ABSTRACT Malate dehydrogenase, α-esterase and β-esterase isoenzymes have been analyzed by native polyacryla-mide gel electrophoresis in different tissues (liver, kidney, muscle and hump) of the Arabian Camel, Camelus dromedaries in order to study the tissue specificity of these isoenzymes. Malate dehy-drogenase recorded three fractions while both este-rases recorded two fractions in different studied tissues. Malate dehydrogenase was considered as a key isoenzyme in discriminating among the different studied tissues. The percentage amount of both Mdh-1 and Mdh-2 showed significant difference among the different tissues while Mdh-3 recorded significant variation between muscle and hump tissues. The significant variation in the amount of Mdh isoenzyme (on the level of either total or fractions) among the different studied tissues may reflect the role of this enzyme for energy production in this desert animal. With respect to esterases, the percentage amount for both fractions and the total enzyme did not show significant difference among different tissues except β-Est-2 which was significantly higher in hump than in muscle. This enzyme showed higher thickness and intensity in both liver and kidney than in the muscle and hump indicating its greater activity in both liver and kidney.
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AL-Harbi, M. and Amer, S. (2012) Tissue-specific isoenzyme variations in Arabian camel, Camelus dromedaries. Advances in Bioscience and Biotechnology, 3, 863-868. doi: 10.4236/abb.2012.37107.
 Souilem, O. and Barhoumi, K. (2009) Physiological Particularities of Dromedary (Camelus dromedarius) and experimental implications. Scandinavian Journal of Laboratory Animal Science, 36, 19-29.
 Faye, B. and Bonnet, P. (2012) Camel sciences and economy in the world: Current situation and perspectives. In: Johnson, E.H., et al., Eds., Third Proceeding ISOCARD Conference, Muscat, Sultanate of Oman, 2-15. Sultanate of Oman.
 Azwai, S.M., Saltani, H., Thomas, P.C., Shareha, A.M., El Gammudi, F. and Mohamed, S.O. (1990) Note on cholesterol, glucose, urea and total protein concentration in serum of normal camels. Camel Newsletter, 7, 94.
 Al-Qarawi, A.A. and Ali, B.H. (2003) Variations in the normal activity of esterases in plasma and liver of Camels (Camelus dromedarius), Cattle (Bos indicus), Sheep (Ovis aries) and Goats (Capra hircus). Journal of Veterinary Medicine, 50, 201-203.
 Badryyah, A., Al-Suwaigh, R. and Al-Suhaimi A. (2005) Comparative study on some biochemical constituents of plasma in male camels and goats. Journal of Camel Practice and Research, 12, 141-143.
 Elkhawad, A.O. (1992) Selective brain cooling in desert animals: The Camel (Camelus dromedarius). Comparative Biochemistry and Physiology, 101, 195-202.
 Dereje, M. and Udén, P. (2005) The browsing dromedary camel. Behaviour, plant preference and quality of forage selected. Animal Feed Science and Technology, 121, 297-308. doi:10.1016/j.anifeedsci.2005.01.017
 Qari, S.A. and AL-Harbi, M.S. (2011) Effect of seasonal temperatures on physiology and behavior of Arabian Camels (Camelus dromedarieus) in the Mid-Western Region of Saudi Arabia. Glob al Veterinaria, 6, 537-541.
 Ahmed, O.M., Ben Salem, F., Bedhiaf, S., Rekik, B. and Djemali, M. (2010) Genetic diversity in Tunisian dromedary (Camelus dromedarius) populations using microsatellite markers. Livestock Science, 132, 182-185.
 Abdallah, H.R. and Faye, B. (2012) Phenotypic classification of Saudi Arabian camel (Camelus dromedarius) by their body measurements. Emirates Journal Food Agriculture, 24, 272-280.
 Almathen, F., Mwaracharo, J. and Hanotte, O. (2012) Genetic diversity and relationships of indigenous Saudi Arabia camel Camelus dromedarius populations. In: Johnson, E.H. et al., Eds., Third Proceeding ISOCARD Conference, Muscat, Sultanate of Oman, 40-41.
 Abbas, B., Al-Qarawi, A.A. and Al-Hawas, A. (2000) Survey on camel husbandry in Qassim region, Saudi Arabia: Herding strategies, productivity and mortality. Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux, 53, 293-298.
 Shorepy, S.A. (2011) Identification of environmental factors affecting the racing performance of race camels in the United Arab Emirates. Emirates Journal of Food Agriculture, 23, 424-430.
 Markert, C.L. and Moller, F. (1959) Multiple forms of enzymes tissue, ontogenetic and species specific pattern. Proceeding of the Natural Academy of Sciences, 45, 753- 763. doi:10.1073/pnas.45.5.753
 Minarik, P., Tomaskova, N., Kollarova, M. and Antalik, M. (2002) Malate dehydrogenases—structure and function. General Physiology and Biophysics, 21, 257-265.
 Musrati, R.A., Kollarova, M., Mernik, N. and Mikulasova, D. (1998) Malate dehydrogenase: Distribution, function and properties. General Physiology and Biophysics, 17, 193-210.
 Callaghan, A., Boiroux, V., Raymofld, M. and Pasteur, N. (1994) Prevention of changes in electrophoretic mobility of overproduced esterase from organophosphate-resistant mosquitoes of the Culex pipiens complex. Medical Veterinary and Entomology, 8, 391-394.
 Shahjahan, R.M., Karim, A., Begum, R.A., Alam, M.S. and Begum, A. (2008) Tissue specific esterase isozyme banding pattern in Nile Tilapia (Oreochromis niloticus). University Journal of Zoology (Rajshahi University), 27, 1-5.
 Stegemann, H., Afify, A.M.R. and Hussein, K.R.F. (1985) Cultivar identification of dates (Phoenix dactylifera) by protein patterns. Second International Symposium of Biochemical Approaches to Identification of Cultivars, Brounschweig, Germany, 44.
 Jonathan, F.W. and Wendel, N.F. (1990) Visualization and interpretation of plant isozymes. In: Soltis, D.E. and Soltis, P.S., Eds., Isozymes in plant biology, Champan and Hall, London, 5-45.
 Scandaliojs, G. (1964) Tissue-specific isozyme variations in maize. Journal of Heredity, 55, 281-285.
 Allendorff, W. and Utter, F.M. (1978) Population genetics. In: Hoaran, W.S. and Randalal, D.J., Eds., Fish physiology 8, Academic Press, New York, 407-454.
 Lo, A.S.-Y., Liew, C.-T., Ngai, S.-M., Tsui, S.K.-W., Fung, K.-P., Lee, C.-Y. and Waye, M.M.-Y. (2005) Developmental regulation and cellular distribution of human cytosolic malate dehydrogenase (MDH1). Journal of Cell Biochemistry, 94, 763-773. doi:10.1002/jcb.20343
 Danis, P. and Farkas, R. (2009) Hormone-dependant and hormone-independant control of metabolic and developmental functionals of malate dehydrogenase. Endocrine Regulations, 43, 39-52.
 Ahasan, M., Rahman, A., Hossain, S., Mazid, S.Z.S. and Hossain, A. (2011) Beta substrate in esterase enzyme of mosquito fish, Gambusia affinis. Bangladesh Pharmaceutical Journal, 14, 53-59.
 Cowan, K.J. and Storey, K.B. (2001) Tyrosine kinases and phosphatases in the estivating spadefoot toad. Cellular Physiology and Biochemistry, 11, 161-172.