ABSTRACT Chitin nanofibers (CNFs) processed from second most abundant biomolecule chitin were loaded with gold metallic nanoparticles (Au NPs) to obtained hybrid organic-inorganic composite which was molded in different forms dispersion, flakes, powder, and transparent thin film. First pre-organized Au NPs were prepared in soluble triblock copolymer poly (methyl vinyl ether) (PMVE) by reduction of gold precursor salt (HAuCl43H2O) by NaBH4 then NPs were mixed with diluted CNFs suspension to obtain CNFs-Au NPs composite. CNFs of width 25 - 40 nm were prepared by combination of chemical and mechanical processing in wet acidic condition from 1 wt% crab shell chitin slurry. When polymer stabilized Au NPs blended with CNF suspension, all Au NPs and 56% polymer were found mass transferred from water phase to entangle with more polar moieties of chitin. Composite’s suspension and compressed dried film were characterized by recording digital images, UV-vis, TEM, SEM, and XRD spectroscopies. When 70 m thin non transparent composite film was impregnated with tricyclodecane dimethanol dimethacrylate (TCDDMA) resin and subsequent polymerized with photoinitiator 2-hydroxy-2-methylpropiophenone, the film became transparent due to filling of nanosized CNFs in the cages of the resin.
Cite this paper
Shervani, Z. , Taisuke, Y. , Ifuku, S. , Saimoto, H. and Morimoto, M. (2012) Preparation of Gold Nanoparticles Loaded Chitin Nanofiber Composite. Advances in Nanoparticles, 1, 71-78. doi: 10.4236/anp.2012.13010.
 S. Ifuku, M. Nogi, K. Abe, M. Yoshioka, M. Morimoto, H. Saimoto and H. Yano, “Preparation of Chitin Nanofibers with a Uniform Width as Chitin from Crab Shells,” Biomacro-Molecules, Vol. 10, No. 6, 2009, pp. 1584- 1588.
 S. Ifuku, M. Nogi, M. Yoshioka, M. Morimoto, H. Yano and H. Saimoto, “Simple Preparation Method of Chitin Nanofibers with a Uniform Width of 10-20 nm from Prawn Shell under Neutral Conditions,” Carbohydrate Polymers, Vol. 84, No. 2, 2011, pp. 762-764.
 N. K. Gopalan and A. Dufresne, “Crab Shell Chitin Whisker Reinforced Natural Rubber Nanocomposites. 1. Processing and Swelling Behavior,” Biomacromolecules, Vol. 4, No. 3, 2003, pp. 657-665.
 K. Azuma, T. Osakia, T. Wakuda, S. Ifuku, H. Saimoto, T. Tsuka, T. Imagawa, Y. Okamoto and S. Minami, “Beneficial and Preventive Effect of Chitin Nanofibrils in a Dextran Sulfate So-Dium-Induced Acute Ulcerative Colitis Model,” Carbohydrate Polymers, Vol. 87, No. 2, 2012, pp.1399-1403.
 Y. Sueyoshi, T. Hashimoto, M. Yoshikawa and S. Ifuku, “Chitin Nanofiber Membranes for Chiral Separation,” Sustainable Agriculture Research, Vol. 1, No. 1, 2012, pp. 42-47. doi:10.5539/sar.v1n1p42
 S. Ifuku, A. Ikuta, T. Hosomi, S. Kanaya, Z. Shervani, M. Morimoto and H. Saimoto, “Preparation of Polysilsesquioxane-Urethaneacrylate Copolymer Film Reinforced with Chitin NanofiBers,” Carbohydrate Polymers, Vol. 89, No. 3, 2012, pp. 865-869.
 S. Ifuku, M. Tsuji, M. Morimoto, H. Saimoto and H. Yano, “Synthesis of Silver Nanoparticles Templated by TEMPO-Mediated Oxidized Bacterial Cellulose Nanofibers,” Biomacro-Molecules, Vol. 10, No. 9, 2009, pp. 2714-2717. doi:10.1021/bm9006979
 S. Ifuku, K. Yamada, M. Morimoto and H. Saimoto, “Nanofibrillation of Dry Chitin Powder by Star Burst System,” Journal of Nanomaterials, Vol. 2012, 2012.
 S. Ifuku and H. Saimoto, “Chitin Nanofibers: Preparations, Modification, and Applications,” Nanoscale, Vol. 4, No. 11, 2012, pp. 3308-3318.
 S. Ifuku, M. Iwasaki, M. Morimoto and H. Saimoto, “Polymerization of Acrylic Acid onto Chitin Nanofibers to Improve Dispersibility in Basic Water,” Carbohydrate Polymers, Vol. 90, No. 1, 2012, pp. 623-627.
 K. Azuma, T. Osaki, T. Wakuda, S. Ifuku, M. Saimoto, T. Tsuka, T. Imagawa, Y. Okamoto and S. Minami, “Beneficial of and Preventive Effect of Chitin Nanofibers in a Dextran Sulfate Sodium Induced Acute Ulcerative Colitis Model,” Carbohydrate Polymers, Vol. 87, No. 2, 2012, pp. 1399-1403.
