Health  Vol.2 No.9 , September 2010
Beneficial effect of reduced oxygen concentration with transfer of blastocysts in IVF patients older than 40 years old
ABSTRACT
The aim of the present study was to determine the impact of oxygen concentration on implantation, pregnancy and delivery rates in IVF patients older than 40 year old with transfer of blastocysts. Included were 558 women aged 23-45 years old undergoing IVF/ICSI procedures whose embryos were cultured at blastocyst stage under two different oxygen environments (a bi-gas system: 5.6% CO2 in air and a tri-gas system: 5.6% CO2, 5% de O2 and 89.4% N2). The main outcome measures of this study are implantation, pregnancy and delivery rates. Implantation, pregnancy and delivery rates are found to be reduced in women older than 40 years old. The implantation and pregnancy rates are significantly higher in women older than 40 years old from the 5% of O2 group, in comparison to the 20% group (25.00% versus 2.70% and 41.38% versus 5.56%; P < 0.05). The deliveries rates were 13.79% and 5.56% in the 5% and 20% oxygen groups respectively (P: NS). The birthweight was similar in both study groups (P: NS). Gestational age was significantly longer in wo- men from the 5% of O2 group, in comparison to the 20% (36.87 versus 35.87 weeks, P < 0.05). Results indicated that the embryonic culture with 5% of oxygen and transfer of blastocysts in women older than 40 years old improve the results in the in Vitro fertilization/intracytoplasmic injection procedures (IVF/ICSI).

Cite this paper
nullGarcía, J. , Sepúlveda, S. and Noriega-Hoces, L. (2010) Beneficial effect of reduced oxygen concentration with transfer of blastocysts in IVF patients older than 40 years old. Health, 2, 1010-1017. doi: 10.4236/health.2010.29149.
References
[1]   Bayatt-Smith, J.G., Leese, H.J. and Gosden, R.G. (1991) An investigation by mathematical modeling of whether Mouse and human preimplantation embryos in static culture can satisfy their demands for oxygen by diffusion. Human Reproduction, 6(1), 52-57.

[2]   Fisher, B. and Bavister, B.D. (1993) Oxygen tension in the oviduct and uterus of rhesus monkeys, hamsters and rabbits. Journal of Reproduction and Fertility, 99(2), 673-679.

[3]   Yedwab, G.A., Paz, G., Homonnai, T.Z., David, M.P. and Kraicer, P.F. (1976) Temperature, pH and partial pressure of oxygen in the cervix and uterus of women and uterus of rats during the cycle. Fertility and Sterility, 27(3), 304-309.

[4]   Johnson, M.H. and Nasr-Esfahani, M.H. (1994) Radical solutions and cultural problems: Could free oxygen radicals be responsible for the impaired development of pre- implantation mammalian embryos in vitro? Bioassays, 16(1), 31-38.

[5]   Catt, J.W. and Henman, M. (2000) Toxic effects of oxygen on human embryo development. Human Reproduction, 15(Suppl. 2), 199-206.

[6]   Bavister, B.D. (1995) Culture of preimplantation embryos: Facts and artifacts. Human Reproduction Update, 1(2), 91-148.

[7]   Karagenc, L., Sertkaya, Z. and Ciray, N. (2004) Impact of oxygen concentration on embryonic development of mouse zygotes. Reproductive BioMedicine Online, 9(4), 409-417.

[8]   Pabon, J.E., Jr, Findley, W.E. and Gibbons, W.E. (1889) The toxic effect of short exposures to the atmospheric oxygen concentration on early mouse embryonic development. Fertility and Sterility, 51(5), 896-900.

[9]   Umaoka, Y., Noda, Y. and Narimoto, K. (1992) Effects of oxygen toxicity on early development of Mouse embryos. Molecular Reproduction and Development, 31(1), 28-33.

[10]   Thompson, J.G., Simpson, A.C., Pugh, P.A., Donnelly, P.E. and Tervit, H.R. (1990) Effect of oxygen concentration on in-vitro development of preimplantation sheep and cattle embryos. Journal of Reproduction and Fertility, 89(2), 573-578.

