Schiff base SH11 with tuberculostatic and radical scavenging activities against INH-induced oxidative hepatic damage

Nedyalka  Georgieva; Zvezdelina  Yaneva; Galina  Nikolova; Svetlana  Simova

doi:10.4236/abb.2012.327130

Nedyalka Georgieva^*, Zvezdelina Yaneva, Galina Nikolova, Svetlana Simova

Abstract: By in vitro visible electron paramagnetic resonance (EPR) spectrophotometry method we demonstrated that N-isonicotinoyl-N’-(3-etoxy-2-hydroxybenzaldehyd) hydrazone (SH11) exhibits radical scavenging activity (SSA). Malondialdehyde (MDA) in mice treated with INH was increased (2.578 ± 0.349 mM vs. 2.024 ± 0.164 μM, p < 0.001), while both superoxide dismutase (SOD) (1.583 ± 0.562 USOD/mg Pr vs. 2.273 ± 0.317 USOD/mg Pr, p < 0.05) and catalase (CAT) (30.176 ± 7.300 UCAT/mg Pr, vs. 47.070 ± 16.490 UCAT/mg Pr, p < 0.05) were decreased, compared to the untreated controls. The combination INH + SH11 (30 mg/kg p.o.) showed reduced levels of MDA, compared to the INH-treated (mean 2.291 ± 0.025 μM than 2.578 ± 0.349, p < 0.05). The combination with the lowest reduction of SOD compared to the controls was 151 mg/kg i.p. INH + 30 mg/kg p.o. SH11, but there was a significant difference in SOD activities between the group treated with this combination and the untreated controls (p < 0.05). The most effective combination, with CAT levels, close to the controls was 151 mg/kg i.p. INH + 30 mg/kg p.o. SH11.

Keywords: INH; Schiff Base; Tuberculosis; EPR; Lipid Peroxidation; Antioxidant Enzymes

Cite this paper: Georgieva, N. , Yaneva, Z. , Nikolova, G. and Simova, S. (2012) Schiff base SH11 with tuberculostatic and radical scavenging activities against INH-induced oxidative hepatic damage. Advances in Bioscience and Biotechnology, 3, 1068-1075. doi: 10.4236/abb.2012.327130.

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