 S. Ifuku, Z. Shervani, M. Mrimoto and M. Saimoto, “Processing, Properties, and Applications in Biopolymer Nanocom-Posites,” In: A. Dufresne, S. Thomas and L. A. Pothan, Eds., Wiley Series on Polymer Engineering and Technology, John Wiley & Sons, Inc., Hoboken, 2012.
 S. Ifuku, Z. Shervani and H. Saimoto, “Preparation, Modification and Application of Chitin Nanofibers,” In: W. D. Li and X. P. Wang, Eds., Nanofibers: Synthesis, Properties, and Applications, Nova Scientific Publishers, New York, 2012.
 S. Rana, A. Bajaj, R. Mout and V. M. Rotello, “Monolayer Coated Gold Nanoparticles for Delivery Applications,” Advanced Drug Delivery Reviews, Vol. 64, No. 2, 2011, pp. 200-216.
 D. T. Nguyen, D. Kim and K. Kim, “Controlled Synthesis and Biomolecular Probe Application of Gold Nanoparticles,” Micron, Vol. 42, No. 3, 2011, pp. 207-227.
 A. E. Deniz, H. A. Vural, B. Ortac and T. Uyar, “Gold Nanoparticles/Polymer Nanofibrous Composites by Laser Ablation and Electrospinning,” Materials Letters, Vol. 65, No. 19, 2011, pp. 2941-2943.
 S. Kundu, R. S. Gill and R. F. Saraf, “Electrospinning of PAH Nanofibers and Deposition of Au NPs for Nanodevice Fabrication” Journal of Physical Chemistry C, Vol. 115, No. 32, 2011, pp. 15845-15852.
 A. B. Etame, C. A. Smith, W. C. W. Chan and J. T. Rutka, “Design and Potential Application of Pegylated Gold Nanoparticles with Size-Dependent Permeation through Brain Microvas-Culature,” Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 7, No. 6, 2011, pp. 992-1000. doi:10.1016/j.nano.2011.04.004
 S. Ifuku, M. Tsuji, M. Morimoto, H. Saimoto and H. Yano, “Synthesis of Silver Nanoparticles Template by Tempo-Mediated Oxidized Bacterial Cellulose Nanofibers,” Biomacromoles, Vol. 10, No. 8, 2009, pp. 2714- 2717.
 P. Dallas, V. K. Sharma and R. Zboril, “Silver Polymeric Nanocomposites as Advanced Antimicrobial Agents: Classification, Synthetic Paths, Applications, and Perspectives,” Advances in Colloid and Interface Science, Vol. 166, No. 1-2, 2011, pp. 119-135.
 K. Azuma, T. Osaki, T. Wakuda, S. Ifuku, H. Saimoto, T. Tsuka, T. Imagawa, Y. Okamoto and S. Minami, “Beneficial and Preventive Effect of Chitin Nanofibrils in a Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis Model,” Carbohydrate Polymers, Vol. 87, No. 2, 2012, pp. 1399-1403. doi:10.1016/j.carbpol.2011.09.036
 K. H. L. Kwan, X. Liu, M. K. T. To, K. W. K. Yeung, C. Ho and K. K. Y. Wong, “Modulation of Collagen Alignment by Nanoparticles Results in Better Mechanical Properties in Wound Healing,” Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 7, No. 4, 2011, pp. 497-504.
 D. P. Cormode, P. A. Jarzyna, W. J. M. Mulder and Z. A. Fayad, “Modified Natural Nanoparticles as Contrast Agents for Medical Imaging,” Advanced Drug Delivery Reviews, Vol. 62, No. 3, 2010, pp. 329-338.
 W. Yoon, K. Jung, J. Liu, T. Duraisami, R. Revur, F. T. Teixeira, S. Sengupta and P. R. Berger, “Plasmonenhanced Optical Absorption and Photocurrent in Organic Bulk Hetero-Junction Photovoltic Devices Using Self-Assembled Layer of Silver Nanoparticles,” Solar Energy Materials and Solar Cells, Vol. 94, No. 2, 2010, pp. 128- 132.
 Z. Shervani and Y. Yamamoto, “Soluble Polymer Directed Controlled Synthesis of Gold Nanoparticles: Effect of Cloud Point, Reducing Agents and Mixing Methods on the Nanoparticles Preparation,” International Journal of Nanoscience, Vol. 11, No. 1, 2012, pp. 57-66.
 Z. Shervani and Y. Yamamoto, “Size and Morphology Controlled Synthesis of Gold Nanoparticles in Green Solvent,” Materials Letters, Vol. 65, No. 1, 2011, pp. 92- 95.
 Z. Shervani and Y. Yamamoto, “Carbohydrate Directed Synthesis of Silver And Gold Nanoparticles: Effect of the Structure of Carbohydrates and Reducing Agents on the Size and Morphology of the Composites,” Carbohydrate Research, Vol. 346, No. 5, 2011, pp. 651-658.