[11]   Li, J., Foote, R.H. and Simkin, M. (1993) Development of rabbit zygotes cultured in protein-free medium with catalase, taurine or superoxide dismutase. Biology of Reproduction, 49(1), 33-37.

[12]   McKiernan, S.H. and Bavister, B.D. (1990) Environmental variables influencing in vitro development of hamster 2 cell embryos to the blastocyst stage. Biology of Reproduction, 43(3), 404-413.

[13]   Kishi, J., Noda, Y. and Narimoto, K. (1998) Block to development in cultured rat 1-cell embryos is overcome using medium HECM-1. Human Reproduction, 6(10), 1445-1448.

[14]   Fukui, Y., McGrowan, L.T. and James, R.W. (1991) Factors affecting the in-vitro development to blastocysts of bovine oocytes matured and fertilized in vitro. Journal of Reproduction and Fertility, 92(1), 125-131.

[15]   Kitagawa, Y., Suzuki, K. and Yoneda, A. (2004) Effects of oxygen concentration and antioxidants on the in vitro developmental ability, production of reactive oxygen species (ROS) and DNA fragmentation in porcine embryos. Theriogenology, 62(7), 1186-1197.

[16]   Dumoulin, J., Vanvuchelen, R. and Land, J. (1995) Effect of oxygen concentration on in-vitro fertilization and embryo transfer. Fertility and Sterility, 63(1), 115-119.

[17]   Bah?eci, M., ?iray, H.N., Karagenc, L., Ulu?, U. and Bener, F. (2005) Effect of oxygen concentration during the incubation of embryos of women undergoing ICSI and embryo transfer: A prospective randomized study. Reproductive BioMedicine Online, 11(4), 438-443.

[18]   Dumoulin, J.C.M., Meijers, C.J.J. and Bras, M. (1999) Effect of oxygen concentration on human in-vitro fertilization and embryo culture. Human Reproduction, 14(2), 465-469.

[19]   Waldenstr?m, U., Engstr?m, A., Hellberg, D., Nilsson, S. (2009) Low-oxygen compared with high-oxygen atmosphere in blastocyst culture, a prospective randomized study. Fertility and Sterility, 91(6), 2461-2465.

[20]   Meintjes, M., Chantilis, S., Douglas, J., Rodriguez, A., Guerami, A., Bookout, D., Barnett, B. and Madden, J. (2009) A controlled randomized trial evaluating the effect of lowered incubator oxygen tension on live births in a predominantly blastocyst transfer program. Human Reproduction, 24(2), 300-307.

[21]   Gardner, D.K., Lane, M., Calderon, I. and Leeton, J. (1996) Environment of the preimplantation human embryo in vivo: Metabolite analysis of oviduct and uterine fluids and metabolism of cumulus cells. Fertility and Sterility, 65(2), 349-53.

[22]   Fanchin, R., Ayoubi, J.M., Righini, C., Olivennes, F., Schonauer, L.M. and Frydman, R. (2001) Uterine contractility decreases at the time of blastocyst transfers. Human Reproduction, 16(6), 1115-1119.

[23]   Quea, G., Romero, K., García-Velasco, J.A. (2007) Extended embryo culture to increase implantation rate. Reproductive Biomedicine Online, 14(3), 375-383.

[24]   Kea, B., Gebhardt, J., Watt, J., Westphal, L.M., Lathi, R.B. and Milki, A.A. (2007) Effect of reduced oxygen concentrations on the outcome of in vitro fertilization. Fertility and Sterility, 87(1), 213-216.

[25]   Kova?i?, B. and Vlaisavljevi?, V. (2008) Influence of atmospheric versus reduced oxygen concentration on development of human blastocysts in vitro: a prospective study on sibling oocytes. Reproductive BioMedicine Online, 2(2), 229-236.

[26]   Kova?i?, B., Sajko, M. and Vlaisavljevi?, V. (2010) A prospective randomized trial on the effect of atmospheric versus reduced oxygen concentration on the outcome on intracytoplasmic sperm injection cycles. Fertility and Sterility, 94(2), 511-519.

[27]   Nanassy, L., Peterson, A., Wilcox, A., Peterson, M., Hammoud, A. and Carrell, D. (2010) Comparison of 5% and ambient oxygen during days 3-5 in vitro culture of human embryos. Fertility and Sterility, 93(2), 579-585.

