, K. S. Kumari, S. Sushma and K. Prakash, “Development And Validation Of RP-HPLC Method For Simultaneous Estimation Of Alfuzosin Hydrochloride and Dutasteride in Bulk and Pharmaeutical Dosage Form,” Inventi Rapid: Pharm Analysis & Quality Assurance, Vol. 2012, No. 4, 2012.

[20]   K. S. B. Kumar, V. A. Ranjani and D. Sathyavathi, “New RP-HPLC Method Development and Validation for Assay of Alfuzosin Hydrochloride in Tablet Dosage Form,” International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 2, No. 4, 2010, pp. 90-92.

 
 
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