Health  Vol.4 No.12 , December 2012
Effects of methanolic extract of Pausinystalia yohimbe bark on blood glucose level in normal fasting rats
Abstract: Background: Pausinystalia yohimbe (L.) is a member of the family Rubiaceae. It is a valuable medicinal tree, distributed in evergreen closed-canopy forests in West Africa. It is traditionally used for treatment of erectile dysfunction and diabetes. Objective: This was an attempt to evaluate the effects of methanolic extract of P. yohimbe bark on blood glucose level in normal fasting rats. Methods: Different doses of methanolic extract of P. yohimbe bark (5, 10, 20, 40 and 80 mg/kg/mL) were orally administered to normal fasting rats to assess their effects on blood glucose levels. Results: The methanolic extract of P. yohimbe bark in different concentrations (5, 10, 20, 40 and 80 mg/kg/mL) when administered to normal fasting rats, only a considerable reduction (26.57 mg/dL) was produced by the dose of 20 mg/kg/mL. Conclusion and Recommendation: Although P. yohimbe has long been reported to regulate blood glucose levels; such effect is unclear and remains requiring further studies.
Cite this paper: Hassan, H. , Mohamed, T. , Ahmed, E. , Mohamed, A. and Sirag, N. (2012) Effects of methanolic extract of Pausinystalia yohimbe bark on blood glucose level in normal fasting rats. Health, 4, 1225-1228. doi: 10.4236/health.2012.412180.

[1]   Ivorra, M.D., Paya, M. and Villa, A. (1989) A review of natural products and plants as potential antidiabetic drugs. Journal of Ethnopharmacology, 27, 243-275. doi:10.1016/0378-8741(89)90001-9

[2]   Marles, R.J. and Farnsworth, N.R. (1995) Antidiabetic plants and their active constituents. Phytomedicine, 2, 119-189. doi:10.1016/S0944-7113(11)80059-0

[3]   American Diabetes Association (2001) Hyperglycemic crises in patients with diabetes mellitus. Diabetic Care, 24, S83-S90.

[4]   Rates, S.M.K. (2001) Plants as source of drugs. Toxicon, 39, 603-613. doi:10.1016/S0041-0101(00)00154-9

[5]   Okigbo, R.N. and Mmeka, E.C. (2006) An appraisal of phytomedicine in Africa. King Mongkut’s Institute of Technology Ladkrabang Science and Technology Journal, 6, 83-94.

[6]   Bailey, C.J. (1988) Metformin revisited: Its action and indications for use. Diabetes Medication, 5, 315-320. doi:10.1111/j.1464-5491.1988.tb00996.x

[7]   Bordia, M. and Verma, S.K. (1980) Effects of garlic feeding on regression of experimental atherosclerosis in rabbits. Artery, 7, 428-437.

[8]   Ali, M. and Thomson, B. (1995) Consumption of a garlic clove a day could be beneficial in prevention thrombosis. Prostaglandins Leukotrienes & Essential Fatty Acids, 53, 211-212. doi:10.1016/0952-3278(95)90118-3

[9]   Dorant, E., Van den, B., Dorant, P.A. and Goldbohm, R.A. (1995) Allium vegetable consumption, garlic supplement intake, and female breast carcinoma incidence. Breast Cancer Research Treatment, 33, 163-170. doi:10.1007/BF00682723

[10]   Eldin, I.M.T., Ahmed, E.M. and AbdElwahab, H.M. (2009) Hypoglycemic activity and regeneration of pancreatic beta-cells produced by Allium cepa in alloxan-induced diabetic rats. Omdurman Journal of Pharmaceutical, 1, 562-568.

[11]   Khan, A., Mahpara, S. and Mohamed, M. (2003) Cinnamon improves glucose and lipids of people with type 2. Diabetes Care, 26, 3215-3218. doi:10.2337/diacare.26.12.3215

[12]   Tam, S.W., Worcel, M. and Wyllie, M. (2001) Yohimbine: A clinical review. Pharmacology and Therapeutics, 9, 215-243. doi:10.1016/S0163-7258(01)00156-5

[13]   Aantaa, Riku (2000). Alpha2-adrenoceptor antagonists. Baillière’s Clinical Anesthesiology 14 (2): 285-292.

