Back
 ENG  Vol.4 No.10 , October 2012
Conjugation of the Recombinant Third Domain of Human Alpha-fetoprotein with Doxorubicin Using Poly(amidoamine) Dendrimers and Study of Its Cytotoxic Activity
Abstract: The recombinant third domain of alpha-fetoprotein (AFP3D) is one of the promising vectors for targeted delivery of anticancer drugs to tumor cells. AFP3D-based conjugates with various anti-tumor agents have shown specific anti-tumor activity. The content of anti-tumor drugs in the preparation could be increased by including dendrimers - highly branched nanoparticles - into a targeted delivery system. In this study, we synthesized the AFP3D conjugate with doxorubicin-loaded poly(amidoamine) (PAMAM) dendrimer nanoparticles and investigated its cytotoxic activity. 
Cite this paper: S. Kisil, V. Chernikov, M. Danilevskiy, L. Savvateeva and N. Gorokhovets, "Conjugation of the Recombinant Third Domain of Human Alpha-fetoprotein with Doxorubicin Using Poly(amidoamine) Dendrimers and Study of Its Cytotoxic Activity," Engineering, Vol. 4 No. 10, 2012, pp. 80-83. doi: 10.4236/eng.2012.410B021.
References

[1]   B.F.Crandall, Alpha-fetoprotein, CRC Rev. Clin. Lab. Sci., vol. 15, pp. 127-185, 1981.

[2]   R. Chaturvedi, V. Agarkar, G. L. Sharma, P.U. Sarma, Purification of alpha-fetoprotein from human cord blood, Prep. Biochem. Biotechnol., pp. 293-303, April 1998.

[3]   H. F. Deutsch, Chemisry and Biology of α-fetoprotein, Adv. Cancer Res., vol.56, 1991, pp. 253-312.

[4]   S. Nishi, H. Hirai, Purification of human, dog and rabbit α-fetoprotein by immunoadsorbents of Sepharose coupled with anti-human α-fetoprotein, Biochim. Biophys. Acta, vol. 278, pp. 293-298, 1972.

[5]   R. Boismenu, D. Semeniuk, R. A. Murgita, Purification and characterization of human and mouse recombinant alpha-fetoproteins expressed in Escherichia coli, Protein Expression and Purif., vol. 10, pp.10-26, 1997.

[6]   Posypanova G. A., Gorokhovets N. V., Makarov V. A., Savvateeva L. V., Kireeva N. N., Severin S. E., Severin E. S., Recombinant alpha-fetoprotein C-terminal fragment: the new recombinant vector for targeted delivery. J Drug Target., vol.16, pp. 321-328, May 2008.

[7]   Savvateeva L. V., Gorokhovets N. V., Makarov V. A., Posypanova G. A., Severin S. E., Recombinant third domain of human alpha-fetoprotein: selectivity and antitumor activity of conjugate with Taxol. Abstracts of the XXXIII Meeting of the International Society for Oncodevelopmental Biology and Medicine (ISOBM), Rhodes, Greece, p. 84., September 2005.

 
 
Top