The present study was
designed to explore the possible mechanism of obesity associated metabolic
syndrome. 150 subjects (120 men and 30 women) in the age-group of 17 - 26 years
were studied. Body Mass Index and Waist-to-Hip Ratio were taken as a measure of
generalized obesity and abdominal adiposity. The serum concentration of
glucose-6-phosphate dehydrogenase increased with increasing levels of Body
Mass Index and was found to be significant in obese subjects (Body Mass Index ≥
30.0 kg/m2) and more so in the obese subjects with abdominal
adiposity (p = 0.002) as compared to normal-weight subjects. Karl Pearson
coefficient of correlation revealed a significant positive correlation of
glucose-6-phosphate dehydrogenase with Body Mass Index (r = 0.499; p < 0.001)
and malondialdehyde (a biomarker of oxidative stress) (r = 0.736; p < 0.001)
but inverse correlation with adiponectin (r = -0.524; p < 0.001). Thus, we
conclude that increased expression of glucose-6-phosphate dehydrogenase in
obese subjects (more if it is associated with abdominal adiposity) might mediate
the onset of obesity associated metabolic disorders by increasing oxidative
Cite this paper
Sankhla, M. , Mathur, K. and Rathor, J. (2012) Is there any role of glucose-6-phosphate dehydrogenase in obesity induced metabolic disorder. Health, 4, 1530-1536. doi: 10.4236/health.2012.412A219.
 Zimmet, P., Alberti, K.G. and Shaw, J. (2001) Global and so-cietal implications of the diabetes epidemic. Nature, 414, 782-787. doi:10.1038/414782a
 Nandeesha, Sathiyapriya, V., Bobby, Z., Selvaraj, N., Pavithran, P. and Agarawal, A. (2008) Atherogenic lipid risk factors in men classified as overweight and obese according to the preliminary WHO guidelines for Asians. Indian Journal of Physiology and Pharmacology, 52, 205-208.
 Grundy, S.M., Brewer Jr., H.B., Cleeman, J.I., Smith Jr., S.C. and Lenfant, C. (2004) Definition of metabolic syn- drome: Report of the National Heart, Lung and Blood In- stitute/American Heart Association, Conference on scien- tific issue related to definition. Circulation, 109, 433-438.
 Spiegelman, B.M. and Filer, J.S. (2001) Obesity and the regulation of energy balance. Cell, 104, 531-543.
 Montague, C.T. and O’ Rahilly, S. (2000) The perils of portliness: Causes and con-sequences of visceral adiposity. Diabetes, 49, 883-888. doi:10.2337/diabetes.49.6.883
 Saltiel, A.R. and Kahn, C.R. (2001) Insulin signaling and the regulation of glucose and lipid metabolism. Nature, 414, 799-806. doi:10.1038/414799a
 Dandona, P., Aliada, A. and Ban-dyopadhay, A. (2004) Inflammation-the link between insulin resistance, obesity and diabetes. Trends in Immunology, 25, 4-7.
 Patel, D.A., Srinivasan, S.R., Xu, J.H., Chen, W. and Berenson, G.S. (2006) Adiponectin and its correlates of cardiovascular risk in young adults: The Boga-lusa Heart Study. Metabolism, 55, 1551-1557.
 Perticone, F., Ceravolo, R., Candigliota, M., Ventura, G., Iacopino, S., Sinopoli, F. and Mattioli, P.L. (2001) Obe- sity and body fat distribution induce endothelial dysfunc- tion by oxidative stress: Protective effect of Vitamin C. Diabetes, 50, 159-165. doi:10.2337/diabetes.50.1.159
 Finkel, T. and Holbrook, N.J. (2000) Oxidants, oxidative stress and biology of ageing. Nature, 408, 239-247.
 Perreault, M. and Marette, A. (2001) Targeted disruption of inducible nitric oxide synthase protects against obesity-linked insulin resistance in muscle. Nature Medicine, 7, 1138-1143. doi:10.1038/nm1001-1138
 Furukawal, S., Fujita, T., Shi-mabukuro, M., Iwaki, M., Yamada, Y., Nakajima, Y., Nakayama, O., Makishima, M., Matsuda, M. and Shimomura, I. (2004) Increased oxidative stress in obesity and its impact on metabolic syndrome. Journal of Clinical Investigation, 114, 1752-1761.
 Salati, L.M. and Amir-Ahmady, B. (2001) Dietary regulation of expression of Glucose-6-Phosphate De-hydrogenase. Annual Review of Nutrition, 21, 121-140.
 Horton, J.D., Goldstein, J.L. and Brown, M.S. (2002) SREBPS: Activators of the com-plete program of cholesterol and fatty acid synthesis in the liver. Journal of Clinical Investigation, 109, 1125-1131.
