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 AS  Vol.3 No.7 , November 2012
The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels
Abstract: The study was conducted to investigate the optimal hormone treatment for rooting in bitter melon and the effect of defoliation on rooting and polyamine levels. Commercial preparation (diluted 1:10 and 1:20) gave extensive rooting within five days after treatment. The presence of leaf with the stem cutting was necessary for optimal rooting as defoliation drastically reduced rooting even when the rooting time was extended from 5 to 10 days. The presence of leaf also promoted the levels of putrescine three days after the hormone treatment just at the time of root emergence. Cuttings that did not have leaf did not show an increase in putrescine levels and did not produce roots. Spermidine levels also increased in the cuttings that rooted but there was no change in the levels of spermine in the cuttings that rooted. It appears that putrescine and spermidine play a role in regulating rooting in bitter melon cuttings. The vegetative propagation conditions described show that it is a practically viable technique for small growers to produce large number of plantlets at the farm for selection and cultivation.
Cite this paper: Malik, N. , Perez, J. and Kunta, M. (2012) The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels. Agricultural Sciences, 3, 936-940. doi: 10.4236/as.2012.37114.
References

[1]   M Dey, S.S., Singh, A.K., Chandel, D. and Behera, T.K. (2006) Genetic diversity of bitter gourd (Momoridica charantia L.) genotypes revealed by RAPD markers and agronomic traits. Scientia Horticulturae, 109, 21-28. doi:10.1016/j.scienta.2006.03.006

[2]   Al Mansur, M.A.Z., Haque, M.S., Nasiruddin, K.M. and Hossain, M.S. (2009) In vitro propagationof bitter gourd (Momoridica charantia L.) from nodal and root segments. Plant Tissue Culture and Biotechnology, 19, 45-52.

[3]   Jadhav, D.B., Visavale, G.L., Sutar, P.P., Annapure, U.S. and Thorat, (2010) Solar cabinet drying of bitter gourd: Optimizing of pretreatment and quality evaluation. International Journal of Food Engineering, 6, 1-18. doi:10.2202/1556-3758.1503

[4]   Behera, T.K. (2004) Heterosis in bitter gourd. Journal of New Seeds, 6, 217-222. doi:10.1300/J153v06n02_11

[5]   Jayasooriya, A.P., Sakono, M., Yukizaki, C., Kawano, M., Yamamoto, K. and Fukuda, N. (2000) Effect of Momoridica charantia powder on serum glucose levels and various lipid parameters in rat fed with cholesterol-free cholesterol-enriched diets. Journal of Ethnopharmacology, 72, 331-336. doi:10.1016/S0378-8741(00)00259-2

[6]   Han, C., Hui, Q. and Wang, Y. (2008) Hypoglycaemic activity of saponin fraction extracted from Momoridica charantia in PEG/salt aqueous two-phase systems. Natural Product Research, 22, 1112-1119. doi:10.1080/14786410802079675

[7]   Tan, M-J., Ye, J-M., Turner, N., Hohnen-Behrens, C., Ke, C-C., Tang, C-P., Chen, T., Weiss, H-C., Gessing, E-R., Rowland, A., James, D.E. and Ye, Y. (2008) Antidiabetic activities of Triterpenoids isolated from bitter melon associated with activation of the AMPK pathway. Chemistry and Biology, 15, 263-273. doi:10.1016/j.chembiol.2008.01.013

[8]   Shih, C.-C., Lin, C.-H., Lin, W.-L. and Wu, J.-B. (2009) Momoridica charantia extract on insuline resistance and the skeletal muscle GLUT4 protein in fructosefed rats. Journal of Ethnopharmacology, 123, 82-90. doi:10.1016/j.jep.2009.02.039

[9]   Kumar, R., Balaji, S., Uma, T.S. and Sehgal, P.K. (2009) Fruit extracts of Momoridica charantia potentiate glucose uptake and up-regulate Glut-4, PPARγ and P13K. Journal of Ethnopharmacology, 126, 533-537. doi:10.1016/j.jep.2009.08.048

[10]   Guevara, A.P., Lim-Slianco, C., Dayrit, F. and Finch, P. (1990) Antimutagens from Momoridica charantia. Mutation Research, 230, 121-126. doi:10.1016/0027-5107(90)90050-E

[11]   Lee-Huang, S., Huang, P.L, Huang, P.L. Bourinbaiar, A.S., Chen, H.C. and Kung, H.F. (1995) Inhibition of the integrase of human immunodeficiency virus (HIV) type1 by anti HIV plant proteins MAP30, and GAP31. Proceedings of National Academy of Science USA, 92, 8818-8822.