[28]   Perheentupa, A. and Huhtaniemi, I. (2009) Aging of the human ovary and testis. Journal of Molecular Cell and Endocrinology, 299(1), 2-13.

[29]   Steiner, A.Z. (2009) Clinical implications of ovarian reserve testing. Obstetrical and Gynecological Survey, 64(2), 120-128.

[30]   Sauer, M.V., Paulson, R.J. and Lobo, R.A. (1992) Reversing the natural decline in human fertility. An extended clinical trial of oocytes donation to women of advanced reproductive age. Journal of the American Medical Association, 268(10), 1275-1279.

[31]   Marcus, S.F. and Brinsden, P.R. (1996) In-vitro fertilization and embryo transfer in women aged 40 years and over. Human Reproduction Update, 2(6), 459-468.

[32]   Registro Latinoamericano de Reproducción Asistida (2006) Editado por la Red Latinoamericana de Repro- ducción Asistida. Redlara, Chile, 1-95.

[33]   Bar-Hava, I., Orvierto, R., Ferber, A., Ashkenazi, J., Dicker, D. and Ben-Rafael, Z. (1999) Standard in vitro fertilization or intracytoplasmic sperm injection in advanced female age, what may be expected? Gynecology and Endocrinology, 13(2), 93-97.

[34]   Munné, S., Cohen, J. and Sable, D. (2002) Preimplantation genetic diagnosis for advanced maternal age and other indications. Fertility and Sterility, 78(2), 234-236.

[35]   Munné, S., Alikani, M., Tomkin, G., Grifo, J. and Cohen, J. (1995) Embryo morphology, developmental rates, and maternal age are correlated with chromosomal abnormalities. Fertility and Sterility, 64(2), 382-391.

[36]   Nybo Andersen, A.M., Wohlfahrt, J., Christens, P., Olsen, J. and Melbye, M. (2000) Maternal age and fetal loss: Population based register linkage study. British Medical Journal, 320(7251), 1708-1712.

[37]   Ciray, H.N., Aksoy, T., Yaramanci, K., Karayaka, I. and Bah?eci, M. (2009) In vitro culture under physiologic oxygen concentration improves blastocyst yield and quality: a prospective randomized survey on sibling oocytes. Fertility and Sterility, 91(Suppl. 1), 1459-1461.

[38]   García, J.I., Gonzales, G.F. and Noriega-Hoces, L. (2007) Sperm chromatin stability and its relationship with fertilization rate after Intracytoplasmic Sperm Injection (ICSI) in an assisted reproduction program. Journal of Assisted Reproduction and Genetics, 24(12), 587-593.

[39]   Mansour, R. (2005) Minimizing embryo expulsion after embryo transfer: A randomized controlled study. Human Reproduction, 20(1), 170-174.

[40]   Gardner, D., Lane, M. (2001) Embryo culture in textbook of assisted reproductive techniques: laboratory and clinical perspectives. In: Gardner, D., Weissman, A., Howles, C. and Shoham, Z., Eds., Martin Dunitz Press, London, 203-222.

[41]   Burton, G.J., Hempstock, J. and Jauniaux, E. (2000) Oxygen, early embryonic metabolism and free radical-mediated embryopathies. Reproductive Biomedicine Online, 6(1), 84- 96.

[42]   Adam, A.A., Takahashi, Y., Katagiri, S. and Nagano, M. (2004) Effects of oxygen in the gas atmosphere during in vitro maturation, in vitro fertilization and in vitro culture on the efficiency of in vitro production of mouse embryos. Japan Journal Veterinary Research, 52(2), 77-84.

[43]   Quinn, P. and Harlow, G.M. (1978) The effect of oxygen on the development of preimplantation mouse embryo in vitro. Journal of Experimental Zoology, 206(1), 73-80.

[44]   Takahashi, Y. and Kanagawa, H. (1998) Effect of oxygen concentration in the gas atmosphere during in vitro insemination of bovine oocytes on the subsequent embryonic development in vitro. Journal of Veterinary Medical Science, 60(3), 365-367.