 Klet-zien, R.F., Harris, P.K. and Foellmi, L.A. (1994) Glu-cose-6-phosphate dehydrogenase: A “house-keeping” enzyme subject to tissue specific regulation by hormones, nutrients and oxidant stress. FASEB Journal, 8, 174-181.
 Trinder, P. (1969) Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annals of Clinical Biochemistry, 6, 24.
 Buccolo, G. and David, H. (1973) Quantitative determination of serum triglycerides by the use of enzymes. Clinical Chemistry, 19, 476-482.
 Allain, C.C., Poon, L.S., Chan, C.S.G., Richmond, W. and Fu, P.C. (1974) Enzymatic determination of total serum cholesterol. Clinical Chemistry, 20, 470-75.
 Assman, G., Schriewer, H., Schmitz, G. and Hagele, E.O. (1983) Quantification of high density lipoprotein cholesterol by precipitation with phospho-tungstic acid/MgCl2. Clinical Chemistry, 29, 2026-2030.
 Friedwald, W.T., Levy, R.I. and Fredrickson, D.S. (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clinical Chemistry, 18, 499-502.
 Satoh, K. (1978) Serum lipid peroxide in cerebrovascular disorders de-termined by a new colorimetric method. Clinica Chimica Acta, 90, 37-43.
 Makarem, A., et al. (1974) Clinical chemistry. Principles and techniques. In: Henry, R.F., Cannon, D.C., Winkelman, J.W., Eds., 2nd Edition, Harper and Row, Hagerstown, 1128-1135.
 National Institutes of Health, National Heart Lung and Blood Institute (1998) Clinical guidelines on the identification, evaluation and treatment of overweight and obesity in adults. Obesity Research, 6, 51S-210S.
 WHO Report (2008) Waist circumference and waist-hip ratio. Report of a WHO Expert Consultation, WHO, Geneva.
 Silvio, E.I. (2010) Diabetes facts and guidelines, repot from American Diabetes Association. Diabetes Care, 33, S62.
 Executive summary of the third report of the National Cholesterol Educa-tion Program (NCEP) (2001) Expert panel on detection, evalu-ation and treatment of high blood cholesterol in adults (ATP III). JAMA, 285, 2486- 2497.
 Beutler, E. and Vulliamy, T.J. (2002) Hematologically important mutations: Glu-cose-6-phosphate dehydroge- nase. Blood Cells, Molecules and Diseases, 28, 93-103.
 Dessi, S., Batetta, B., Spano, O., Pulisci, D., Mulas, M.F., Muntoni, S., Armeni, M., Sanna, C., Antonucci, R. and Pani, P. (1992) Serum lipoprotein pattern as modified in G6PD-deficient children during hemolytic anemia induced by fava bean ingestion. International Journal of Experimental Pathology, 73, 157-160.
 Carvalho-Filho, M.A., Ueno, M., Hirabara, S.M., Seabra, A.B., Carvalheira, J.B., De Oliveira, M.G., Velloso, L.A., Curi, R. and Saad, M.J. (2005) S-nitrosation of the insulin receptor, insulin receptor substrate 1, and protein kinase b/akt: A novel mechanism of insulin resistance. Diabetes, 54, 959-967. doi:10.2337/diabetes.54.4.959
 Yasukawa, T., Tokunaga, E., Ota, H., Sugita, H., Martyn, J.A. and Kaneki, M. (2005) S-nitrosylation dependent in- activation of AKt/protein kinase B in insulin resistance. Journal of Biological Chemistry, 280, 7511-7518.
 Park, J., Rho, H.K., Kim, K.H., Choe, S., Lee, Y.S. and Kim, J.B. (2005) Overexpression of Glucose-6-phosphate dehydrogenase is associated with lipid dysregulation and insulin resistance. Molecular and Cellular Biology, 25, 5146-5157. doi:10.1128/MCB.25.12.5146-5157.2005
 Park, J., Choe, S.S., Choi, A.H., Kim, K.H., Yoon, M.J., Suganami, T., Ogawa, Y. and Kim, J.B. (2006) Increase in Glucose-6-phosphate dehydrogenase in adipcyte stimulates oxidative stress and in-flammatory signals. Diabetes, 55, 2939-2949. doi:10.2337/db05-1570
 Arkan, M.C., Hevener, A.L., Greten, F.R., Maeda, S., Li, Z.W., Long, J.M., Wynshaw-Boris, A., Poli, G., Olefsky, J. and Karin, M. (2005) IKK-beta links inflammation to obesity-induced insulin resistance. Nature Medicine, 11, 191-198. doi:10.1038/nm1185
 Cai, D., Yuan, M., Frantz, D.F., Melendez, P.A., Hansen, L., Lee, J. and Shoelson, S.E. (2005) Local and systemic insulin resistance resulting from hepatic activation of IKK-beta and NF-Kappa B. Nature Medicine, 11, 183- 190. doi:10.1038/nm1166