[14]   Abdel-Zaher, A.O., Ahmed, I.T. and El-Koussi, A.D. (2001) The potential antidiabetic activity of some alpha-2 adrenoceptor antagonists. Pharmacological Research, 44, 397-409. doi:10.1006/phrs.2001.0870

[15]   O’Byrne, S. and Feely, J. (1990) Effects of drugs on glucose tolerance in non-insulin-dependent diabetics (Part I). Drugs, 40, 6-18. doi:10.2165/00003495-199040010-00002

[16]   Coull, J.T. (1994) Pharmacological manipulation of the alpha2-noradrenergic system. Effects on cognition. Drugs and Aging, 5, 116-126. doi:10.2165/00002512-199405020-00005

[17]   Grossman, E., Rosenthal, T., Peleg, E., Holmes, C. and Goldstein, D.S. (1993) Oral yohimbine increases blood pressure and sympathetic nervous outflow in hypertensive patients. Journal of Cardiovascular Pharmacology, 22, 22-26. doi:10.1097/00005344-199307000-00004

[18]   Venn, R.M., Bradshaw, C.J. and Spencer, R. (1999) Preliminary UK experience of dexmedetomidine, a novel agent postoperative sedation in the intensive care unit. Anesthesia, 54, 1136-1142. doi:10.1046/j.1365-2044.1999.01114.x

[19]   Rang, H.P., Dale, M.M., Ritter, J.M. and Flower, R.J. (2007) Drugs affecting major organ systems. 6th Edition, Churchill Livingstone, London.

[20]   Schafers, R.F., Nurnberger, J. and Herrmann, B. (1999) Adrenoceptors mediating the cardiovascular and metabolic effects of alpha-methylnoradrenaline in humans. Journal of Pharmacology and Experimental Therapeutics, 289, 918-925.

[21]   Zia, T., Hasnain, S.N. and Hassan, S.K. (2001) Evaluation of the oral hypoglycemic effect of Trigonella foenumgraecum L. (methi) in normal mice. Journal of Ethnopharmacology, 75, 191-195. doi:10.1016/S0378-8741(01)00186-6

[22]   Katzung, G.B. (2004) Basic and Clinical Pharmacology. Endocrine Drugs. 9th Edition, McGraw-Hill, Philadelphia.

[23]   Berridge, T.L., Collins, P.D. and Roach, A.G. (1989) Effects of efaroxan, an alpha-2 adrenoceptor antagonist, on insulin release in conscious rats. British Journal of Pharmacology, 96, 288P-293P.

[24]   Ann, T.P. (1989) Determination of Clinical Glucose, Serum Cholesterol and Insulin levels. Clinical Biochemistry, 6, 24-28.

[25]   Angel, I., Bidet, S. and Langer, S.Z. (1988) Pharmacological characterization of the hyperglycemia induced by alpha2-adrenergic agonists. Journal of Pharmacology and Experimental Therapeutics, 246, 1098-1103.

[26]   Aantaa, R. and Scheinin, M. (1993) Alpha2-adrenergic agents in anaesthesiology. Acta Anaesthesiologica Scandinavica, 37, 433-448.

[27]   Maze, M. and Tranquilli, W. (1991) Alpha-2-adrenoceptor agonists: Defining the role in clinical anesthesia. Anesthesiology, 74, 581-605. doi:10.1097/00000542-199103000-00029

[28]   Andrade, S.F., Sakate, M., Laposy, C.B. and Sangiorgio, F. (2006) Yohimbine and atipamezole on the treatment of experimentally induced amitraz intoxication in cats. The International Journal of Applied Research in Veterinary Medicine, 4, 200-208.

[29]   Ribes, G., Dominique, H.B., Gross, R., Blayac, J.P. and Marie-Madeleine, L.M. (1989) Involvement of a central nervous pathway in yohimbine-induced insulin secretion. European Journal of Pharmacology, 162, 207-214. doi:10.1016/0014-2999(89)90283-5

[30]   Nakadate, T., Muraki, T. and Kato, R. (1980) Effect of α- and β-adrenergic blockers on chlorpromazine-induced elevation of plasma glucose and cyclic AMP in fed mice. Japan Journal of Pharmacology, 30, 199-206. doi:10.1254/jjp.30.199

[31]   Clutter, W.E., Rizza, R.A., Gerich, J.E. and Cryer, P.E. (1988) Regulation of glucose metabolism by sympathochromaffin catecholamines. Diabetes/Metabolism Review, 4, 1-15.