[45]   Farrell, P.B. and Foote, R.H. (1995) Beneficial effects of culturing rabbit zygotes to blastocysts in 5% oxygen and 10% carbon dioxide. Journal of Reproduction and Fertility, 103(1), 127-130.

[46]   Im, G.S., Lai, L., Liu, Z., Hao, Y., Wax, D., Bonk, A. and Prather, R.S. (2004) In vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres. Theriogenology, 61(6), 1125-1135.

[47]   Bavister, B.D. (2004) The role of animal studies in supporting human ART. Reproduction Fertility and Development, 16(7), 719-728.

[48]   Rho, G.J., Kim, D.S., Son, W.J., Cho, S.R., Kim, J.G., MK, B. and Choe, S.Y. (2007) Influence of in vitro oxygen concentrations on preimplantation embryo development, gene expression and production of Hanwoo calves following embryo transfer. Molecular Reproduction and Development, 74(4), 486-496.

[49]   Rinaudo, P.F., Giritharan, G., Talbi, S., Dobson, A.T., Schultz, R.M. (2006) Effects of oxygen tension on gene expression in preimplantation mouse embryos. Fertility and Sterility, 86(Suppl. 4), 1252-1265.

[50]   Umaoka, Y., Noda, Y. and Narimoto, K. (1991) Developmental potentiality of embryos cultured under low oxygen tension with superoxide dismutase. Journal of In Vitro Fertilization and Embryo Transfer, 8(5), 245-249.

[51]   Chang, H.J., Lee, J.R., Jee, B.C., Suh, C.S. and Kim, S.H. (2009) Impact of blastocyst transfer on offspring sex ratio and the monozygotic twinning rate: A systematic review and meta-analisys. Fertility and Sterility, 91(6), 2381-2390.

[52]   Beesley, R., Robinson, R., Propst, A., Arthur, N. and Retzloff, M. (2009) Impact of day 3 or day 5 embryo transfer on pregnancy rates and multiple gestations. Fertility and Sterility, 91(5), 1717-1720.

[53]   Shapiro, B., Richter, K.S., Harris, D.C. and Daneshmand, S. (2002) Influence of patient age on the growth and transfer blastocyst-stage embryos. Fertility and Sterility, 77(4), 700-705.

[54]   Munné, S., Chen, S., Colls, P., Garrisi, J., Zheng, X., Cekleniak, N., Lenzi, M., Hughes, P., Fisher, J., Garrisi, M., Tomkin, G. and Cohen, J. (2007) Maternal age, morphology, development and chromosome abnormalities in over 6000 cleavage-stage embryos. Reproductive Biomedicine Online, 14(5), 628-634.

[55]   Munné, S., Bah?e, M., Sandalinas, M., Escudero, T., Márquez, C., Velilla, E., Colls, P., Oter, M., Alikani, M. and Cohen, J. (2004) Differences in chromosomes susceptibility to aneuploidy and survival to first trimester. Reproductive Biomedicine Online, 8(1), 81-90.

[56]   Magli, M.C., Gianaroli, L. and Ferraretti, A.P. (2001) Chromosomal abnormalities in embryos. Molecular and Cellular Endocrinology, 183(Suppl. 1), S29-S34.

[57]   Bielanska, M., Tan, S.L. and Ao, A. (2002a) Chromosomal mosaicism throughout human preimplantation development in vitro: incidence, type and relevance to embryo outcome. Human Reproduction, 17(2), 413-419.

[58]   Gianaroli, L., Magli, M.C. and Ferraretti, A.P. (2007) Oocyte euploidy, pronuclear zygotes morphology and embryo chromosomal complement. Human Reproduction, 22(1), 241-249.

[59]   Munné, S. (2006) Chromosome abnormalities and their relationship to morphology and development of human embryos. Reproductive Biomedicine Online, 12(2), 234- 253.

[60]   Fragouli, E., Alfarawati, S., Katz-Jaffe, M., Alfarawati, S., Stevens, J., Colls, P., Goodall, N.N., Tormasi, S., Gutierrez- Mateo, C., Prates, R., Schoolcraft, W.B., Munné, S. and Wells, D. (2010) Comprehensive chromosome screening of polar bodies and blastocysts from couples experiencing repeated implantation failure. Fertility and Sterility, 94(3), 875-887.

 
 
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