JACEN  Vol.1 No.1 , November 2012
In vitro antagonistic potential of Streptomyces sp. AM-S1 against plant and human pathogens
Abstract: In the present investigation, a total number of 132 different actinomycetes strains were isolated from the humus soil samples. Out of 132 isolates, 52 showed inhibitory activity against the fungal pathogen Rhizoctonia solani. Among the antagonists, the isolate designated as AM-S1 exhibited maximum inhibitory activity against the test pathogen R. solani (41 mm). Further, the light microscopic observations of the co-cultures showed severe structural alterations in the mycelia of R. solani near the zone of inhibition. The isolate AM-S1 was identified as Streptomyces sp. by morphological and 16S rDNA sequence analysis. The color of the aerial and substrate mycelia produced by the Streptomyces sp. AM-S1 varied with different media. The isolate Streptomyces sp. AM-S1 also effectively inhibited the growth of various plant and human pathogens. Further works are needed on optimization of this strain’s antagonistic activity, isolation and characterization of the antimicrobial metabolite.

Cite this paper: K. Sowndhararajan and S. Kang, "In vitro antagonistic potential of Streptomyces sp. AM-S1 against plant and human pathogens," Journal of Agricultural Chemistry and Environment, Vol. 1 No. 1, 2012, pp. 41-47. doi: 10.4236/jacen.2012.11007.

[1]   Oskay, M. (2009) Antifungal and antibacterial compounds from Streptomyces strains. African Journal of Biotechnology, 8, 3007-3017.

[2]   McCarthy, A.J. and Williams, S.T. (1990) Methods for studying the ecology of actinomycetes. Methods in Microbiology, 22, 533-563.

[3]   El-Tarabily, K. and Sivasithamparam, K. (2006) Non-streptomycete actinomycetes as biocontrol agents of soilborne fungal plant pathogens and as plant growth promoters. Soil Biology and Biochemistry, 38, 1505-1520. doi:10.1016/j.soilbio.2005.12.017

[4]   Ilic, S.B., Konstantinovic, S.S., Todorovic, Z.B., Lazic, M.L., Veljkovic, V.B., Jokovic, N. and Radovanovic, B.C. (2007) Characterization and antimicrobial activity of the bioactive metabolites in streptomycete isolates. Microbiology, 76, 421-428. doi:10.1134/S0026261707040066

[5]   Solanki, R., Khanna, M. and Lal, R. (2008) Bioactive compounds from marine actinomycetes. Indian Journal of Microbiology, 48, 410-431. doi:10.1007/s12088-008-0052-z

[6]   Garcia, I., Job, D. and Matringe, M. (2000) Inhibition of p-hydroxyphenylpyruvate dioxygenase by the diketonitrile of isoxaflutole: A case of half-site reactivity. Biochemistry, 3, 7501-7507. doi:10.1021/bi000135h

[7]   Pospiech, A. and Neumann, B. (1995) A versatile quick-prep of genomic DNA from grampositive bacteria. Trends in Genetics, 11, 217-218. doi:10.1016/S0168-9525(00)89052-6

[8]   Kim, H.J., Lee, S.C. and Hwang, B.K. (2006) Streptomyces cheonanensis sp. Nov., a novel streptomycete with antifungal activity. International Journal of Systematic and Evolutionary Microbiology, 56, 471-475. doi:10.1099/ijs.0.63816-0

[9]   Jeanmougin, F., Thompsona, J.D., Gouy, M., Higgins, D.G. and Gibson, T.J. (1998) Multiple sequence alignment with Clustal X. Trends in Biochemical Sciences, 23, 403-405. doi:10.1016/S0968-0004(98)01285-7

[10]   Kumar, S., Nei, M., Dudley, J. and Tamura, K. (2008) MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequences. Briefings in Bioinformatics, 9, 299-306. doi:10.1093/bib/bbn017

[11]   Hayakawa, M., Otoguro, M., Takeuchi, T., Yamazaki, T. and Iimura, Y. (2000) Application of a method incorporating differential centrifugation for selective isolation of motile actinomycetes in soil and plant litter. Antonie van Leeuwenhoek, 78, 171-185. doi:10.1023/A:1026579426265

[12]   Goodfellow, M. and Williams, S.T. (1983) Ecology of actinomycetes. Annual Review of Microbiology, 37, 189-216. doi:10.1146/annurev.mi.37.100183.001201

[13]   Garbeva, P., Postma, J., Veen, J.A. and Elsas, J.D. (2006) Effect of above-ground plant species on soil microbial community structure and its impact on suppression of Rhizoctonia solani AG3. Environmental Microbiology, 8, 233-246. doi:10.1111/j.1462-2920.2005.00888.x

[14]   Huang, X., Zhang, N., Yong, X., Yang, X. and Shen, Q. (2012) Biocontrol of Rhizoctonia solani damping-off disease in cucumber with Bacillus pumilus SQR-N43. Microbiological Research, 167, 135-143. doi:10.1016/j.micres.2011.06.002

[15]   Getha, K. and Vikineswary, S. (2002) Antagonistic effects of Streptomyces violaceusniger strain G10 on Fusarium oxysporum f.sp. cubense race 4: Indirect evidence for the role of antibiosis in the antagonistic process. Journal of Industrial Microbiology and Biotechnology, 28, 303-310. doi:10.1038/sj.jim.7000247

[16]   Williams, S.T., Goodfellow, M., Alderson, G., Wellington, E.M.H., Sneath, P.H.A. and Sackin, M.J. (1983) Numerical classification of Streptomyces and related genera. Journal of General Microbiology, 129, 1743-1813.

[17]   Jain, P.K. and Jain, P.C. (2007) Isolation, characterization and antifungal activity of Streptomyces sampsonii GS 1322. Indian Journal of Experimental Biology, 45, 203- 206.

[18]   Hamdali, H., Hafidi, M., Virolle, M.J. and Ouhdouch, Y. (2008) Rock phosphatesolubilizing Actinomycetes: Screening for plant growth-promoting activities. World Journal of Microbiology and Biotechnology, 24, 2565-2575. doi:10.1007/s11274-008-9817-0