ENG  Vol.4 No.10 , October 2012
Red Blood Cell Mechanical Stability
Author(s) Oguz K. Baskurt*
Abstract
It has been well documented that shear forces effective above a certain magnitude under flow conditions causes damage in blood cells. This damage ranges from slight morphological alteration to the destruction of red blood cells (RBC). The hemolytic trauma to RBC can easily be detected by measuring free hemoglobin level in plasma, however there are no standardized protocols to quantitate subhemolytic trauma. Ektacytometry has been used to study the alterations in RBC mechanical properties induced by the application of shear stress at various levels. Additionally, a protocol for measuring the hemolytic threshold as an indicator of subhemolytic damage to RBC has been developed based on ektacytometry. These standardized protocols may find applications in the pre-clinical and clinical evaluation of artificial organs and biomedical devices contacting with blood.

Cite this paper
O. Baskurt, "Red Blood Cell Mechanical Stability," Engineering, Vol. 4 No. 10, 2012, pp. 8-10. doi: 10.4236/eng.2012.410B003.
References

[1]   T. Alexy, O.K. Baskurt, N. Nemeth, M. Uyuklu, R.B. Wenby and H.J. Meiselman, Effect of lanthanides on red blood cell deformability and response to mechanical stress: role of lanthanide ionic radius, Biorheology 48 (2011), 173-183.

[2]   O.K. Baskurt, M. Uyuklu and H.J. Meiselman, Protection of erythrocytes from sub-hemolytic mechanical damage by nitric oxide mediated inhibition of potassium leakage, Biorheology 41 (2004), 79-89.

[3]   D.E. Brinsfield, M.A. Hoff, R.B. Geering and P.M. Galletti, Hematological changes in long term perfusion, J Appl Physiol 17 (1962), 531-538.

[4]   R.G. Cooper, R.A. Kahn, C.N. Cornell and M.E. Muhrer, Erythrocyte mechanical fragility test, J Clin Pathol 21 (1968), 781-783.

[5]   S. Deutsch, J.M. Tarbell, K.B. Manning, G. Rosenberg and A.A. Fontaine, Experimental fluid mechanics of pulsatile artificial blood pumps, Ann Rev Fluid Mech 38 (2006), 65-86.

[6]   M. Giersiepen, L.J. Wurzinger, R. Opitz and H. Reul, Estimation of shear stress-related blood damage in heart valve prostheses- in vitro comparison of 25 aortic valve, Artif Organs 13 (1990), 300-306.

[7]   J.M. Greve, A.S. Les, B.T. Tang, M.T. Draney Blomme, N.M. Wilson, R.L. Dalman, N.J. Pelc and C.A. Taylor, Allometric scaling of wall shear stress from mice to humans: quantification using cine phase-contrast MRI and computational fluid dynamics, Am J Physiol Heart Circ Physiol 291 (2006), H1700-H1708.

[8]   L. Gu, W.A. Smith and G.P. Chatzimavroudis, Mechanical fragility calibration of red blood cells, Asaio J 51 (2005), 194-201.

[9]   M.V. Kameneva and J.F. Antaki, Mechanical trauma to blood, in: O.K.Baskurt, M.R.Hardeman, M.W.Rampling and H.J.Meiselman (Eds.), Handbook of Hemorheology and Hemodynamics, Amsterdam, Berlin, Oxford, Tokyo, Washington DC, 2007, pp. 206-227.

[10]   S.S. Lee, K.H. Ahn, S.J. Lee, K. Sun, P.T. Goedhart and M.R. Hardeman, Shear induced damage of red blood cells monitored by the decrease of their deformability, Korea-Australia Rheology Journal 16 (2004), 141-146.

[11]   P.J. Marascalco, S.P. Ritchie, T.A. Snyder and M.V. Kameneva, Development of standard tests to examine viscoelastic properties of blood of experimental animals for pediatric mechanical support device evaluation, Asaio J 52 (2006), 567-574.

[12]   C.G. Nevaril, E.C. Lynch, C.P. frey Jr and J.D. Hellums, Erythrocyte damage and destruction induced by shear stress, J Lab Clin Med 71 (1968), 784-790.

[13]   E.A. Orear, M.M. Udden, J.A. Farmer, L.V. McIntire and E.C. Lynch, Increased Intracellular Calcium and Decreased Deformability of Erythrocytes from Prosthetic Heart-Valve Patients, Clinical Hemorheology 4 (1984), 461-471.

[14]   J.G. Sandza, R.E. Clark, C.S. Weldon and S.P. Sutera, Subhemolytic trauma of erythrocytes; recognition and sequestration by spleen as a function of shear, ASAIO Trans 2 (1974), 457-462.

[15]   S.P. Sutera, Flow-induced trauma to blood cells, Circ Res 41 (1977), 2-8.

 
 
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