Recent worldwide foodborne outbreaks emphasize the need for the development of rapid and accurate method for pathogen detection. To address such issues, a new colony based label-free detectionmethodworking on the principles ofelasticlight scattering was introduced. In order to build libraries of scattering images for bacterial pathogens, it is pertinent to determine the effect of preparation and storage of the agar media on the scatter patterns.Scatterpatterns of three Escherichia coli serovars (O26, O111 and O157) were studied and used in a model system, after growth on Sorbitol-MacConkey agarplatesthat were prepared and stored at different conditions in the laboratory.Quantitative image processing software was used to analyzevariationin scatter patterns of the same serovar on media prepared under various standard laboratory conditions and to generate a cross-validation matrix for comparison. Based on the results, it was determined that attention should be given during preparation of media so that theagarplates arenot air-dried more than 10-20 min after solidification at room temperature. Theplatescould be stored in sealed bags in cold room (4oC - 10oC) for up to a month before use.Thefindings of this study should provide guidelines in preparation, storage, and handling of media for generation of reproducible scatterpatternsof bacterial colonies with the light scattering sensor for pathogen detection.
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Mialon, M. , Tang, Y. , Singh, A. , Bae, E. and Bhunia, A. (2012) Effects of Preparation and Storage of Agar Media on the Sensitivity of Bacterial Forward Scattering Patterns. Open Journal of Applied Biosensor, 1, 26-35. doi: 10.4236/ojab.2012.13004.
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