AMPC  Vol.2 No.4 , December 2012
In Vitro Study of Carbamate-Linked Cationic Lipid for Gene Delivery Against Cervical Cancer Cells
Abstract: Design and synthesis of a carbamate-linked cationic lipid DDCTMA (N-[1-(2,3-didodecylcarbamoyloxy)propyl]-N,N,N-trimethylammonium iodide)  as gene delivery carriers was described in this work. The transfection efficiency of cationic liposome increased dramatically with the increase in the content of DOPE. In addition, the transfection efficiency of some of cationic lipoplexes was superior or parallel to that of two commercial transfection agents, Lipofectamine2000 and DOTAP. The carbamate-linked cationic lipid DDCTMA/DOPE may be a promising gene carrier that has high transfection efficiency as well as low cytotoxicity.
Cite this paper: D. Zhi, S. Zhang, Y. Zhao, S. Cui, B. Wang, H. Chen, D. Zhi and D. Zhao, "In Vitro Study of Carbamate-Linked Cationic Lipid for Gene Delivery Against Cervical Cancer Cells," Advances in Materials Physics and Chemistry, Vol. 2 No. 4, 2012, pp. 229-232. doi: 10.4236/ampc.2012.24B058.

[1]   L. Ciani, S. Ristori, A. Salvati, L. Calamai and G. Martini, “DOTAP/DOPE and DC-Chol/DOPE lipoplexes for gene delivery: zeta potential measurements and electron spin resonance spectra,” Biochim. Biophys. Acta. vol. 1664, pp. 70-79, July 2004.

[2]   P. L., Felgner and G. M. Ringold, “Cationic liposomemediated transfection,” Nature. vol. 337, pp. 387-388, January 1989.

[3]   M. A., Mintzer and E. E., Simanek, “Nonviral vectors for gene delivery,” Chem. Rev. vol. 109, pp. 259-302, April 2009.

[4]   D. F. Zhi, S. B. Zhang, B. Wang, Y. N. Zhao, B. L. Yang and S. J. Yu, “Transfection efficiency of cationic lipids with different hydrophobic domains in gene delivery,” Bioconjugate Chem. vol. 21, pp. 563-577, January 2010.

[5]   D. F. Zhi, F. Qureshi, S. B. Zhang, Y. N. Zhao, S. H. Cui, B. Wang, H. Y. Chen, Y. H. Wang and D. F. Zhao, “Synthesis and biological activity of carbamate-linked cationic lipids for gene delivery in vitro,” Bioorg. Med. Chem. Lett., vol. 22, pp. 3837-3841, February 2012.

[6]   T. Yoshioka, S. Yoshida, T. Kurosaki, M. Teshima, K. Nishida, J. Nakamura, M. Nakashima, H. To, T. Kitahara and H. Sasaki, “Cationic liposomes-mediated plasmid DNA delivery in murine hepatitis induced by carbon tetrachloride,” J. Liposome Res. vol. 19, pp. 141-147, June 2009.

[7]   S., Fletcher, A., Ahmad, E., Perouzel, M. R., Jorgensen and A. D. Miller, “A dialkanoyl analogue of DOPE improves gene transfer of lower-charged, cationic lipoplexes.” Org. Biomol. Chem., vol. 4, pp. 196-199, December 2006.