[12]   Reaven, G.M. and Laws, A. (1994) Insulin resistance, compensatory hyperinsulinemia, and coronary heart disease. Diabetologia, 37, 948-952. doi:10.1007/BF00400953

[13]   Manabe, M., Takenaka, R., Nakasa, T. and Okinaka, O. (2003) Induction of anti-inflammatory response by dietary Momoridica charantia. L. (Bitter gourd). Bioscience, Biotechnology, and Biochemistry, 67, 2512-2517. doi:10.1271/bbb.67.2512

[14]   Cefalu, W.T., Ye, J. and Wang, Z.Q. (2008) Efficacy of dietary supplementation with botanicals on carbohydrate metabolism in humans. Endocrine, Metabolic and Immune Disorders—Drug Target, 8, 78-81.

[15]   Nerurkar, P.V., Lee, Y.K., Linden, E. H., Lim, S., Pearson, L., Frank, J. and Nerurkar, V. R. (2006) Lipid lowering effect of Momoridica charantia (Bitter melon) in HIV-1-protease inhibitor-treated human hepatoma cells, HepG2. British Journal of Pharmacology, 148, 115-1164. doi:10.1038/sj.bjp.0706821

[16]   Singh, A., Singh, S.P. and Bamezai, R. (1998) Momoridica charantia (Bitter gourd) peel, pulp, seed and whole fruit extract inhibits mouse skin papillo-magnesis. Toxicology Letters, 94, 37-46. doi:10.1016/S0378-4274(97)00099-4

[17]   Munsur, M.A.Z., Haque, M.S., Nasiruddin, K.M. and Hossain, M.S. (2009) In vitro propagation of bitter gourd (Momoridica charantia L.) from nodal and root segments. Plant Tissue Culture and Bio-technology, 19, 45-52.

[18]   Malek, M.A., Miah, B.M.A. and Al-Amin, M. (2007) In vitro regeneration in pointed gourd. Bangladesh Journal of Agricultural Research, 32, 461-471.

[19]   Tang, Y., Liu, J., Li, X.-M., Liu, B. and Li, H.-X. (2011) The influence of endogenous hormones on the formation of buds from stems of bitter melon (Momoridica charantia L.). African Journal of Biotechnology, 10, 5856-5860.

[20]   Liao, C.T. and Lin, C.H. (1996) Photosynthetic responses of grafted bitter melon seedlings to flood stress. Environmental and Experimental Botany, 36, 167-172. doi:10.1016/0098-8472(96)01009-X

[21]   Wang, S., Tang, L. and Chen, F. (2001) In vitro flowering of bitter melon. Plant Cell Reproduction, 20, 393-397. doi:10.1007/s002990100351

[22]   Rugini, E., Jacoboni, A. and Luppino, M. (1993) Role of basal shoot darkening and exogenous putrescine treatments on invitro rooting and on endogenous polyamine changes in difficult-to-root woody species. Scientia Horticulturae, 53, 63-72. doi:10.1016/0304-4238(93)90138-G

[23]   Hausman, J.-F., Kevers, C. and Gaspar, T. (1994) Involvement of putrescine in the inductive rooting phase of popular shoots raised in vitro. Physiologia Plantarum, 92, 201-206. doi:10.1111/j.1399-3054.1994.tb05327.x

[24]   Malik, N.S.A. and Bradford, J.M. 2007. Different flower-inducing conditions elicit different responses for free polyamine levels in olive Olea europaea leaves. Journal of Japanese Society of Horticulture Science, 76, 205-209. doi:10.2503/jjshs.76.205

[25]   Thimann, K.V. (1977) Hormone action in the whole life of plants. The University of Massachusetts Press, Amherst.

[26]   Rey, M., Diaz-Sala, C. and Rodriguez, R. (1994) Exogenous polyamines improve rooting of hazel microshoots. Plant Cell, Tissue and Organ Culture, 36, 303-308. doi:10.1007/BF00046087

[27]   Jarvis, B.C., Shannon, P.R.M. and Yasmin, S. (1983) Involvement of polyamines with adventitious root development in stem cuttings of mung bean. Plant and Cell Physiology, 24, 677-683.

[28]   Nag, S., Saha, K. and Choudhuri, M.A. (2001) Role of auxin and polyamines in adventitious root formation in relation to changes in compounds involved in rooting. Journal of Plant Growth Regulation, 20, 182-194. doi:10.1007/s003440010016

 
 
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